Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   RdKW20_RS03370 Genome accession   NZ_CP146693
Coordinates   666709..667248 (-) Length   179 a.a.
NCBI ID   WP_005693799.1    Uniprot ID   -
Organism   Haemophilus influenzae strain Rd-KW20     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 661709..672248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RdKW20_RS03340 (RdKW20_000627) fdx 662112..662453 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  RdKW20_RS03345 (RdKW20_000628) iscX 662453..662647 (+) 195 WP_005656371.1 Fe-S cluster assembly protein IscX -
  RdKW20_RS03350 (RdKW20_000629) - 662698..663312 (-) 615 WP_005693794.1 YfgM family protein -
  RdKW20_RS03355 (RdKW20_000630) hisS 663330..664601 (-) 1272 WP_005693795.1 histidine--tRNA ligase -
  RdKW20_RS03360 (RdKW20_000631) ispG 664611..665717 (-) 1107 WP_005693796.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  RdKW20_RS03365 (RdKW20_000632) - 665726..666637 (-) 912 WP_005693797.1 RodZ family helix-turn-helix domain-containing protein -
  RdKW20_RS03370 (RdKW20_000633) pilF/pilF2 666709..667248 (-) 540 WP_005693799.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  RdKW20_RS03375 (RdKW20_000634) - 667349..668500 (-) 1152 WP_050396688.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  RdKW20_RS03380 (RdKW20_000635) - 668747..669625 (+) 879 WP_005693802.1 D-alanyl-D-alanine carboxypeptidase family protein -
  RdKW20_RS03385 (RdKW20_000636) - 670011..670871 (+) 861 WP_224056855.1 metal ABC transporter substrate-binding protein -
  RdKW20_RS03390 (RdKW20_000637) - 670871..671791 (+) 921 WP_005630123.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20598.25 Da        Isoelectric Point: 8.6216

>NTDB_id=943841 RdKW20_RS03370 WP_005693799.1 666709..667248(-) (pilF/pilF2) [Haemophilus influenzae strain Rd-KW20]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQTAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=943841 RdKW20_RS03370 WP_005693799.1 666709..667248(-) (pilF/pilF2) [Haemophilus influenzae strain Rd-KW20]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAACTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

100

100

1
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447