Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   V9W62_RS12200 Genome accession   NZ_CP146763
Coordinates   2578080..2579150 (-) Length   356 a.a.
NCBI ID   WP_043020785.1    Uniprot ID   -
Organism   Bacillus velezensis strain AP81     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2573080..2584150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9W62_RS12150 (V9W62_12150) - 2573139..2573723 (-) 585 WP_015240205.1 signal peptidase I -
  V9W62_RS12155 (V9W62_12155) tapA 2573695..2574366 (-) 672 WP_020956077.1 amyloid fiber anchoring/assembly protein TapA -
  V9W62_RS12160 (V9W62_12160) - 2574625..2574954 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  V9W62_RS12165 (V9W62_12165) - 2574995..2575174 (-) 180 WP_003153093.1 YqzE family protein -
  V9W62_RS12170 (V9W62_12170) comGG 2575231..2575608 (-) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  V9W62_RS12175 (V9W62_12175) comGF 2575609..2576109 (-) 501 WP_257474763.1 competence type IV pilus minor pilin ComGF -
  V9W62_RS12180 (V9W62_12180) comGE 2576018..2576332 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  V9W62_RS12185 (V9W62_12185) comGD 2576316..2576753 (-) 438 WP_043020787.1 competence type IV pilus minor pilin ComGD Machinery gene
  V9W62_RS12190 (V9W62_12190) comGC 2576743..2577051 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  V9W62_RS12195 (V9W62_12195) comGB 2577056..2578093 (-) 1038 WP_043020786.1 competence type IV pilus assembly protein ComGB Machinery gene
  V9W62_RS12200 (V9W62_12200) comGA 2578080..2579150 (-) 1071 WP_043020785.1 competence type IV pilus ATPase ComGA Machinery gene
  V9W62_RS12205 (V9W62_12205) - 2579347..2580297 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  V9W62_RS12210 (V9W62_12210) - 2580443..2581744 (+) 1302 WP_043020784.1 hemolysin family protein -
  V9W62_RS12215 (V9W62_12215) - 2581782..2582612 (-) 831 WP_012117987.1 STAS domain-containing protein -
  V9W62_RS12225 (V9W62_12225) - 2582819..2583199 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  V9W62_RS12230 (V9W62_12230) - 2583437..2583682 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=944515 V9W62_RS12200 WP_043020785.1 2578080..2579150(-) (comGA) [Bacillus velezensis strain AP81]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTQRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=944515 V9W62_RS12200 WP_043020785.1 2578080..2579150(-) (comGA) [Bacillus velezensis strain AP81]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTAAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGGCACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACAACGTGCAGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATTGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379