Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   V5F99_RS10560 Genome accession   NZ_CP145053
Coordinates   2022105..2022500 (-) Length   131 a.a.
NCBI ID   WP_353165085.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain WHO_beta_2024     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2017105..2027500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5F99_RS10540 (V5F99_10570) - 2017292..2018233 (-) 942 WP_003686969.1 ABC transporter ATP-binding protein -
  V5F99_RS10545 (V5F99_10575) - 2018242..2019117 (-) 876 WP_003686971.1 NUDIX hydrolase family protein -
  V5F99_RS10555 (V5F99_10585) thiC 2019715..2021616 (+) 1902 WP_082295455.1 phosphomethylpyrimidine synthase ThiC -
  V5F99_RS10560 (V5F99_10590) pilE 2022105..2022500 (-) 396 WP_353165085.1 pilin Machinery gene
  V5F99_RS10565 (V5F99_10595) - 2022852..2023250 (-) 399 Protein_2041 pilin -
  V5F99_RS10570 (V5F99_10600) - 2023412..2023744 (-) 333 Protein_2042 pilin -
  V5F99_RS10575 (V5F99_10605) - 2023763..2024154 (-) 392 Protein_2043 pilin -
  V5F99_RS10580 (V5F99_10610) - 2024764..2025123 (-) 360 Protein_2044 pilin -
  V5F99_RS10585 (V5F99_10615) - 2025164..2025562 (-) 399 Protein_2045 pilin -
  V5F99_RS10590 (V5F99_10620) - 2026100..2026453 (-) 354 Protein_2046 pilin -
  V5F99_RS10595 (V5F99_10625) - 2026506..2026934 (-) 429 WP_017147177.1 pilin -
  V5F99_RS10600 (V5F99_10630) - 2026986..2027294 (+) 309 Protein_2048 NAD(P)-dependent oxidoreductase -

Sequence

Protein


Download         Length: 131 a.a.        Molecular weight: 14071.83 Da        Isoelectric Point: 10.0251

>NTDB_id=936582 V5F99_RS10560 WP_353165085.1 2022105..2022500(-) (pilE) [Neisseria gonorrhoeae strain WHO_beta_2024]
MPAGILKHRPKKSAVAGYCLNHGIWPKDNTSAGVASSSSIKGKYVKEVKVENGVVTATMNSSGVNKEIQGKRLSLWGRRE
NGSVKWFCGQPVKRAKAANDDAVTDDTTGNGNEKIETKHLPSTCRDESSAE

Nucleotide


Download         Length: 396 bp        

>NTDB_id=936582 V5F99_RS10560 WP_353165085.1 2022105..2022500(-) (pilE) [Neisseria gonorrhoeae strain WHO_beta_2024]
TTGCCCGCCGGAATATTAAAACACCGCCCAAAAAAATCAGCCGTTGCCGGGTATTGCCTGAATCACGGCATATGGCCGAA
AGACAACACTTCTGCCGGCGTGGCATCTTCTTCATCAATCAAAGGCAAATATGTTAAGGAAGTTAAAGTCGAAAACGGCG
TCGTCACCGCCACAATGAATTCAAGCGGCGTAAACAAAGAAATCCAAGGCAAAAGACTCTCCCTGTGGGGCAGGCGTGAA
AACGGTTCGGTAAAATGGTTCTGCGGACAGCCGGTTAAGCGCGCCAAAGCCGCCAACGACGACGCCGTCACCGACGACAC
CACCGGCAACGGCAACGAAAAAATCGAAACCAAGCACCTGCCGTCAACCTGCCGCGATGAATCATCTGCCGAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(4-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

68.504

96.947

0.664
  pilE Neisseria gonorrhoeae strain FA1090

66.929

96.947

0.649