Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   V3I11_RS08015 Genome accession   NZ_CP144280
Coordinates   1591891..1592430 (+) Length   179 a.a.
NCBI ID   WP_005649140.1    Uniprot ID   -
Organism   Haemophilus influenzae strain P3_EMO     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1586891..1597430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V3I11_RS07995 (V3I11_07995) - 1587344..1588264 (-) 921 WP_005630123.1 ATP-binding cassette domain-containing protein -
  V3I11_RS08000 (V3I11_08000) - 1588264..1589145 (-) 882 WP_015701596.1 metal ABC transporter substrate-binding protein -
  V3I11_RS08005 (V3I11_08005) - 1589515..1590393 (-) 879 WP_042599462.1 D-alanyl-D-alanine carboxypeptidase family protein -
  V3I11_RS08010 (V3I11_08010) - 1590640..1591791 (+) 1152 WP_042599461.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  V3I11_RS08015 (V3I11_08015) pilF/pilF2 1591891..1592430 (+) 540 WP_005649140.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  V3I11_RS08020 (V3I11_08020) - 1592502..1593413 (+) 912 WP_042599460.1 helix-turn-helix domain-containing protein -
  V3I11_RS08025 (V3I11_08025) ispG 1593422..1594528 (+) 1107 WP_021035647.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  V3I11_RS08030 (V3I11_08030) hisS 1594538..1595809 (+) 1272 WP_021035648.1 histidine--tRNA ligase -
  V3I11_RS08035 (V3I11_08035) - 1595827..1596441 (+) 615 WP_042599459.1 YfgM family protein -
  V3I11_RS08040 (V3I11_08040) iscX 1596492..1596686 (-) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  V3I11_RS08045 (V3I11_08045) fdx 1596686..1597027 (-) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20555.22 Da        Isoelectric Point: 8.6194

>NTDB_id=933311 V3I11_RS08015 WP_005649140.1 1591891..1592430(+) (pilF/pilF2) [Haemophilus influenzae strain P3_EMO]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=933311 V3I11_RS08015 WP_005649140.1 1591891..1592430(+) (pilF/pilF2) [Haemophilus influenzae strain P3_EMO]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCGTCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTACATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

96.648

100

0.966
  pilF Glaesserella parasuis strain SC1401

45.81

100

0.458