Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   V8H05_RS00410 Genome accession   NZ_CP146213
Coordinates   90389..92017 (-) Length   542 a.a.
NCBI ID   WP_338616604.1    Uniprot ID   -
Organism   Staphylococcus aureus strain KWT 2019-23549     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 41005..93384 90389..92017 within 0


Gene organization within MGE regions


Location: 41005..93384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8H05_RS00190 (V8H05_00190) ccrA 42654..44003 (-) 1350 WP_338616594.1 recombinase family protein Machinery gene
  V8H05_RS00195 (V8H05_00195) - 44237..46030 (-) 1794 WP_338616595.1 DUF927 domain-containing protein -
  V8H05_RS00200 (V8H05_00200) - 46030..46326 (-) 297 WP_000273824.1 single-stranded DNA-binding protein -
  V8H05_RS00205 (V8H05_00205) - 46519..47565 (-) 1047 WP_002454338.1 hypothetical protein -
  V8H05_RS00210 (V8H05_00210) - 47837..48908 (-) 1072 Protein_39 IS3 family transposase -
  V8H05_RS00215 (V8H05_00215) - 49075..54726 (-) 5652 WP_338616598.1 E domain-containing protein -
  V8H05_RS00220 (V8H05_00220) - 55262..55567 (+) 306 WP_000677987.1 hypothetical protein -
  V8H05_RS00225 (V8H05_00225) - 55765..56628 (+) 864 WP_000680579.1 hypothetical protein -
  V8H05_RS00230 (V8H05_00230) - 56736..58209 (+) 1474 Protein_43 DUF6038 family protein -
  V8H05_RS00235 (V8H05_00235) - 58436..59536 (+) 1101 WP_000449985.1 DNA polymerase -
  V8H05_RS00240 (V8H05_00240) - 59529..59900 (+) 372 WP_001058157.1 hypothetical protein -
  V8H05_RS00245 (V8H05_00245) - 59897..61186 (+) 1290 Protein_46 DUF5906 domain-containing protein -
  V8H05_RS00250 (V8H05_00250) - 61231..61905 (-) 675 WP_001105999.1 IS6-like element IS257 family transposase -
  V8H05_RS00255 (V8H05_00255) - 62188..63534 (+) 1347 WP_000163428.1 NAD(P)/FAD-dependent oxidoreductase -
  V8H05_RS00260 (V8H05_00260) merR 63834..64241 (+) 408 WP_000425271.1 Hg(II)-responsive transcriptional regulator MerR -
  V8H05_RS00265 (V8H05_00265) - 64258..64611 (+) 354 WP_000746201.1 TlpA disulfide reductase family protein -
  V8H05_RS00270 (V8H05_00270) - 64608..65288 (+) 681 WP_000010352.1 cytochrome c biogenesis protein CcdA -
  V8H05_RS00275 (V8H05_00275) merT 65376..65762 (+) 387 WP_001794630.1 mercuric transport protein MerT -
  V8H05_RS00280 (V8H05_00280) merA 65820..67463 (+) 1644 WP_000193219.1 mercury(II) reductase -
  V8H05_RS00285 (V8H05_00285) merB 67545..68195 (+) 651 WP_000790333.1 organomercurial lyase MerB -
  V8H05_RS00290 (V8H05_00290) - 68390..68905 (-) 516 WP_000377078.1 hypothetical protein -
  V8H05_RS00295 (V8H05_00295) - 69024..69698 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  V8H05_RS00300 (V8H05_00300) - 69782..70123 (+) 342 WP_001797647.1 hydroxymethylglutaryl-CoA synthase -
  V8H05_RS00305 (V8H05_00305) - 70960..71703 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  V8H05_RS00310 (V8H05_00310) - 71800..72228 (+) 429 WP_000872606.1 MaoC family dehydratase -
  V8H05_RS00315 (V8H05_00315) mecA 72274..74283 (-) 2010 WP_338616602.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  V8H05_RS00320 (V8H05_00320) mecR1 74380..76137 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  V8H05_RS00325 (V8H05_00325) mecI 76137..76508 (+) 372 WP_338616603.1 mecA-type methicillin resistance repressor MecI -
  V8H05_RS00330 (V8H05_00330) psm-mec 76593..76661 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  V8H05_RS00335 (V8H05_00335) - 76981..78129 (+) 1149 WP_150854453.1 ROK family transcriptional regulator -
  V8H05_RS00340 (V8H05_00340) cstB 78243..79577 (-) 1335 Protein_65 persulfide dioxygenase-sulfurtransferase CstB -
  V8H05_RS00345 (V8H05_00345) - 79609..80673 (-) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  V8H05_RS00350 (V8H05_00350) cstR 80809..81069 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  V8H05_RS00355 (V8H05_00355) - 81069..81707 (+) 639 Protein_68 sulfite exporter TauE/SafE family protein -
  V8H05_RS00360 (V8H05_00360) - 81981..82598 (-) 618 WP_000562107.1 CadD family cadmium resistance transporter -
  V8H05_RS00365 (V8H05_00365) - 82679..85093 (-) 2415 WP_000378485.1 cadmium-translocating P-type ATPase CadA -
  V8H05_RS00370 (V8H05_00370) - 85086..85451 (-) 366 WP_000159127.1 metalloregulator ArsR/SmtB family transcription factor -
  V8H05_RS00375 (V8H05_00375) - 85637..86014 (-) 378 WP_001041908.1 DUF6262 family protein -
  V8H05_RS00380 (V8H05_00380) - 86021..87913 (-) 1893 WP_338613460.1 site-specific integrase -
  V8H05_RS00385 (V8H05_00385) - 88108..88431 (-) 324 WP_000088091.1 JAB domain-containing protein -
  V8H05_RS00390 (V8H05_00390) - 88424..88630 (-) 207 WP_001077283.1 hypothetical protein -
  V8H05_RS00395 (V8H05_00395) - 88632..89153 (-) 522 WP_000836008.1 DUF1643 domain-containing protein -
  V8H05_RS00400 (V8H05_00400) - 89172..89483 (-) 312 WP_000833212.1 DUF960 family protein -
  V8H05_RS00405 (V8H05_00405) - 89568..89918 (-) 351 WP_000171449.1 SAUGI family uracil-DNA glycosylase inhibitor -
  V8H05_RS00410 (V8H05_00410) ccrB 90389..92017 (-) 1629 WP_338616604.1 recombinase family protein Machinery gene
  V8H05_RS00415 (V8H05_00415) ccrA 92038..93384 (-) 1347 WP_000816499.1 recombinase family protein Machinery gene

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62274.15 Da        Isoelectric Point: 9.7334

>NTDB_id=942030 V8H05_RS00410 WP_338616604.1 90389..92017(-) (ccrB) [Staphylococcus aureus strain KWT 2019-23549]
MQQLKQKRVGIYVRVSTEIQSTEGYSIDGQINQIREYCDFNNFVVVDVYADRGISGKSMNRPEIQRLLKDANEGQIDSVM
VYKTNRLARNTSDLLKIVEDLHRQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSIGSVTYILSNPFYVGKIQFAK
YKDWNEKRRKGLNDKPIIAEGKHSPIIIQDLWDKVQLRKKQVSQKPQVHGKGTNLLTGIVHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEKYVMDQILEIVKSDKVINQVLERVNQENKVDIGALNHDIAYKQQQYD
EVSGKLHNLVKTIEDNPDLTSALKATIHQYETQLNDITNQMNQLKQQQNQEKLSYDTKQIAALLQRIFQNIESMDKAQLK
ALYLTVIDRIDIRKDGNHKKQFYVTLKLNNEIIKQLFNNTPLDEVLLSTSSLFLPQTLFLQI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=942030 V8H05_RS00410 WP_338616604.1 90389..92017(-) (ccrB) [Staphylococcus aureus strain KWT 2019-23549]
ATGCAACAACTCAAACAAAAACGTGTCGGTATCTATGTTCGTGTATCAACGGAAATCCAAAGTACTGAAGGCTATAGTAT
CGATGGACAAATCAATCAAATTCGAGAATATTGTGATTTCAATAACTTTGTTGTTGTAGATGTATACGCGGATAGAGGTA
TCTCTGGAAAATCTATGAACCGACCAGAAATACAACGTTTGTTAAAAGATGCGAACGAAGGTCAGATTGATTCTGTTATG
GTCTACAAAACAAACCGACTAGCACGTAACACTTCTGACTTACTCAAAATTGTTGAAGACCTTCATCGTCAAAATGTCGA
ATTCTTCAGCTTATCTGAGCGTATGGAAGTCAATACAAGCAGTGGTAAATTGATGCTACAAATTCTAGCGAGTTTTTCAG
AATTTGAAAGAAATAATATTGTCGAAAATGTATTCATGGGTCAAACCCGACGCGCTCAAGAAGGCTATTATCAAGGCAAT
TTGCCGCTGGGCTATGACAAAATACCGGATAGCAAGCATGAACTCATGATAAACCAACATGAAGCGAATATTGTCAAATA
TATATTTGAGTCATATGCTAAAGGCCACGGATATCGTAAAATTGCGAATGCACTCAATCACAAAGGATACGTGACTAAAA
AAGGAAAGCCTTTCAGTATTGGTTCAGTGACCTATATCTTATCTAATCCATTCTATGTTGGTAAAATTCAATTCGCAAAG
TACAAAGATTGGAATGAAAAGCGTCGTAAAGGGCTGAATGATAAACCAATAATAGCTGAAGGTAAGCATTCCCCTATTAT
TATTCAAGACTTATGGGATAAAGTCCAATTACGTAAAAAACAAGTCAGTCAAAAACCTCAAGTCCACGGTAAAGGAACTA
ATCTATTAACAGGTATCGTTCATTGTCCACAATGTGGTGCACCAATGGCAGCTAGTAACACAACGAACACATTGAAAGAT
GGTACCAAGAAGCGAATACGTTATTATTCTTGCAGTAACTTCCGAAACAAAGGCTCAAAAGTATGTTCTGCGAATAGCGT
TAGAGCTGATGTGATTGAAAAATACGTCATGGATCAAATACTCGAAATTGTCAAAAGTGATAAAGTCATTAACCAAGTCT
TAGAACGTGTCAATCAAGAAAATAAAGTCGATATTGGTGCATTGAACCACGATATCGCTTATAAACAACAACAATACGAT
GAAGTCAGCGGGAAACTCCATAATTTAGTTAAAACCATTGAAGATAATCCGGACCTAACATCTGCATTGAAAGCAACTAT
TCATCAATATGAAACACAACTCAATGACATTACAAATCAAATGAATCAACTCAAACAGCAACAAAATCAAGAGAAACTAT
CTTATGATACGAAACAAATCGCTGCCCTATTACAACGAATATTTCAAAATATAGAATCAATGGATAAAGCACAACTCAAA
GCATTATATCTTACAGTCATTGACCGTATTGATATTCGTAAAGACGGTAATCATAAAAAACAGTTCTACGTTACACTAAA
ACTCAATAATGAAATTATTAAACAACTTTTCAATAATACCCCTCTCGACGAAGTGCTCCTCAGCACTTCGTCTTTATTTT
TGCCTCAAACGCTCTTTCTTCAAATCTAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

86.347

100

0.863