Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   MWM12_RS07300 Genome accession   NZ_AP025603
Coordinates   1341561..1342184 (+) Length   207 a.a.
NCBI ID   WP_011173433.1    Uniprot ID   Q72IW7
Organism   Thermus thermophilus strain SNM1-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1336561..1347184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM12_RS07275 (TthSNM17_14240) - 1336705..1337805 (-) 1101 WP_244340636.1 transposase -
  MWM12_RS07280 (TthSNM17_14250) - 1338345..1338695 (+) 351 Protein_1411 HD domain-containing phosphohydrolase -
  MWM12_RS07285 (TthSNM17_14260) - 1338746..1339237 (+) 492 WP_011173430.1 DUF5317 domain-containing protein -
  MWM12_RS07290 (TthSNM17_14270) - 1339292..1340296 (+) 1005 WP_244340638.1 homoisocitrate dehydrogenase -
  MWM12_RS07295 (TthSNM17_14280) pilM 1340435..1341568 (+) 1134 WP_244340640.1 type IV pilus assembly protein PilM Machinery gene
  MWM12_RS07300 (TthSNM17_14290) pilN 1341561..1342184 (+) 624 WP_011173433.1 competence protein Machinery gene
  MWM12_RS07305 (TthSNM17_14300) pilO 1342174..1342755 (+) 582 WP_011173434.1 type 4a pilus biogenesis protein PilO Machinery gene
  MWM12_RS07310 (TthSNM17_14310) pilW 1342752..1343630 (+) 879 WP_244340642.1 competence protein Machinery gene
  MWM12_RS07315 (TthSNM17_14320) pilQ 1343627..1345900 (+) 2274 WP_244340644.1 secretin N-terminal domain-containing protein Machinery gene
  MWM12_RS07320 (TthSNM17_14330) aroC 1345959..1347110 (+) 1152 WP_143586076.1 chorismate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 23035.51 Da        Isoelectric Point: 9.7530

>NTDB_id=92989 MWM12_RS07300 WP_011173433.1 1341561..1342184(+) (pilN) [Thermus thermophilus strain SNM1-7]
MIRLNLLPKNLRRRVEPGWWRLLALLFALVVLGVLGLVHYTAYTELSLAKAERDQLQAEVEALRPFIAELGRLQEERKAL
EALLAIREGLEKNAVPWSQYLAAFINQIPRAGGRLEVALRSVSARALSEEEAARLAQEGTYDGKRIRVEFALQGEALSRE
ALVRFIRAFETSPRFGIEFQGASLDEGRGLYTFSARVGATGGESGAR

Nucleotide


Download         Length: 624 bp        

>NTDB_id=92989 MWM12_RS07300 WP_011173433.1 1341561..1342184(+) (pilN) [Thermus thermophilus strain SNM1-7]
TTGATTAGGCTGAACCTTCTCCCCAAAAACCTCCGGCGCCGGGTGGAGCCGGGCTGGTGGCGCCTCCTCGCCCTCCTCTT
CGCCCTCGTGGTCCTCGGGGTCTTGGGGCTCGTCCACTACACCGCCTACACCGAGCTCTCCCTGGCCAAGGCGGAGCGGG
ACCAGCTCCAGGCGGAGGTGGAGGCCTTGAGGCCCTTCATCGCCGAGCTCGGCCGCCTGCAGGAGGAGCGCAAGGCCCTC
GAGGCCCTCCTCGCCATCCGGGAAGGCCTGGAGAAAAACGCCGTGCCCTGGTCCCAGTACCTGGCCGCCTTCATCAACCA
GATCCCCAGGGCGGGGGGGCGCCTCGAGGTGGCCCTTCGCTCGGTGAGCGCCCGCGCCCTCTCGGAAGAGGAGGCCGCCC
GCCTGGCCCAGGAAGGCACCTACGACGGCAAGCGGATCCGGGTGGAGTTCGCCCTCCAGGGGGAGGCCCTGAGCCGGGAG
GCCCTGGTCCGGTTCATCCGGGCCTTTGAGACCTCCCCCCGGTTCGGCATTGAGTTCCAGGGGGCCTCCCTGGACGAGGG
CCGGGGGCTTTACACCTTCAGCGCCCGCGTGGGCGCGACGGGGGGTGAAAGCGGTGCTCGCTAG

Domains

Predicted by InterproScan.

PilN biogenesis protein dimerization domain (PilN_bio_d)

(97-200)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 2YCH

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Thermus thermophilus HB27

100

100

1

Multiple sequence alignment