Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   VXF94_RS09140 Genome accession   NZ_CP143956
Coordinates   1813167..1813604 (+) Length   145 a.a.
NCBI ID   WP_013350968.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Fad 108     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1808167..1818604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VXF94_RS09125 (VXF94_09125) - 1811033..1811395 (-) 363 WP_013350971.1 winged helix-turn-helix transcriptional regulator -
  VXF94_RS09130 (VXF94_09130) hxlA 1811627..1812262 (+) 636 WP_013350970.1 3-hexulose-6-phosphate synthase -
  VXF94_RS09135 (VXF94_09135) hxlB 1812259..1812816 (+) 558 WP_013350969.1 6-phospho-3-hexuloisomerase -
  VXF94_RS09140 (VXF94_09140) nucA/comI 1813167..1813604 (+) 438 WP_013350968.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  VXF94_RS09145 (VXF94_09145) nin/comJ 1813625..1814023 (+) 399 WP_013350967.1 competence protein ComJ Regulator
  VXF94_RS09150 (VXF94_09150) - 1814064..1815500 (-) 1437 WP_013350966.1 glycoside hydrolase family 1 protein -
  VXF94_RS09155 (VXF94_09155) - 1815605..1815928 (-) 324 WP_013350965.1 YckD family protein -
  VXF94_RS09160 (VXF94_09160) - 1816002..1816436 (-) 435 WP_013350964.1 RDD family protein -
  VXF94_RS09165 (VXF94_09165) - 1816451..1817587 (-) 1137 WP_041915596.1 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=932203 VXF94_RS09140 WP_013350968.1 1813167..1813604(+) (nucA/comI) [Bacillus amyloliquefaciens strain Fad 108]
MKTLKALLLVLIIIAGAVAALIKGDIFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=932203 VXF94_RS09140 WP_013350968.1 1813167..1813604(+) (nucA/comI) [Bacillus amyloliquefaciens strain Fad 108]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATATTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAACAATCTCTTAAACATGTGCCTGTAAAAACAGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCAGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641