Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   V5F86_RS05785 Genome accession   NZ_CP145137
Coordinates   1136362..1139862 (+) Length   1166 a.a.
NCBI ID   WP_338444086.1    Uniprot ID   -
Organism   Bacillus spizizenii strain kcgeb_sc     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1131362..1144862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5F86_RS05765 (V5F86_05770) - 1132216..1133418 (-) 1203 WP_338444082.1 MFS transporter -
  V5F86_RS05770 (V5F86_05775) - 1133532..1135259 (+) 1728 WP_338444084.1 ABC transporter substrate-binding protein -
  V5F86_RS05775 (V5F86_05780) - 1135295..1135624 (-) 330 WP_041520913.1 four-helix bundle copper-binding protein -
  V5F86_RS05780 (V5F86_05785) - 1135741..1136178 (-) 438 WP_014113300.1 ferritin family protein -
  V5F86_RS05785 (V5F86_05790) addB 1136362..1139862 (+) 3501 WP_338444086.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  V5F86_RS05790 (V5F86_05795) addA 1139849..1143553 (+) 3705 WP_338444087.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  V5F86_RS05795 (V5F86_05800) sbcD 1143625..1144800 (+) 1176 WP_268379045.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134729.78 Da        Isoelectric Point: 5.5268

>NTDB_id=937396 V5F86_RS05785 WP_338444086.1 1136362..1139862(+) (addB) [Bacillus spizizenii strain kcgeb_sc]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPFAEDIKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTQTPELAHLEAQYEARPAIPYAEKQEAFTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSIDVLKGNWRY
EAVFRCVKTELLFPLNEPKTKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDDFAKTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGKIIEAQQHQQAWDAVIQLLEEFVEMMGDEEI
SLDLFQQMMEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTVGIELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVIKRLEELFPQHKERLLTNEPEQVSDEEQLMYVVNKS
VAQSFTASQLRLWNREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKMDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=937396 V5F86_RS05785 WP_338444086.1 1136362..1139862(+) (addB) [Bacillus spizizenii strain kcgeb_sc]
TTGGGAGCAGAGTTTTTAGTAGGCAGATCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATATGGGCGGAATGATACGTGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGCGTCCTTCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTTACGAGCACAGGCGTACAGATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCAAGTGACAAAAGCGGATTTACCGCACAGGTTGAACGGATGCTGACAGAATTTAAGCGTTACT
GCCTGGAACCAGAAGATATCCGCCGGATGGCGGAAAGCGGAACGGCTTCTGAGTATCGCGGGGAACGTGTTTTATCTGAA
AAGCTTCATGATTTATCAATCCTGTATCAGCAGATGGAAAAAAGCCTTGCAGATCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGGCAGAGCATATTCCGTTTGCGGAAGATATAAAAGGCGCGCATATTTATGTGGACGGCTTTTATCAATTTA
CACCGCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCATGCGGAGCACATCACGTTTTCGCTCACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGAAAAACGTATTATCGCCTGCATCAGAAGGCGAA
GGAACTGAATCTGGACATTACCTATAAAGAGCTAAGCGGGACTGAACGGCATACGCAGACGCCGGAACTGGCACATCTAG
AGGCGCAGTATGAAGCGCGACCGGCCATCCCGTACGCAGAAAAACAAGAGGCCTTTACTGTGATGCAGGCCGCAAACAGA
CGAGCTGAACTGGAAGGCATTGCCCGGGAAATTCACGCTCTTGTCAGAGAGAAGGGCTATCGCTATAAGGATGTAGCGAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCTTCTATGCTGAACCATCCGTTAATCGAATTTATCCGGTCGAGCATTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTTAACGAGCCGAAAACTAAGGTAAGAGAACAGGTCGA
TCAGCTTGAAAATTACTGTATCGCCTACGGGATCAAAGGCGATCGCTGGACAAAGGGCGACCGTTTCCATTACAGACGTT
TTGTGTCATTGGATGATGATTTTGCGAAAACCGATCAGGAAATCGAAATGGAAAACATGCTGAATGACACACGCGACTGG
ATTGTTCCGCCGCTTTTTCAGCTTCAAAAACGAATGAAAAAAGCAAAGACGGTTCAGGAGAAGGCGGAGGCGCTCTACCG
CTATCTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGTGCTGAGGAAGACGGCAAAATTATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGAGATGAAGAGATT
TCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGTTGGGGGCAAACGACGGCGTTC
TGCCTGCACGCCCCGATGAAAATGGGGTGCTGTCGGATGATGACCGGGAATGGCTGAAAACGGTTGGGATTGAACTTTCC
TCAGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGTTTTCCAGTCCGTCTGACCGGCTTTACGT
ATCGTATCCGATTGCCGATGCGGAAGGAAAAACGCTTTTGCCATCGATTGTCATTAAACGGCTGGAAGAACTGTTTCCGC
AGCATAAGGAGCGTCTGTTGACAAATGAACCTGAACAGGTCAGCGATGAGGAACAGCTTATGTATGTCGTCAATAAAAGC
GTGGCACAGTCCTTTACCGCGAGCCAGCTCAGATTATGGAATCGGGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCCGGACAGGCTGCAATCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTTTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCG
TGCCCGTTTTCCCATTTCGCATCACACGGGCTGCATCTAAAGGAACGGCAATTTTTCAAGCTCGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACCGGCTGCGCGAGCAAAAAATGGATTGGCGCGATTTAACGAAAG
AGCAGTGCGAGCTGTTTTCCTATGATGCGGTAGAGCGGCTGGCGCCGAAACTGCAAAAGGAAATTTTGCTCAGCTCAAAC
CGGCATTATTATGTAAAGGAAAAACTGCAAAAAATTGTGACACGCGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTTGTGCCGATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTAAGCTGAAAAACG
GCTGCACGATGGAACTCGTCGGGCGAATTGACCGCGTAGATAAAGCGGAAAGCTCAAAAGGCCTGCTCCTAAGGATTGTT
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGACTGGCGCTGCAAATGCTGACGTATCTTGA
TTTATCAATTACTCATTCAGCTGACTGGCTCGGAATGAGGGCGACGCCTGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAAGGCTTG
CTCCTCGGCGATCAGGAAGCGGTTCGCCTCATGGATACGACCCTTCAAGAGGGACGGTCGAATATCATCAACGCGGGCTT
GAAGAAAGACGGCTCTCTCAGATCGGATTCAGCGGCAGTCGGCGAGAAGGAATTTGAGCTGTTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAACAAATCACTGACGGACGCGTATCGATTGAGCCGTATAAAATGAAGAACAAGACGCCG
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAGTCATTAGAAGAAAACGAGTATCGCCCGTTAAAGGCTGA
AAAGGATAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

97.942

100

0.979