Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V1582_RS00280 Genome accession   NZ_CP143688
Coordinates   48319..49752 (+) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. xiangfangensis strain ECC-182     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 43319..54752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1582_RS00250 (V1582_00250) - 43886..44863 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  V1582_RS00255 (V1582_00255) kdsD 44877..45863 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  V1582_RS00260 (V1582_00260) kdsC 45878..46444 (+) 567 WP_003861856.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  V1582_RS00265 (V1582_00265) lptC 46441..47016 (+) 576 WP_059294315.1 LPS export ABC transporter periplasmic protein LptC -
  V1582_RS00270 (V1582_00270) lptA 46985..47539 (+) 555 WP_003861860.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  V1582_RS00275 (V1582_00275) lptB 47546..48271 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  V1582_RS00280 (V1582_00280) rpoN/rpoN1 48319..49752 (+) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  V1582_RS00285 (V1582_00285) hpf 49775..50062 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  V1582_RS00290 (V1582_00290) ptsN 50145..50636 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V1582_RS00295 (V1582_00295) rapZ 50682..51536 (+) 855 WP_003861870.1 RNase adapter RapZ -
  V1582_RS00300 (V1582_00300) npr 51533..51805 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  V1582_RS00305 (V1582_00305) mtgA 51845..52570 (-) 726 WP_003861874.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  V1582_RS00310 (V1582_00310) elbB 52567..53220 (-) 654 WP_023314910.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=930659 V1582_RS00280 WP_003861864.1 48319..49752(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. xiangfangensis strain ECC-182]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=930659 V1582_RS00280 WP_003861864.1 48319..49752(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. xiangfangensis strain ECC-182]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTCCAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAGACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCACTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACTACCCAATCGCTTCAGGACTACCTGATGTGGCAGGTGGAGCTGACGCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGTGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCTAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAACCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCACTCGATCCGCGTCCGGGGCAGTCGATTCAGACCAG
CGAACCGGAATATGTCATTCCCGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACAAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATTAAGAGTCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATAGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCCATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48