Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   SAJP025_RS08000 Genome accession   NZ_AP025683
Coordinates   1666059..1666745 (-) Length   228 a.a.
NCBI ID   WP_001794049.1    Uniprot ID   A0A0E1X4U4
Organism   Staphylococcus aureus strain JP025     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1661059..1671745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAJP025_RS07980 (SAJP025_15240) rpsT 1661973..1662224 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  SAJP025_RS07985 (SAJP025_15250) holA 1662269..1663243 (-) 975 WP_001282571.1 DNA polymerase III subunit delta -
  SAJP025_RS07990 (SAJP025_15260) comEC 1663300..1665501 (-) 2202 WP_000967506.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SAJP025_RS07995 (SAJP025_15270) - 1665506..1665967 (-) 462 WP_000439692.1 ComE operon protein 2 -
  SAJP025_RS08000 (SAJP025_15280) comEA 1666059..1666745 (-) 687 WP_001794049.1 ComEA family DNA-binding protein Machinery gene
  SAJP025_RS08005 (SAJP025_15290) - 1666785..1667501 (-) 717 WP_000084835.1 class I SAM-dependent methyltransferase -
  SAJP025_RS08010 (SAJP025_15300) rsfS 1667504..1667857 (-) 354 WP_001088020.1 ribosome silencing factor -
  SAJP025_RS08015 (SAJP025_15310) yqeK 1667858..1668442 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  SAJP025_RS08020 (SAJP025_15320) nadD 1668432..1669001 (-) 570 WP_000822938.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  SAJP025_RS08025 (SAJP025_15330) yhbY 1669004..1669294 (-) 291 WP_000955236.1 ribosome assembly RNA-binding protein YhbY -
  SAJP025_RS08030 (SAJP025_15340) aroE 1669298..1670104 (-) 807 WP_000666750.1 shikimate dehydrogenase -
  SAJP025_RS08035 (SAJP025_15350) yqeH 1670118..1671218 (-) 1101 WP_001280133.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25766.38 Da        Isoelectric Point: 9.3383

>NTDB_id=93586 SAJP025_RS08000 WP_001794049.1 1666059..1666745(-) (comEA) [Staphylococcus aureus strain JP025]
MVLLYQFLLRYKDFLAQWKLYIISAVVLIMVLIGFIFWRQDDYTSRDFENKDTVLKQSTSENSSLSKLEDVQVKDGDNSK
NKGPVYVDIKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGANSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLNSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=93586 SAJP025_RS08000 WP_001794049.1 1666059..1666745(-) (comEA) [Staphylococcus aureus strain JP025]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAGCTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAGATTTTGAAAATAAAGATACTG
TTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATATAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTAGATGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATTCCTCATAAAGGACAAAAGAATGTTGAACCACAATTTGGAGCAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAATTCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E1X4U4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

95.595

99.561

0.952
  comEA Staphylococcus aureus N315

95.595

99.561

0.952

Multiple sequence alignment