Detailed information NTG0093014

Bioinformatically predicted

Overview

• Name: pilA/pilA3
• Type: Machinery gene
• Locus tag: MWM13_RS06835
• Genome accession: NZ_AP025609
• Coordinates: 1280091..1280792 (+)
• Length: 233 a.a.
• NCBI ID: WP_244347491.1
• Uniprot ID: -
• Organism: Thermus thermophilus strain SNM3-3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1275091..1285792

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MWM13_RS06810 (TthSNM33_13490)	ilvN	1275504..1276022 (-)	519	WP_244347488.1	acetolactate synthase small subunit	-
MWM13_RS06815 (TthSNM33_13500)	ilvB	1276019..1277707 (-)	1689	WP_244347489.1	biosynthetic-type acetolactate synthase large subunit	-
MWM13_RS06820 (TthSNM33_13510)	-	1277863..1278843 (-)	981	WP_244340523.1	YpdA family putative bacillithiol disulfide reductase	-
MWM13_RS06825 (TthSNM33_13520)	pilA/pilA1	1279009..1279479 (+)	471	WP_244347490.1	GspH/FimT family protein	Machinery gene
MWM13_RS06830 (TthSNM33_13530)	pilA/pilA2	1279513..1280094 (+)	582	WP_011173284.1	type II secretion system protein	Machinery gene
MWM13_RS06835 (TthSNM33_13540)	pilA/pilA3	1280091..1280792 (+)	702	WP_244347491.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
MWM13_RS06840 (TthSNM33_13550)	comZ	1280803..1282479 (+)	1677	WP_244347492.1	pilus assembly PilX N-terminal domain-containing protein	Machinery gene
MWM13_RS06845 (TthSNM33_13560)	-	1282712..1283077 (+)	366	WP_244347493.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
MWM13_RS06850 (TthSNM33_13570)	-	1283137..1283508 (+)	372	WP_244347494.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
MWM13_RS06855 (TthSNM33_13590)	-	1283695..1284174 (+)	480	WP_244340535.1	DUF1640 domain-containing protein	-
MWM13_RS06860	-	1284250..1284787 (+)	538	Protein_1334	Uma2 family endonuclease	-
MWM13_RS06865 (TthSNM33_13610)	-	1284810..1285109 (+)	300	WP_008631315.1	nucleotidyltransferase	-
MWM13_RS06870 (TthSNM33_13620)	-	1285096..1285458 (+)	363	WP_060384345.1	HepT-like ribonuclease domain-containing protein	-

Sequence

Protein

• Length: 233 a.a.
• Molecular weight: 24960.43 Da
• Isoelectric Point: 6.2920

>NTDB_id=93014 MWM13_RS06835 WP_244347491.1 1280091..1280792(+) (pilA/pilA3) [Thermus thermophilus strain SNM3-3]
MKRGFTLVEVLVAMAILVVVLAVGVRYFASTSELARNTQARSELQDRVRMVMQVVTADLQMAGARYWNSGNQNQAFSLPL
PPLSGSDMGPKDTLTLYYVTSLRDLASACRRVDYGFEGDTLRRSDVNATPSSGSDCTTPPPNSQPLAEGMLALDIQYQCS
DGSQKDTPDCGTDAYPRSAKVTVAGYSLTPVTNPGPASLTTVTGKTLACPQGRACYALTQEVLMPNLKPLPTP

Nucleotide

• Length: 702 bp

>NTDB_id=93014 MWM13_RS06835 WP_244347491.1 1280091..1280792(+) (pilA/pilA3) [Thermus thermophilus strain SNM3-3]
ATGAAGCGCGGGTTCACCCTTGTGGAGGTCCTGGTGGCCATGGCCATCTTGGTGGTGGTCCTGGCCGTGGGCGTGCGCTA
CTTCGCAAGCACCAGCGAGCTCGCCCGGAACACTCAGGCAAGGAGCGAGCTCCAGGACCGGGTGCGCATGGTGATGCAGG
TGGTGACCGCCGACCTGCAGATGGCGGGGGCCCGCTACTGGAACAGCGGCAACCAAAACCAAGCCTTCAGCCTCCCCCTT
CCCCCGCTTTCGGGAAGCGACATGGGCCCTAAGGACACCCTAACCCTCTACTACGTCACGAGCCTCCGGGACCTGGCCTC
CGCCTGCCGCCGGGTGGACTACGGGTTTGAGGGGGACACCCTACGCCGGAGCGACGTGAACGCCACCCCCTCTTCGGGAA
GCGATTGCACCACCCCCCCTCCGAACTCGCAGCCCTTGGCCGAAGGGATGCTGGCCCTGGACATCCAATACCAGTGCAGC
GACGGAAGCCAGAAGGACACCCCCGACTGCGGTACAGACGCCTACCCCCGCTCGGCCAAGGTCACGGTGGCGGGCTACTC
CCTGACCCCGGTGACCAACCCGGGCCCCGCCTCCCTCACCACCGTCACCGGAAAGACCCTCGCCTGCCCCCAGGGCCGGG
CCTGCTACGCCCTCACCCAGGAGGTCCTCATGCCCAACCTCAAGCCCCTGCCCACCCCCTAG

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (2-23)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA/pilA3	Thermus thermophilus HB27	98.712	100	0.987