Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   V1502_RS05590 Genome accession   NZ_CP143546
Coordinates   1133585..1137361 (+) Length   1258 a.a.
NCBI ID   WP_409254286.1    Uniprot ID   -
Organism   Bacillus sp. SCS-153A     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1128585..1142361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1502_RS05585 (V1502_05585) addB 1130107..1133595 (+) 3489 WP_409253386.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  V1502_RS05590 (V1502_05590) addA 1133585..1137361 (+) 3777 WP_409254286.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  V1502_RS05595 (V1502_05595) - 1137802..1138110 (+) 309 WP_409253387.1 HNH endonuclease -
  V1502_RS05600 (V1502_05600) - 1138129..1138347 (-) 219 WP_006837409.1 spore germination protein -
  V1502_RS05605 (V1502_05605) - 1138413..1138787 (-) 375 WP_409253388.1 spore germination protein GerPE -
  V1502_RS05610 (V1502_05610) - 1138794..1138973 (-) 180 WP_409253389.1 spore gernimation protein GerPD -
  V1502_RS05615 (V1502_05615) gerPC 1138948..1139586 (-) 639 WP_409253390.1 spore germination protein GerPC -
  V1502_RS05620 (V1502_05620) - 1139610..1139834 (-) 225 WP_409253391.1 spore germination protein GerPB -
  V1502_RS05625 (V1502_05625) - 1139845..1140066 (-) 222 WP_409254287.1 spore germination protein -
  V1502_RS05630 (V1502_05630) - 1140198..1141376 (+) 1179 WP_409253392.1 DUF418 domain-containing protein -
  V1502_RS05635 (V1502_05635) - 1141413..1142318 (+) 906 WP_409253393.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 1258 a.a.        Molecular weight: 144263.07 Da        Isoelectric Point: 4.7554

>NTDB_id=929546 V1502_RS05590 WP_409254286.1 1133585..1137361(+) (addA) [Bacillus sp. SCS-153A]
MNHNLPVKPEGATWTDDQWKAIWAKGRDILVAAAAGSGKTAVLVERIIQKILDKEDGINVDELLVVTFTNASAAEMRHRI
GEALEKAISADPGSYHLRKQLSLLNRASISTLHSFCLEVIRKYYYLIDIDPGFRILDDTEGELLRDEVLDELFEEEYGKE
GNDSFYKLVDTFTNDRSDAALQDMIRKLYDFSRSHPNPDAWLGDLVKMYDVEDGDRIEDLPFMEALKFDIILSLTGAQSL
FQRAYDLTKEPGGPAPRAENFLDDLGIAEQLLSSFQNSWSEGQALFQSIKFSRLKPCKGDDYNPELVEEAKALRDKGKKM
IEKLAEDFFSRKPETYLKDMKKMKGVVASLVEAVRGFSQKFNELKNEKGVVDFADLEHYCLEIMTDTSDEEELKPSFAAL
EYRKQFKEVLVDEYQDTNLVQESILGLVTEDEESRGNLFMVGDVKQSIYRFRLAEPNLFLGKYNRFTPAGTETGMKIDLS
RNFRSRLEVLEGTNFLFKQIMGVNVGDIEYNHEALLVKGAAYPEDEQYPIEVAVINQSDDEVPQADSNGDMEGLVYDEAD
LEKSVIEARYMAQKVKELISEGKQVYDAKAGLHRKLQYKDIVILLRSMPWAPEIMEEFKKNGIPIYANLSSGYFEATEVA
IMLSLLKVIDNPYQDIPLVSVLRSPIVGIDEEGLAEVRIHSKSGTYYEAVQRFISKKPSEVNNEEVHEKLGEFLSNLTVW
RTKARQGSLADLVWQLYRDTRFYDFAGGMPGGKQRQANLRALYDRARQYEETSFRGLFRFLRFIDRMRERGDDLGAARAL
SEQEDVVRMMTIHSSKGLEFPVVFVAGLSRQFNLMDLNQSYLLDKEFGFASKYLDPEKRISYPSLPQLAFKRKKRLETIA
EEMRVLYVALTRAKEKLYLIGTVKDADKSIKQWTEAARQTEWLLPDYERAKALSFMDWIGPALVRHRDCAPLTEGQSPAH
SLPSEIHDHPSCWKLEVIPKSQFMIMEGEDEEEEIDWQDKVKAGLPVDLESSDKAEVVERLSWKYSYPEAAVLPSKQSVS
EIKRMFEISDEASSDQMVRKIQKPVFRRPKFLQSKQLTPAEIGTAMHMVMQHISFEEMPDDNSLQTLLADLKRKELLTEE
QAEAIDQKKILAFFQGETGQRVLNAREIHREVPFSMAVPVQEINPTVSAKDSETVLVQGIIDLIFRDDQGLVLLDYKTDS
IDERYKGGYEEARPILLERYRVQVGLYTKAIERIWKEAVNERYLYFFDVARELKVEKE

Nucleotide


Download         Length: 3777 bp        

>NTDB_id=929546 V1502_RS05590 WP_409254286.1 1133585..1137361(+) (addA) [Bacillus sp. SCS-153A]
ATGAATCATAATCTTCCTGTAAAACCCGAAGGTGCTACTTGGACCGATGACCAATGGAAAGCAATCTGGGCAAAAGGCCG
GGATATCCTGGTGGCCGCGGCAGCCGGTTCCGGTAAGACGGCTGTCCTTGTTGAGAGGATCATTCAAAAAATTCTCGACA
AAGAAGACGGCATTAATGTGGACGAGCTGCTTGTCGTGACGTTTACAAATGCCTCAGCAGCCGAGATGAGACATCGAATC
GGAGAGGCGTTGGAAAAAGCGATTTCGGCCGACCCCGGTTCCTACCACCTCAGAAAGCAGCTGAGCTTGCTAAACAGGGC
ATCCATCTCAACTCTCCATTCCTTTTGTCTGGAAGTCATCCGTAAATATTATTACTTGATTGACATTGACCCAGGGTTCC
GGATCTTGGATGACACGGAAGGTGAACTGCTGAGGGATGAAGTCCTTGACGAATTATTTGAGGAGGAATATGGAAAAGAG
GGAAATGACTCTTTTTATAAACTGGTTGATACGTTCACGAACGACCGCAGTGATGCTGCCCTTCAGGATATGATACGCAA
ACTGTACGACTTTTCCAGATCCCACCCGAACCCTGATGCATGGCTAGGGGATCTGGTGAAAATGTATGATGTCGAAGATG
GGGACCGAATTGAAGACCTGCCGTTTATGGAAGCGTTGAAGTTTGATATTATTCTAAGTTTGACAGGTGCTCAGTCCTTA
TTTCAGCGTGCATATGACCTGACGAAAGAGCCAGGAGGACCGGCGCCAAGAGCAGAAAACTTTCTGGATGACCTGGGGAT
AGCTGAACAGCTCCTCTCTTCTTTTCAGAATTCATGGAGTGAAGGGCAGGCGTTGTTCCAAAGCATCAAGTTTTCAAGGC
TGAAGCCATGCAAGGGCGATGATTATAATCCTGAACTGGTCGAGGAAGCCAAGGCCCTCAGGGATAAAGGCAAGAAAATG
ATCGAGAAACTGGCTGAAGACTTCTTTTCCCGAAAACCAGAAACCTACTTGAAAGATATGAAGAAAATGAAAGGGGTAGT
GGCCTCTCTGGTGGAAGCCGTGAGAGGTTTCAGCCAGAAATTCAATGAGTTGAAAAATGAAAAAGGTGTCGTGGATTTTG
CGGACCTTGAACATTACTGTCTGGAAATTATGACGGACACCTCAGACGAGGAAGAGTTAAAACCCTCGTTTGCGGCTTTG
GAATATAGAAAGCAGTTCAAAGAAGTGCTGGTTGACGAATACCAGGATACCAACCTGGTGCAAGAATCCATCCTCGGGCT
CGTCACAGAGGATGAAGAGTCCAGGGGAAATCTTTTCATGGTAGGGGATGTCAAGCAGTCGATTTACCGATTCCGATTGG
CAGAACCGAACTTATTCCTTGGCAAGTATAACCGCTTCACCCCAGCCGGAACAGAAACAGGAATGAAGATTGATTTATCC
AGGAATTTTCGGAGCCGCTTGGAAGTCCTGGAAGGAACCAATTTTTTGTTCAAACAAATCATGGGAGTGAATGTCGGGGA
TATTGAATACAATCATGAGGCACTGCTGGTTAAAGGTGCAGCCTATCCGGAAGATGAACAATACCCGATAGAGGTGGCTG
TCATAAATCAGTCGGATGACGAAGTGCCGCAAGCAGATTCGAATGGGGATATGGAAGGTCTCGTTTATGATGAAGCTGAT
CTGGAAAAGTCCGTCATTGAAGCGAGATATATGGCCCAAAAGGTCAAAGAACTCATCAGTGAAGGGAAACAAGTGTATGA
TGCGAAGGCAGGACTCCACCGAAAGCTTCAATACAAGGATATTGTCATTCTTTTACGTTCCATGCCTTGGGCTCCTGAGA
TCATGGAAGAATTCAAGAAAAACGGAATCCCGATTTACGCTAATTTATCATCAGGCTATTTTGAAGCAACTGAAGTGGCC
ATCATGCTTTCTTTATTAAAAGTAATCGATAACCCTTATCAGGATATCCCGCTTGTATCTGTACTGCGCTCACCGATTGT
CGGCATTGACGAAGAAGGGCTTGCTGAAGTGCGAATTCATTCAAAGTCCGGTACATATTATGAAGCCGTCCAAAGGTTTA
TCAGCAAGAAACCAAGTGAAGTCAACAATGAAGAAGTTCATGAGAAATTAGGGGAGTTTCTATCAAACTTGACGGTCTGG
AGGACAAAAGCACGCCAAGGGTCGCTTGCTGATTTGGTTTGGCAGCTGTACCGCGATACAAGGTTTTATGACTTTGCAGG
CGGAATGCCGGGTGGAAAGCAGAGGCAGGCCAACCTTAGGGCATTGTATGACCGGGCGCGTCAGTATGAGGAGACTTCTT
TCCGCGGGTTGTTCCGTTTTCTGCGCTTCATAGACAGGATGCGCGAACGCGGTGATGATCTTGGAGCAGCAAGGGCTTTA
AGTGAACAGGAAGATGTGGTAAGGATGATGACCATCCACTCCAGTAAAGGTTTGGAGTTTCCGGTCGTATTCGTCGCAGG
TTTATCCAGGCAGTTCAACCTGATGGATTTGAACCAATCGTATTTACTGGATAAGGAGTTCGGTTTTGCCTCGAAATACC
TGGATCCTGAAAAAAGAATCAGCTATCCGTCGCTGCCTCAGCTTGCGTTTAAGAGGAAAAAAAGGCTTGAAACGATTGCC
GAAGAGATGAGGGTATTGTACGTTGCCTTGACGAGAGCAAAAGAAAAGCTGTATCTCATTGGGACAGTGAAGGATGCGGA
TAAGTCGATTAAGCAGTGGACGGAAGCTGCAAGGCAGACGGAATGGCTTCTTCCGGATTATGAAAGGGCCAAAGCGTTGA
GTTTCATGGACTGGATCGGCCCGGCACTTGTAAGGCACCGTGACTGCGCACCATTGACTGAAGGGCAGTCTCCCGCACAT
TCACTTCCTTCTGAAATCCATGATCATCCTTCTTGCTGGAAACTCGAAGTGATCCCTAAATCACAGTTTATGATCATGGA
AGGAGAAGACGAGGAAGAGGAGATCGATTGGCAGGATAAGGTGAAAGCAGGACTTCCGGTTGATCTGGAATCTTCCGATA
AGGCAGAAGTTGTTGAACGATTGTCATGGAAATATTCCTATCCGGAAGCGGCAGTGCTGCCTTCGAAACAATCCGTCTCG
GAAATCAAGAGGATGTTTGAAATCTCTGATGAAGCAAGCAGTGACCAGATGGTGAGAAAAATTCAAAAACCGGTCTTTAG
ACGACCTAAGTTCCTGCAATCTAAACAACTGACTCCTGCAGAAATCGGCACGGCGATGCACATGGTCATGCAGCACATTT
CCTTTGAGGAAATGCCTGATGATAACTCGCTGCAGACACTGCTGGCTGATCTGAAGCGAAAAGAACTGTTGACTGAAGAA
CAGGCAGAGGCCATCGATCAGAAGAAAATTCTGGCTTTCTTCCAAGGCGAAACGGGACAAAGGGTTCTGAACGCCCGGGA
AATTCACCGCGAGGTCCCATTCAGTATGGCGGTTCCGGTTCAGGAAATTAATCCAACTGTTTCTGCCAAGGACTCAGAAA
CAGTACTGGTCCAGGGGATCATCGACCTGATCTTCCGGGATGACCAGGGGCTCGTGCTTCTGGATTACAAAACAGACAGC
ATTGATGAAAGGTACAAAGGCGGATACGAAGAAGCAAGGCCGATCCTGCTGGAACGCTATCGTGTGCAGGTCGGTTTATA
TACAAAAGCCATCGAGAGGATTTGGAAGGAAGCTGTCAATGAACGCTACCTGTACTTTTTCGATGTCGCCCGTGAACTGA
AAGTGGAAAAAGAATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-466)

UvrD-like helicase C-terminal domain (UvrD_C)

(477-902)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1070-1239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

55.012

99.126

0.545