Detailed information NTG0930225

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QAB28_RS02420
• Genome accession: NZ_CP143645
• Coordinates: 512804..513952 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain CPO100
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 507804..518952

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QAB28_RS02395 (QAB28_002395)	-	508955..509251 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
QAB28_RS02400 (QAB28_002400)	-	509248..509841 (-)	594	WP_003084544.1	YggT family protein	-
QAB28_RS02405 (QAB28_002405)	proC	509852..510673 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
QAB28_RS02410 (QAB28_002410)	-	510685..511377 (-)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
QAB28_RS02415 (QAB28_002415)	pilT	511592..512626 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QAB28_RS02420 (QAB28_002420)	pilU	512804..513952 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
QAB28_RS02425 (QAB28_002425)	czcD	513960..514859 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QAB28_RS02430 (QAB28_002430)	-	515013..515417 (+)	405	WP_003084560.1	NINE protein	-
QAB28_RS02435 (QAB28_002435)	-	515660..517033 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
QAB28_RS02440 (QAB28_002440)	-	517030..518214 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=930225 QAB28_RS02420 WP_003100959.1 512804..513952(+) (pilU) [Pseudomonas aeruginosa strain CPO100]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=930225 QAB28_RS02420 WP_003100959.1 512804..513952(+) (pilU) [Pseudomonas aeruginosa strain CPO100]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382