Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   V6D45_RS00580 Genome accession   NZ_CP145722
Coordinates   105764..107140 (+) Length   458 a.a.
NCBI ID   WP_014112330.1    Uniprot ID   G4NQC1
Organism   Bacillus spizizenii strain kcgeb_S11     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100764..112140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6D45_RS00560 (V6D45_00560) ctsR 101117..101581 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  V6D45_RS00565 (V6D45_00565) mcsA 101595..102152 (+) 558 WP_338443620.1 protein-arginine kinase activator protein McsA -
  V6D45_RS00570 (V6D45_00570) - 102152..103243 (+) 1092 WP_061188087.1 protein arginine kinase -
  V6D45_RS00575 (V6D45_00575) clpC 103240..105672 (+) 2433 WP_003225731.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  V6D45_RS00580 (V6D45_00580) radA 105764..107140 (+) 1377 WP_014112330.1 DNA repair protein RadA Machinery gene
  V6D45_RS00585 (V6D45_00585) disA 107144..108226 (+) 1083 WP_014112331.1 DNA integrity scanning diadenylate cyclase DisA -
  V6D45_RS00590 (V6D45_00590) - 108341..109441 (+) 1101 WP_014112332.1 PIN/TRAM domain-containing protein -
  V6D45_RS00595 (V6D45_00595) ispD 109455..110153 (+) 699 WP_338443623.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  V6D45_RS00600 (V6D45_00600) ispF 110146..110622 (+) 477 WP_072182890.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49441.94 Da        Isoelectric Point: 7.9801

>NTDB_id=939644 V6D45_RS00580 WP_014112330.1 105764..107140(+) (radA) [Bacillus spizizenii strain kcgeb_S11]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSDSSNSVLYISGEESVKQTKLRADRLGINSPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFKDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=939644 V6D45_RS00580 WP_014112330.1 105764..107140(+) (radA) [Bacillus spizizenii strain kcgeb_S11]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGTTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACTATGGTGGAAGAAATGATAAAAAAAGCGCCGGCCAATCGGAGAGCGGCTTTTTCTCATTCAGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGGGTTAAAACCCAGCTTGGCGAGTTT
AACAGGGTGCTCGGCGGGGGCGTTGTCAAAGGCTCACTCGTTTTAATCGGCGGCGATCCTGGTATCGGAAAGTCAACGTT
ATTACTACAGGTTTCCGCTCAATTATCAGATTCATCGAACAGTGTGCTGTACATTTCGGGAGAAGAATCGGTAAAGCAAA
CGAAACTGAGAGCAGATCGTCTTGGCATTAATAGTCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTAGACTCTATTCAAACCGTTTACCAAAGTGACATTACATCGGC
TCCGGGCAGTGTGTCACAGGTCAGAGAATGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGCATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGATCTATTGCAGGGCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGGGAACGTCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTTGGTTCTACAAATGAAATGGGCATTTT
CGAAATGCGCGAAGAGGGGCTCACTGAGGTTTTGAACCCTTCAGAAATTTTCTTGGAAGAACGCTCAGCGGGATCCGCAG
GCTCAAGCATAACTGCTTCTATGGAGGGCACAAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAATCCGCGGCGTATGGCAACAGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCCGTGTTAGAAAAAAGAGTTGG
GCTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCCGGCGGCGTAAAGCTTGATGAACCTGCAATTGATCTTGCCA
TTGCGATCAGCATCGCGTCCAGCTTTAAAGATACACCTCCAAATCCTGCAGATTGTTTTATAGGAGAAGTGGGATTAACC
GGAGAAGTTCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCTAAGCTCGGTTTTAAACGAATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAGGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4NQC1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

98.908

100

0.989
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.355

98.908

0.627