Detailed information NTG0942722

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: V6986_RS05265
• Genome accession: NZ_CP146358
• Coordinates: 1088544..1092248 (+)
• Length: 1234 a.a.
• NCBI ID: WP_338594505.1
• Uniprot ID: -
• Organism: Bacillus safensis strain yw6
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1083544..1097248

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V6986_RS05250 (V6986_05250)	-	1083850..1084260 (+)	411	WP_039177642.1	DoxX family protein	-
V6986_RS05255 (V6986_05255)	-	1084458..1084904 (-)	447	WP_039177646.1	ferritin-like domain-containing protein	-
V6986_RS05260 (V6986_05260)	addB	1085057..1088566 (+)	3510	WP_247544379.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
V6986_RS05265 (V6986_05265)	addA	1088544..1092248 (+)	3705	WP_338594505.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
V6986_RS05270 (V6986_05270)	sbcD	1092275..1093450 (+)	1176	WP_338594506.1	exonuclease subunit SbcD	-
V6986_RS05275 (V6986_05275)	-	1093447..1096842 (+)	3396	WP_338594508.1	SMC family ATPase	-
V6986_RS05280 (V6986_05280)	-	1096862..1097161 (+)	300	WP_144490894.1	HNH endonuclease	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 142182.26 Da
• Isoelectric Point: 5.0359

>NTDB_id=942722 V6986_RS05265 WP_338594505.1 1088544..1092248(+) (addA) [Bacillus safensis strain yw6]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDKYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQVNELIRHQNDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFEKAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHAADGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFTPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESEDTTTEMLLVHLDQQEAETDEEREELETVQLEARVIAKK
IRELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPIVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEGWLLPDFERYQSKTYLDFIGPALIRHQAMSSILEETGDVVLSHPSAFTISFTQASDL
LKEDMSLEKEQQDEVVQALMEGLPVEQYGDADEKVAERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRKAR
ATLYDRPAFMKKKALTAAEQGTAMHTVMQHIALPSDEPYDDSRIQQLLDSLQQRDLLTNEQVQSIDQEGIAAFFSTSIGQ
KLRKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRFTGGLEAAVPALKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNIEISL

Nucleotide

• Length: 3705 bp

>NTDB_id=942722 V6986_RS05265 WP_338594505.1 1088544..1092248(+) (addA) [Bacillus safensis strain yw6]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAATGGGAAGCCATCGTTTCAGAAGGACAAGATATCCT
TGTTGCGGCGGCAGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCAGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCGAAAAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCGCTCATGAATCGTGCCAATATTTC
TACCTTGCACTCCTTTTGTTTGCAGGTTCTGCGCACCTTTTATTATGAAATTGACCTTGATCCAGGCTTCCGGTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATAAATATAAGGCTGGAAAGCCATCG
TTCTTTGAACTGGTTGATCGCTATACTTCTGACCGCCATGATTTAGACTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCCAGATCTCACCCTTCACCTGAGCAATGGATGAGGGCATTTCTTTCTCTTTATGATGTAGATGCTCAAACAAAAGTAG
AACAATTACCGTTTTACCCATATATCAAAGAGGATCTTTCTCTCGTTTTTCGAAGCTGTCTCGAACTACTCGAACGAGCT
CTCGCCCTATCAAAAGAACCGGGCGGCCCGGCACCGAGAGCAGAAAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCAGGCACCAAAATGATTTTGAAAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGCAATCAAGCAAAAAAACAATTAGAAAAACTTAAA
GATGAATACTTTATGCGCAGTCCAGCCCAGCATTTGAAAAGCTTAGCTGAAATGAAGCCGGTGGTCGAAACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGTTTCGAAAAAGCGAAGCAAGAAAAATCAATCGTGGACTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCAGAGCAGGATGCGGAAGGAAATTTGATCGAAACAGAAGCGGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAAGATACAAACCTTGTACAAGAAACGATTTTAAAGCTTGTATCTAAAGGGGA
ACATGCCGCAGACGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGCTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAATAAATATAAACAATTTACACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTGTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCGAAGATACAACGACAGAAATGCTTCTTGTCCATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAGCGAGAAGAATTAGAGACCGTACAGCTTGAAGCGAGAGTCATTGCTAAAAAA
ATAAGAGAATTGGTGGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAACTTGCAGTATCGGGACAT
TGTGATTTTGCTTCGTTCTATGCCATGGGCCCCGCAAATGATGGAGGAATTGAAGAAGCAGGGAATCCCTGTGTATGCGA
ATCTTTCTTCCGGTTATTTTGAAGCAACAGAGGTGTCTGTGATTCTTTCTCTGTTAAAAGTCATTGATAATCCATATCAG
GATATCCCGCTTGCAGCTGTATTAAGATCACCACTTGTCCATTTAGACGAAAATGAAATGGCACTGATTCGCACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTGAAGGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACGGTTTTTCAGGTTGCTGCGTAAATGGCGTGATTTCTCGATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATTTAGATTACGTTGGCGGGATGCCTGGAGGCAAGCAGAGACAGGCGAATCTCCGCGCTCTTTATGA
TCGAGCGAAGCAGTATGAAAAAGCTGCCTTTAGAGGGCTGTTCCGTTTTCTTCGTTTTATCGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAAGATGTTGTTCGCATGATGACAATTCACAGCAGTAAAGGA
TTAGAATTTCCAATCGTCTTTACCGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTATTTACTTGA
TAAAGAGCTAGGCTTTGGAAGCAAGTATATCCATCCTGAGCTCAGAATTAGTTATGCAACATTACCGCTTGTAGCGATGA
AAAAAAAGATGAGGAAAGAGCTGTTATCTGAAGAGCTGAGAGTTCTATATGTGGCGCTCACAAGAGCGAAGGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAAGGTTGGCTGCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTCCTGCTCTCATCCGTCACCAAGCAATGTCAT
CTATTTTAGAAGAAACAGGTGATGTTGTTCTTTCGCATCCATCAGCATTCACAATCTCATTTACTCAAGCTTCCGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGGAGCAGCAAGACGAGGTGGTACAAGCTTTGATGGAGGGGCTGCCTGTTGAACA
ATATGGTGATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATACCCATATTTAGCGGCCTCACAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAGAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTCGAAAAGCTCGT
GCCACGTTGTATGACCGGCCGGCTTTCATGAAGAAAAAAGCACTCACTGCCGCAGAGCAAGGCACCGCAATGCACACAGT
CATGCAGCATATTGCGCTTCCTTCTGACGAGCCCTACGATGATTCACGTATACAGCAGCTGCTCGATTCCTTACAGCAGC
GAGACTTGCTAACAAATGAGCAAGTCCAGTCAATTGATCAAGAAGGAATTGCAGCTTTCTTCTCTACTTCTATTGGTCAA
AAGCTGCGAAAAGCGGATTGGGTGAAACGAGAAGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAGGGGGAGCCTGTCCTTATTCAAGGGATGATTGACTGTTTATTTGAAACGGACGGAAAGCTATATTTACTAG
ATTATAAAACCGATAGAGTGCAAGGCAGATTTACAGGCGGCCTTGAAGCAGCTGTGCCTGCATTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTATATGCAAAAGCAGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
TGGGAATATAGAAATTTCATTATAA

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-462)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1226)

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	65.94	100	0.662