Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LLL8_RS08925 Genome accession   NZ_AP025700
Coordinates   1833401..1834048 (-) Length   215 a.a.
NCBI ID   WP_058203544.1    Uniprot ID   A0AB35KBC8
Organism   Lactococcus lactis strain L8     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1828401..1839048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLL8_RS08900 (LLL8_17450) atpF 1828470..1828976 (-) 507 WP_010906128.1 F0F1 ATP synthase subunit B -
  LLL8_RS08905 (LLL8_17460) atpB 1828991..1829704 (-) 714 WP_004255255.1 F0F1 ATP synthase subunit A -
  LLL8_RS08910 (LLL8_17470) - 1829749..1829964 (-) 216 WP_004255250.1 F0F1 ATP synthase subunit C -
  LLL8_RS08915 (LLL8_17480) - 1830149..1830925 (-) 777 WP_058220836.1 alpha/beta hydrolase family protein -
  LLL8_RS08920 (LLL8_17490) comEC 1831210..1833420 (-) 2211 WP_251899790.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LLL8_RS08925 (LLL8_17500) comEA 1833401..1834048 (-) 648 WP_058203544.1 ComEA family DNA-binding protein Machinery gene
  LLL8_RS08930 (LLL8_17510) - 1834108..1835469 (-) 1362 WP_228248326.1 ABC transporter permease -
  LLL8_RS08935 (LLL8_17520) - 1835466..1836398 (-) 933 WP_004255238.1 ABC transporter ATP-binding protein -
  LLL8_RS08940 (LLL8_17530) - 1836503..1836901 (-) 399 WP_004255236.1 hypothetical protein -
  LLL8_RS08945 (LLL8_17540) - 1837005..1837568 (-) 564 WP_004255233.1 GNAT family N-acetyltransferase -
  LLL8_RS08950 (LLL8_17550) - 1837740..1838918 (-) 1179 WP_251899791.1 SLC13 family permease -

Sequence

Protein


Download         Length: 215 a.a.        Molecular weight: 23634.80 Da        Isoelectric Point: 4.8659

>NTDB_id=93700 LLL8_RS08925 WP_058203544.1 1833401..1834048(-) (comEA) [Lactococcus lactis strain L8]
MDKILEKVKEYWKMIVLIVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISPDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
VTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide


Download         Length: 648 bp        

>NTDB_id=93700 LLL8_RS08925 WP_058203544.1 1833401..1834048(-) (comEA) [Lactococcus lactis strain L8]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAATTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGCCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
GTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATTGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(151-212)

SLBB domain (SLBB)

(70-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

74.771

100

0.758
  comEA/celA/cilE Streptococcus mitis SK321

44.444

100

0.447
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

43.119

100

0.437
  comEA/celA/cilE Streptococcus pneumoniae Rx1

41.096

100

0.419
  comEA/celA/cilE Streptococcus pneumoniae D39

41.096

100

0.419
  comEA/celA/cilE Streptococcus pneumoniae R6

41.096

100

0.419
  comEA/celA/cilE Streptococcus mitis NCTC 12261

41.284

100

0.419
  comEA Latilactobacillus sakei subsp. sakei 23K

36.123

100

0.381

Multiple sequence alignment