Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   V9W60_RS11475 Genome accession   NZ_CP146759
Coordinates   2248450..2249520 (-) Length   356 a.a.
NCBI ID   WP_338703651.1    Uniprot ID   -
Organism   Bacillus altitudinis strain JJ61     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2243450..2254520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9W60_RS11425 (V9W60_11425) - 2243539..2244111 (-) 573 WP_017357979.1 signal peptidase I -
  V9W60_RS11430 (V9W60_11430) tapA 2244161..2244688 (-) 528 WP_268432013.1 amyloid fiber anchoring/assembly protein TapA -
  V9W60_RS11435 (V9W60_11435) - 2244959..2245273 (+) 315 WP_234898394.1 DUF3889 domain-containing protein -
  V9W60_RS11440 (V9W60_11440) - 2245308..2245502 (-) 195 WP_008342191.1 YqzE family protein -
  V9W60_RS11445 (V9W60_11445) comGG 2245563..2245946 (-) 384 WP_024718845.1 competence type IV pilus minor pilin ComGG -
  V9W60_RS11450 (V9W60_11450) comGF 2245943..2246440 (-) 498 WP_034647459.1 competence type IV pilus minor pilin ComGF -
  V9W60_RS11455 (V9W60_11455) comGE 2246382..2246696 (-) 315 WP_039166034.1 competence type IV pilus minor pilin ComGE -
  V9W60_RS11460 (V9W60_11460) comGD 2246680..2247126 (-) 447 WP_096881621.1 competence type IV pilus minor pilin ComGD -
  V9W60_RS11465 (V9W60_11465) comGC 2247119..2247412 (-) 294 WP_024718841.1 competence type IV pilus major pilin ComGC Machinery gene
  V9W60_RS11470 (V9W60_11470) comGB 2247429..2248469 (-) 1041 WP_335364405.1 competence type IV pilus assembly protein ComGB -
  V9W60_RS11475 (V9W60_11475) comGA 2248450..2249520 (-) 1071 WP_338703651.1 competence type IV pilus ATPase ComGA Machinery gene
  V9W60_RS11480 (V9W60_11480) - 2249979..2250851 (-) 873 WP_024718838.1 STAS domain-containing protein -
  V9W60_RS11490 (V9W60_11490) - 2251077..2251457 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  V9W60_RS11495 (V9W60_11495) - 2251670..2251912 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  V9W60_RS11500 (V9W60_11500) - 2251961..2252599 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  V9W60_RS11505 (V9W60_11505) - 2252760..2252933 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  V9W60_RS11510 (V9W60_11510) - 2252958..2253272 (-) 315 WP_256688515.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40294.66 Da        Isoelectric Point: 9.2173

>NTDB_id=944246 V9W60_RS11475 WP_338703651.1 2248450..2249520(-) (comGA) [Bacillus altitudinis strain JJ61]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCGLYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=944246 V9W60_RS11475 WP_338703651.1 2248450..2249520(-) (comGA) [Bacillus altitudinis strain JJ61]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGTTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTCAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTCG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACATTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACAATGAATGAACTCGAACAAACAATGGTTGC
TATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGGGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.888

100

0.649