Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   V4943_RS05190 Genome accession   NZ_CP144685
Coordinates   992809..993942 (+) Length   377 a.a.
NCBI ID   WP_011173432.1    Uniprot ID   -
Organism   Thermus thermophilus strain HB27_delta_csm3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 987809..998942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4943_RS05150 (V4943_05150) - 987810..988568 (+) 759 WP_011173427.1 type III pantothenate kinase -
  V4943_RS05155 (V4943_05155) - 988531..988896 (-) 366 WP_024119691.1 hypothetical protein -
  V4943_RS05170 (V4943_05170) - 989591..989875 (-) 285 WP_226980268.1 hypothetical protein -
  V4943_RS05175 (V4943_05175) - 989757..991073 (+) 1317 WP_338457458.1 HD domain-containing phosphohydrolase -
  V4943_RS05180 (V4943_05180) - 991124..991615 (+) 492 WP_011173430.1 DUF5317 domain-containing protein -
  V4943_RS05185 (V4943_05185) - 991670..992674 (+) 1005 WP_011173431.1 homoisocitrate dehydrogenase -
  V4943_RS05190 (V4943_05190) pilM 992809..993942 (+) 1134 WP_011173432.1 type IV pilus assembly protein PilM Machinery gene
  V4943_RS05195 (V4943_05195) pilN 993935..994558 (+) 624 WP_011173433.1 competence protein Machinery gene
  V4943_RS05200 (V4943_05200) pilO 994548..995129 (+) 582 WP_011173434.1 type 4a pilus biogenesis protein PilO Machinery gene
  V4943_RS05205 (V4943_05205) pilW 995126..996004 (+) 879 WP_011173435.1 hypothetical protein Machinery gene
  V4943_RS05210 (V4943_05210) pilQ 996001..998274 (+) 2274 WP_011173436.1 secretin N-terminal domain-containing protein Machinery gene

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41399.02 Da        Isoelectric Point: 4.3805

>NTDB_id=934419 V4943_RS05190 WP_011173432.1 992809..993942(+) (pilM) [Thermus thermophilus strain HB27_delta_csm3]
MFKSLSQLFRPRVEALGLEIGASALKLVEVSGNPPALKALASRPTPPGLLMEGMVAEPAALAQEIKELLLEARTRKRYVV
TALSNLAVILRPIQVPKMPLKEMEEAVRWEAERYIPFPIDEVVLDFAPLTPLSEVQEGEQVQVMVAAARQEAVAGVLEAL
RGAGLVPVVLDVKPFAGLYPLEARLAEEPDRVFLVLDIGAESTSLVLLRGDKPLAVRVLTLSGKDFTEAIARSFNLDLLA
AEEVKRTYGMATLPTEDEELLLDFDAERERYSPGRIYDAIRPVLVELTQELRRSLEFFRIQLEEASPEMGYLLGGGSKLR
GLASLLTDTLGVNFEPVNPWEAVAVDPKRFESEQLQEIGPEFAVALGLALRGVEPLD

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=934419 V4943_RS05190 WP_011173432.1 992809..993942(+) (pilM) [Thermus thermophilus strain HB27_delta_csm3]
GTGTTCAAAAGCCTTAGCCAGCTCTTCCGACCCAGGGTTGAGGCCCTGGGCTTGGAGATCGGGGCCTCCGCCCTGAAGCT
CGTGGAGGTGTCCGGGAACCCCCCTGCCCTCAAAGCCCTGGCCTCCCGCCCCACCCCGCCCGGCCTCCTGATGGAGGGAA
TGGTGGCCGAGCCTGCCGCGCTGGCCCAGGAGATCAAGGAGCTTCTCCTCGAGGCCCGCACCCGCAAGCGCTACGTGGTC
ACCGCCCTCTCCAACCTGGCGGTGATCCTCCGCCCCATCCAGGTTCCCAAGATGCCCCTCAAGGAGATGGAGGAGGCGGT
GCGCTGGGAGGCGGAGCGCTACATTCCCTTCCCCATTGACGAGGTGGTCCTGGACTTCGCCCCCCTGACCCCCCTCTCCG
AGGTGCAGGAGGGGGAGCAGGTCCAGGTGATGGTGGCGGCGGCGCGGCAGGAGGCGGTGGCGGGGGTCCTCGAGGCCCTG
AGGGGGGCGGGGCTCGTCCCCGTGGTGCTGGACGTGAAGCCCTTCGCCGGGCTTTACCCTCTGGAGGCCAGGCTTGCGGA
GGAGCCCGACCGGGTCTTCCTCGTCCTGGACATCGGGGCCGAGAGCACGAGCCTCGTCCTGCTCCGGGGGGACAAGCCCC
TGGCGGTGCGCGTCCTCACCCTCTCGGGCAAGGACTTCACCGAGGCCATCGCCCGAAGCTTCAACCTGGACCTCCTCGCC
GCCGAGGAGGTGAAGCGGACCTACGGGATGGCCACCCTCCCCACCGAGGACGAGGAGCTCCTCCTGGACTTTGACGCCGA
GCGGGAGCGCTACAGCCCGGGGCGCATCTACGACGCCATCCGCCCGGTGCTGGTGGAGCTCACCCAGGAGCTCCGCCGTA
GCCTGGAGTTCTTCCGCATCCAGCTGGAGGAGGCCTCGCCCGAGATGGGCTACCTCCTGGGCGGGGGGAGCAAGCTCAGG
GGGCTCGCCTCCCTCCTCACCGACACCCTGGGGGTGAACTTTGAGCCCGTCAACCCCTGGGAGGCGGTGGCGGTGGACCC
CAAGCGCTTTGAGTCGGAGCAACTCCAGGAGATCGGGCCGGAGTTCGCCGTGGCCCTGGGCTTGGCGTTGAGGGGGGTGG
AGCCCCTTGATTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-372)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Thermus thermophilus HB27

100

100

1