Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA2   Type   Machinery gene
Locus tag   MWM13_RS06830 Genome accession   NZ_AP025609
Coordinates   1279513..1280094 (+) Length   193 a.a.
NCBI ID   WP_011173284.1    Uniprot ID   Q72JC3
Organism   Thermus thermophilus strain SNM3-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1274513..1285094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM13_RS06810 (TthSNM33_13490) ilvN 1275504..1276022 (-) 519 WP_244347488.1 acetolactate synthase small subunit -
  MWM13_RS06815 (TthSNM33_13500) ilvB 1276019..1277707 (-) 1689 WP_244347489.1 biosynthetic-type acetolactate synthase large subunit -
  MWM13_RS06820 (TthSNM33_13510) - 1277863..1278843 (-) 981 WP_244340523.1 YpdA family putative bacillithiol disulfide reductase -
  MWM13_RS06825 (TthSNM33_13520) pilA/pilA1 1279009..1279479 (+) 471 WP_244347490.1 GspH/FimT family protein Machinery gene
  MWM13_RS06830 (TthSNM33_13530) pilA/pilA2 1279513..1280094 (+) 582 WP_011173284.1 type II secretion system protein Machinery gene
  MWM13_RS06835 (TthSNM33_13540) pilA/pilA3 1280091..1280792 (+) 702 WP_244347491.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MWM13_RS06840 (TthSNM33_13550) comZ 1280803..1282479 (+) 1677 WP_244347492.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  MWM13_RS06845 (TthSNM33_13560) - 1282712..1283077 (+) 366 WP_244347493.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MWM13_RS06850 (TthSNM33_13570) - 1283137..1283508 (+) 372 WP_244347494.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MWM13_RS06855 (TthSNM33_13590) - 1283695..1284174 (+) 480 WP_244340535.1 DUF1640 domain-containing protein -
  MWM13_RS06860 - 1284250..1284787 (+) 538 Protein_1334 Uma2 family endonuclease -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 20563.65 Da        Isoelectric Point: 9.1543

>NTDB_id=93013 MWM13_RS06830 WP_011173284.1 1279513..1280094(+) (pilA/pilA2) [Thermus thermophilus strain SNM3-3]
MRKGLTLVEVLVTLVIMGIAFAALLTSQLANLRASAQARFATDAKAAAVQVLERRSAEVLKSEIVPALSPYKDAPLDPDN
PSGNWRSFYFVDYYFSCPTRVAPSPKQRGGSVANLRPGLTCSGTETIFGIPVAWDIRGENGILGEGVVTVVVTATHPRGP
KVTLGRRVTCYDVYPSPTQDQPAPCPPPGGGRP

Nucleotide


Download         Length: 582 bp        

>NTDB_id=93013 MWM13_RS06830 WP_011173284.1 1279513..1280094(+) (pilA/pilA2) [Thermus thermophilus strain SNM3-3]
ATGAGAAAAGGGCTCACTCTCGTGGAAGTCCTTGTCACCTTGGTCATCATGGGTATCGCCTTTGCCGCCTTGTTGACGAG
CCAGCTTGCCAACCTCCGGGCGAGCGCCCAGGCCCGCTTCGCCACCGACGCCAAGGCCGCCGCCGTCCAGGTCCTAGAGC
GCCGCTCGGCCGAGGTGCTGAAAAGCGAAATCGTCCCGGCCTTAAGCCCCTACAAGGACGCCCCCTTGGACCCGGACAAC
CCTTCGGGAAACTGGCGGAGCTTTTACTTCGTGGACTACTACTTCTCCTGCCCCACCCGGGTGGCCCCTTCCCCTAAGCA
GAGGGGAGGGTCTGTAGCCAACCTTCGTCCCGGCCTCACCTGCTCGGGCACGGAGACGATCTTTGGCATCCCCGTGGCCT
GGGACATCCGCGGGGAAAACGGCATCCTGGGCGAGGGCGTGGTGACGGTGGTGGTCACGGCCACCCATCCCCGGGGGCCC
AAGGTCACCCTGGGCCGCCGGGTCACCTGCTACGACGTCTACCCCTCCCCCACCCAGGACCAGCCCGCCCCTTGCCCACC
TCCTGGAGGTGGTCGCCCATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q72JC3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA2 Thermus thermophilus HB27

100

100

1

Multiple sequence alignment