Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   PSGK_RS28730 Genome accession   NZ_CP146285
Coordinates   6342448..6344040 (-) Length   530 a.a.
NCBI ID   WP_021219359.1    Uniprot ID   A0AAU7Y5U8
Organism   Pseudomonas solani strain GK13     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 6337448..6349040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSGK_RS28700 (PSGK_28550) - 6337448..6338257 (-) 810 WP_374979898.1 PilW family protein -
  PSGK_RS28705 (PSGK_28555) pilV 6338254..6338829 (-) 576 WP_184488603.1 type IV pilus modification protein PilV -
  PSGK_RS28710 (PSGK_28560) - 6338816..6339334 (-) 519 WP_374979899.1 GspH/FimT family pseudopilin -
  PSGK_RS28715 (PSGK_28565) - 6339449..6339958 (-) 510 WP_374979900.1 GspH/FimT family protein -
  PSGK_RS28720 (PSGK_28570) thiO 6340001..6341098 (+) 1098 WP_374979901.1 glycine oxidase ThiO -
  PSGK_RS28725 (PSGK_28575) pilR 6341111..6342445 (-) 1335 WP_021219360.1 sigma-54 dependent transcriptional regulator Regulator
  PSGK_RS28730 (PSGK_28580) pilS 6342448..6344040 (-) 1593 WP_021219359.1 PAS domain-containing sensor histidine kinase Regulator
  PSGK_RS28735 (PSGK_28585) - 6344030..6344266 (-) 237 WP_350447864.1 PP0621 family protein -
  PSGK_RS28740 (PSGK_28590) - 6344449..6345468 (-) 1020 WP_021219357.1 outer membrane protein assembly factor BamD -
  PSGK_RS28745 (PSGK_28595) rluD 6345617..6346588 (+) 972 WP_021219356.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PSGK_RS28750 (PSGK_28600) pgeF 6346588..6347313 (+) 726 WP_374979902.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58629.90 Da        Isoelectric Point: 8.5806

>NTDB_id=942508 PSGK_RS28730 WP_021219359.1 6342448..6344040(-) (pilS) [Pseudomonas solani strain GK13]
MSAEAPALGSQPARRIFRLYNIYRLLIGLALVGLISSELDSELLDLARAQLFRNACWFYLLLNILIALMVRHPKHMLQVF
SLALVDVILLSCLFYAAGGTPSGIGNLVIVAVAIANILLRGRVGLLIAAVAAIGMIYLTFYLSLSRPEAASQYVQAGALG
ALCFAAALFVQGISKRLQLSETLAEKRAADVANLEALNALILQRMRTGILVLDPQHRVILANQGALTLLGREDLAGKVLD
PQSPELVKRLQQWLDNPTLRPQSLQAVAEGPVLQPSFVSLQRGEQRDTLIFLDDISQIAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELSGPDQRLAQIIQDQSRRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFASEFRNASGPG
QTLHIETNSGTIQTRMDPHQLTQVLTNLVQNGLRYSSQKNKVGQVWLKLFRDPESDLPVLEVMDDGAGVPAEQVQHIFEP
FYTTENKGTGLGLYISRELCESNQARLDYRPRETGGSCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=942508 PSGK_RS28730 WP_021219359.1 6342448..6344040(-) (pilS) [Pseudomonas solani strain GK13]
GTGAGCGCTGAAGCCCCCGCGCTGGGCAGCCAGCCGGCACGCCGCATCTTCCGCCTCTACAACATCTACCGCCTGCTCAT
CGGCCTGGCACTGGTGGGCCTGATCTCCAGCGAGCTGGACAGCGAACTGCTCGACCTGGCCCGCGCCCAGCTGTTCCGCA
ATGCGTGCTGGTTCTACCTGCTGCTGAACATCCTCATCGCGCTGATGGTCCGCCATCCGAAACACATGCTGCAGGTCTTC
AGCCTGGCGCTGGTGGACGTGATCCTGCTGTCCTGCCTGTTCTATGCGGCGGGCGGCACGCCGAGCGGTATCGGCAACCT
GGTGATAGTTGCGGTGGCGATCGCCAACATCCTGCTGCGCGGCCGGGTCGGCTTGCTGATCGCCGCCGTCGCCGCCATCG
GCATGATCTACCTGACCTTCTACCTCAGCCTCAGCCGCCCGGAAGCCGCCAGCCAGTACGTGCAGGCCGGCGCCCTGGGT
GCCTTGTGCTTCGCCGCCGCCCTCTTCGTCCAGGGCATCAGCAAGCGCCTGCAACTGAGCGAGACCCTGGCCGAAAAACG
TGCCGCCGACGTCGCCAACCTGGAAGCGCTCAACGCCCTGATCCTGCAGCGCATGCGCACCGGCATCCTGGTGCTCGACC
CCCAGCACCGCGTAATCCTGGCCAACCAGGGTGCGCTGACGCTGCTGGGCCGCGAGGACCTGGCCGGCAAGGTCCTCGAC
CCGCAAAGCCCGGAGCTGGTGAAACGCCTTCAGCAGTGGCTGGACAACCCCACGCTGCGTCCCCAGAGCCTGCAGGCGGT
GGCCGAAGGCCCGGTGCTGCAACCGAGCTTCGTCTCCCTGCAGCGGGGCGAGCAGCGGGACACCCTGATCTTCCTCGACG
ACATCTCGCAGATCGCCCAACAGGCCCAGCAACTCAAGCTCGCCTCCCTGGGTCGCCTGACCGCCGGCATCGCCCACGAG
ATACGCAACCCACTGGGCGCCATCAGCCACGCGGCGCAGTTGCTGCAGGAGTCCGAGGAGCTGTCCGGTCCCGACCAGCG
CCTGGCGCAGATCATCCAGGACCAGTCGCGGCGTATGAACCTGGTGATCGAGAACGTCCTGCAGCTCTCCCGCCGCCGCC
AAGCGGAGCCGCAATTGCTCGACCTAAAGTACTGGCTGCACCGCTTCGCCAGCGAATTCCGCAACGCATCCGGCCCGGGC
CAGACGCTGCACATCGAGACCAACAGCGGCACCATCCAGACCCGCATGGACCCGCACCAACTCACCCAGGTACTAACCAA
CCTGGTACAGAACGGCCTGCGCTACAGTTCACAGAAGAACAAGGTAGGACAGGTCTGGCTGAAGCTGTTCCGCGACCCGG
AAAGCGACCTGCCCGTTCTGGAAGTGATGGATGACGGCGCCGGCGTACCCGCCGAGCAGGTGCAGCACATCTTCGAGCCC
TTCTACACCACCGAAAACAAGGGCACCGGGCTTGGCCTGTACATCTCACGGGAGCTGTGCGAGAGCAACCAGGCCCGTCT
CGACTACAGGCCCCGCGAAACCGGAGGCAGCTGCTTTCGCATAACCTTTGCCCACCCGCGCAAATTGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

PAS domain (PAS_8)

(202-253)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

65.849

100

0.658