Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   V6986_RS05260 Genome accession   NZ_CP146358
Coordinates   1085057..1088566 (+) Length   1169 a.a.
NCBI ID   WP_247544379.1    Uniprot ID   -
Organism   Bacillus safensis strain yw6     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1080057..1093566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6986_RS05230 (V6986_05230) lepB 1080574..1081086 (+) 513 WP_039177636.1 signal peptidase I -
  V6986_RS05235 (V6986_05235) - 1081125..1082270 (-) 1146 WP_058838811.1 AbrB family transcriptional regulator -
  V6986_RS05240 (V6986_05240) - 1082538..1082960 (+) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  V6986_RS05245 (V6986_05245) - 1082953..1083792 (+) 840 WP_039177640.1 M56 family metallopeptidase -
  V6986_RS05250 (V6986_05250) - 1083850..1084260 (+) 411 WP_039177642.1 DoxX family protein -
  V6986_RS05255 (V6986_05255) - 1084458..1084904 (-) 447 WP_039177646.1 ferritin-like domain-containing protein -
  V6986_RS05260 (V6986_05260) addB 1085057..1088566 (+) 3510 WP_247544379.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  V6986_RS05265 (V6986_05265) addA 1088544..1092248 (+) 3705 WP_338594505.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  V6986_RS05270 (V6986_05270) sbcD 1092275..1093450 (+) 1176 WP_338594506.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134872.94 Da        Isoelectric Point: 5.4378

>NTDB_id=942721 V6986_RS05260 WP_247544379.1 1085057..1088566(+) (addB) [Bacillus safensis strain yw6]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEEKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISLSSMHSEYTEERRAGE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPLSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDKQPNELDLFRMTGKTYFQLYQLAKECGAAISEHILERNNRHLHTPDLAYLERQYEQRPVQPYQEVTPHLTVSKSASK
RAEIEGVAREILHLVREEGLRLRDISVVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IAPPLFKLQKRLKKAQKVKDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYSVSDEEGRSLLPSTLIKYCQELMPNHQHALYVLDPEQLDDEEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLITSTHRDVSKNILSSLFFTNRAKRLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKDDCVNYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHARVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLSSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=942721 V6986_RS05260 WP_247544379.1 1085057..1088566(+) (addB) [Bacillus safensis strain yw6]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTAGATCCGCTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTCTTAATGGAGTACGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGGATGATCAGGGCCAAAGTATTTAGTTTCACGCGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTAATTGAAGAAGAGAAAGAAAAGTT
TAAGGTATTCAAAAAAGCGAGTGATAAGCCAGGGTTTGTCGAGCAAATCGAAAAGACGATGGCTGAGTTCAAAAGATACT
GCATGTTGCCGGAGGATATTGAAAAAATTTCATTATCGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGGCGAA
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACGCTTCTCACGCAGCAAATTCCTCTATCTGAAGAAATAAAAGGAGCACATATTTATATAGATGGCTTTTATCAATTTA
CACCGCAGCAGCTTCTCGTCATTGAACAGCTTCTGCTTCATGCTGAGAAAATAACGGCTGCGTTCACAGTGGATCGTTCC
TACCATGATAAGCAGCCAAATGAACTTGATTTATTTCGTATGACTGGTAAAACGTATTTCCAGCTCTATCAGCTTGCTAA
AGAGTGCGGAGCGGCCATTTCTGAGCACATTTTGGAGAGAAATAACCGGCATCTTCATACGCCAGATCTTGCTTATTTAG
AACGCCAATATGAGCAGAGACCGGTGCAGCCATATCAGGAAGTAACCCCTCATCTCACAGTGTCTAAAAGTGCGAGTAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGAAATTCTTCATTTAGTGAGAGAAGAAGGACTTAGACTGCGAGATATTTCAGT
TGTCGCGCGGCATGTAGATGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCAATGCAGTATCATCCGCTCATTGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAACTGGCGGTAT
GAGGCCGTGTTTCGCTGCGTGAAAACAGAGTTCTTATTTCCGCTCGAACTTGCCAAAAACAAAGCGAGAGAACAGGCAGA
TCAGCTTGAAAACTACTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGCTCCCGTTTCCACTACAGACGAT
TCCAATCTTTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAGATGCTGAATGATGTAAAGGAATGG
ATTGCACCTCCGCTTTTCAAATTGCAAAAAAGGCTGAAAAAAGCGCAAAAGGTAAAAGACATGGTGGAGGCAGTCTATGT
CTTTTTAGAGGAAATACAAGTGCCAGATAAACTTGAAAAAGCTAGGCTGGAAGCAGAAGAAGCAGGTAGATTAGCTGAAG
CCATGCAGCATGGACAAGTATGGGATGCTGTTATTCAATTAATGGATGAATTTGTTGAAATGCTCGGTGAGGAGGAGCTC
TCATTTCCTTTATTCCAGCAAATGATGGATACAGGATTGGCCTCTTTAAAATTCGCTTTAATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCAAGAATGTATCAAGTGAAGTGTATGTTTATCATTGGGGTAAATGATGGCGTTA
TTCCCGCACGCCCTTCTGACGAAAGTGTCTTGTCTGAGGATGACAGGGAATGGTTAAAGCGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAGGCGCTGTCGAGTCCATCTCATCATTTGTATCT
GTCTTATTCAGTGTCAGATGAAGAGGGGCGCTCTTTATTGCCTTCAACTCTTATCAAGTATTGTCAGGAGCTTATGCCAA
ATCATCAGCATGCTTTGTATGTGTTAGACCCGGAACAGCTGGATGACGAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTCTCGTTATCGTATACTGTCTCGCAGCTGCAGCAATGGCTCAACCAATATCCCATCAGTGGTGTATGGTGGAGTGTTTA
TAATTATTTGATAACGTCAACTCATCGGGATGTATCAAAAAACATCTTGTCGAGTTTGTTTTTTACAAATCGAGCAAAGC
GCTTAAAACCAAACGTCACAAAAGAACTTTACGGTGATCACATTCAAGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGGTTAAAACTAAAGGACCGGCAATTTTATAAATTGGAAGCACCCGATATCGG
CCAGCTGTTTCACTCGGCTTTAAAGCATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACTTAACGAAGG
ATGACTGTGTCAACTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATCCTCTTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAACTCCAAAAAATCTTGATTCGGGTCTCTTCTATCTTAAGTGAGCATGCAAGAGTGAG
TGGATTTTCTCCCGTTGGACTTGAGTTAGGGTTTGGCGGCCAAGGACCTCTTCCGCCGTTTACTTTTCAATTGAAAAATG
GCTGCACGATGGAGCTTGTCGGAAGAATTGACCGCGTAGATAAAGCAGAAGGTTCAAAAGGGCTGTTGTTGAGAATCGTC
GACTACAAATCGAGTGAGAAAGGTCTTGATCTAGCAGAGGTATACTATGGGCTGGCCTTGCAAATGCTCACTTACTTAGA
TTTAACCATTACGTATTCGAAGGAATGGCTCGGTGTAGAAGCAACGCCAGCTGGCATTTTATATTTCCATATCCATGATC
CGCTTATTCAAGCACCGATTCCGCTTGCAGAGGATGAAATTGAGCAGGAAATATTCAAGAAATTTAAAATGAAAGGTCTA
TTGCTTGAAGATGTAGAAGCGGTAAAGCTAATGGATCAAACGCTTGAATCAGGAAGATCACAAGTCATTCAAGCCGGTTT
GAAAAAGGACGGATCTTTCCGTTCTGACTCTGCTGTTTTAAGTGAAGATCATTTCCATATACTCACACAGCACGTTCGGC
GCACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGTGAAGTAGCCATTAATCCATATAAATTGAAGGATCAAACACCA
TGCCGCTTTTGTTCATTTAAGTCTATTTGCCAATTTGATGAATCGATAGAGGATAATGAATTTAGAGTGCTATCATCTGA
AAAGGATGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

65.805

99.316

0.654