Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   V5F86_RS05790 Genome accession   NZ_CP145137
Coordinates   1139849..1143553 (+) Length   1234 a.a.
NCBI ID   WP_338444087.1    Uniprot ID   -
Organism   Bacillus spizizenii strain kcgeb_sc     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1134849..1148553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5F86_RS05775 (V5F86_05780) - 1135295..1135624 (-) 330 WP_041520913.1 four-helix bundle copper-binding protein -
  V5F86_RS05780 (V5F86_05785) - 1135741..1136178 (-) 438 WP_014113300.1 ferritin family protein -
  V5F86_RS05785 (V5F86_05790) addB 1136362..1139862 (+) 3501 WP_338444086.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  V5F86_RS05790 (V5F86_05795) addA 1139849..1143553 (+) 3705 WP_338444087.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  V5F86_RS05795 (V5F86_05800) sbcD 1143625..1144800 (+) 1176 WP_268379045.1 exonuclease subunit SbcD -
  V5F86_RS05800 (V5F86_05805) sbcC 1144797..1148189 (+) 3393 WP_338444088.1 exonuclease subunit SbcC -
  V5F86_RS05805 (V5F86_05810) hlpB 1148203..1148505 (+) 303 WP_338444090.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141350.70 Da        Isoelectric Point: 5.0978

>NTDB_id=937397 V5F86_RS05790 WP_338444087.1 1139849..1143553(+) (addA) [Bacillus spizizenii strain kcgeb_sc]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLQRA
LELTKAPGGPAPRADNFLDDLGQLDELIQHQDDFTELYKRVPAVSFKRAKAVKGDEFDPALLEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAEDETGERQPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDKTETELLLIDNAEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLETFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDMEVLAENDAAMHADISGHPARFAVRMIHSYD
LLDDDLEERTEEKSERLEAIHKGEPVPGSFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHIPTIEEAEQTVHKLYEKELLTEEQKNAIDIEDIVQFFHTEIGG
QLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=937397 V5F86_RS05790 WP_338444087.1 1139849..1143553(+) (addA) [Bacillus spizizenii strain kcgeb_sc]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTCTCAACCGGCCAGGATATTCT
CGTGGCAGCGGCGGCGGGCTCCGGTAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATCACCGCGGAAGAAAACC
CGATTGATGTAGACCGGCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCC
CTGGAAAAAGAGCTTGTAAAGCGCCCCGGCTCGCTGCATATCAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
AACACTTCATTCCTTTTGCCTGCAAGTGCTGAAAAAATATTACTATTTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTGCTCGGGGATGAGGTGCTGGACGAGCTGTTTGAAGACGAATACGCAAAAGGCGAAAAGGCG
TTTTTTGAACTTGTTGACCGCTACACGACAGACCGACACGATCTGGATCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCACGTTCCCACCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCGGAAAAGAGCGCCATCG
AGGAGCTTCCGTTTTATCAATATGTCAAAGAGGATATTGCGATGGTGCTTAACGGAGCAAAGGAGAAGCTTCAGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCCGCGCCGCGCGCTGACAATTTTCTTGATGATCTTGGGCAGCTTGATGAACT
GATTCAGCATCAGGATGATTTCACTGAGCTTTATAAGCGGGTGCCAGCAGTCTCTTTTAAGCGCGCGAAAGCAGTAAAAG
GGGACGAGTTTGATCCTGCACTCCTTGAAGAAGCGACAGATTTGAGAAACGGTGCAAAAAAACTGCTTGAAAAGCTTAAA
ACCGACTACTTCACAAGAAGTCCTGAACAGCATTTGAAAAGCTTGGCCGACATGAAGCCTGTTATTGAAACGCTCGTACA
GCTTGTCATCAGCTATGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTTTCTGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCTGAGGATGAAACAGGTGAGCGCCAGCCGAGCGAGGCAGCAAGATTTTATCAAGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTAGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGACC
GGAGGAAACAGGCAACCTGTTTATGGTAGGTGATGTCAAACAGTCCATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCCAAATACAAACGGTTTACAGAGAGCGGAGAAGGCACAGGACGGAAAATTGACTTGAATAAAAATTTCCGAAGC
CGGGCTGATATTTTAGATAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTATCCGGACAATGATAAGACGGAAACGGAGCTGCTGCTGATCGACAACGCAG
AAGAGACGGATGCAGGCGAAGAAGCAGAAGAGCTGGAAACGGTGCAGTTCGAGGCGAAAGCGATCGCTAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTCCGCTCCATGCCGTGGGCGCCGCAAATCATGGAGGAGCTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCGGTCGAAGTTGCCGTCGCGCTTTCTGTGCTGAAGGTAATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTGCGCTCACCAATTGTCGGAGCGGATGAAAACGAGCTCTCTTTGATCCGGCTTGAAAATAAAAA
AGCGCCGTACTATGAAGCGATGAAAGACTATTTGGCTGCCGGCGACCGGAACGACGAGCTTTATCAAAAGCTGGAAACGT
TCTACGGACATCTTCAAAAATGGCGTGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTCTATGACCGTGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTCCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACGGCGCGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCCGTCGTGTTTGCAGCAGGTCTCGGCCGGAATTTCAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTTGGGTTCGGCACGAAGTATATTCATCCGCAATTGCGCATCAGCTACCCGACGCTGCCGCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAAGAAAAACTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAGAAACAGCTCGCAAAATGGCAGGCATCCGCGTCTCAAACTGACTGGCTTCTTCCGGA
ATTTGACCGTTATCAGGCAAAAACGTATTTAGATTTCATCGGACCGGCTCTTGCCAGACACAGAGATATGGAGGTTTTGG
CTGAAAACGATGCGGCAATGCATGCTGATATATCAGGACACCCGGCCCGTTTTGCCGTTCGAATGATTCATTCCTATGAT
TTGCTGGATGATGATCTGGAAGAAAGAACGGAAGAAAAAAGCGAGCGCTTAGAAGCGATCCACAAAGGTGAACCAGTTCC
CGGCTCGTTTGCATTTGATGAAAAAGCCCGTGAGCAGCTGAGCTGGACATACCCGCATCACGAGGTCACGCAAATTCGTA
CAAAACAGTCGGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCCGGGCCCCCGTTAAACCTGCT
GACGGAAGCATTCTGTACAGGCGTCCCGCTTTTATGATGAAAAAAGGCCTGACAGCGGCGGAGAAAGGGACGGCCATGCA
TACGGTTATGCAACATATCCCGCTTACCCATATCCCAACGATAGAAGAAGCGGAGCAGACGGTTCACAAGCTTTATGAAA
AAGAACTGCTCACTGAAGAACAAAAAAACGCTATTGATATAGAAGATATCGTGCAATTTTTCCATACAGAAATCGGCGGA
CAGCTGATCGGCGCTAAATGGAAGGACCGGGAAGTGCCATTTAGTTTAGCGCTTCCGGCCAAGGAGATCTATCCTGATGC
ACAGGAGGCGGATGAACCGCTTTTAGTGCAGGGAATCATCGACTGTCTCTATGAAACTGAGGACGGCTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCTTGAAGAAACGATATGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

96.515

100

0.965