Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   MWM15_RS02200 Genome accession   NZ_AP025675
Coordinates   456558..457682 (+) Length   374 a.a.
NCBI ID   WP_000228517.1    Uniprot ID   E2QFE2
Organism   Escherichia coli strain Rb-3     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 451558..462682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM15_RS02175 (TRECRb3_04310) mscL 451675..452088 (-) 414 WP_000022450.1 large-conductance mechanosensitive channel protein MscL -
  MWM15_RS02180 (TRECRb3_04320) trkA 452218..453594 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  MWM15_RS02185 (TRECRb3_04330) rsmB 453616..454905 (-) 1290 WP_000744767.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  MWM15_RS02190 (TRECRb3_04340) fmt 454957..455904 (-) 948 WP_000004426.1 methionyl-tRNA formyltransferase -
  MWM15_RS02195 (TRECRb3_04350) def 455919..456428 (-) 510 WP_000114984.1 peptide deformylase -
  MWM15_RS02200 (TRECRb3_04360) dprA 456558..457682 (+) 1125 WP_000228517.1 DNA-protecting protein DprA Machinery gene
  MWM15_RS02205 (TRECRb3_04370) smg 457654..458127 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  MWM15_RS02210 yrdD 458156..458698 (+) 543 WP_001129723.1 type I DNA topoisomerase -
  MWM15_RS02215 (TRECRb3_04390) tsaC 458703..459275 (+) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  MWM15_RS02220 (TRECRb3_04400) aroE 459280..460098 (+) 819 WP_000451224.1 shikimate dehydrogenase -
  MWM15_RS02225 (TRECRb3_04410) yrdB 460095..460352 (+) 258 WP_001070580.1 DUF1488 domain-containing protein -
  MWM15_RS02230 (TRECRb3_04420) yrdA 460328..460882 (-) 555 WP_001541769.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40910.86 Da        Isoelectric Point: 6.0395

>NTDB_id=93494 MWM15_RS02200 WP_000228517.1 456558..457682(+) (dprA) [Escherichia coli strain Rb-3]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=93494 MWM15_RS02200 WP_000228517.1 456558..457682(+) (dprA) [Escherichia coli strain Rb-3]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT
TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGCCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E2QFE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

44.214

90.107

0.398
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39

Multiple sequence alignment