Detailed information NTG0929545

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: V1502_RS05585
• Genome accession: NZ_CP143546
• Coordinates: 1130107..1133595 (+)
• Length: 1162 a.a.
• NCBI ID: WP_409253386.1
• Uniprot ID: -
• Organism: Bacillus sp. SCS-153A
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1125107..1138595

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V1502_RS05575 (V1502_05575)	fabG	1126946..1127686 (+)	741	WP_409253384.1	3-oxoacyl-ACP reductase FabG	-
V1502_RS05580 (V1502_05580)	-	1127706..1128881 (+)	1176	WP_409253385.1	thiolase family protein	-
V1502_RS05585 (V1502_05585)	addB	1130107..1133595 (+)	3489	WP_409253386.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
V1502_RS05590 (V1502_05590)	addA	1133585..1137361 (+)	3777	WP_409254286.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
V1502_RS05595 (V1502_05595)	-	1137802..1138110 (+)	309	WP_409253387.1	HNH endonuclease	-
V1502_RS05600 (V1502_05600)	-	1138129..1138347 (-)	219	WP_006837409.1	spore germination protein	-

Sequence

Protein

• Length: 1162 a.a.
• Molecular weight: 133438.32 Da
• Isoelectric Point: 5.6593

>NTDB_id=929545 V1502_RS05585 WP_409253386.1 1130107..1133595(+) (addB) [Bacillus sp. SCS-153A]
MSVRFILGRAGTGKTNMILNEIKEKLRQEPNGSPVIYLVPDQMTFLSEYNLVNEEGINGMIRAQVYSFTRLAWRILQETG
GLNRYHLSNIGLNMLIRKIIDDKKDQLKLFSKASDKAGFIEHVEVMLTEFKRYCISPEELSEKQEGWGERGQESKALADK
LHDLQLIYESFEENLSGKYIDSEDYFTLLEESIGKADFLKDAEIFIDGFHSFTPQEYAVVGKLMDHCPRVTIALTAEPDF
GSEQNDLFRMTSETYENLLQLAMASRGKVEDKVVLGETSRFQNKGLQHIEANFEKRPVLPFEDESGMNIIEASNRRAEIE
GVAREIRRLARTENYRYKDMAVLVRNGQEYQDIIETVFYDYDIPYFIDQKRKMLNHPLIELIRSTLEIMNSNWRYEPVFR
AVKTDLLFPYDRNPQQLREKMDKLENYVLAYGIQGDKWTKKDRWIYRRFRGLEQADMPQTDKEREMEREINELRLFISAP
ILRFGRRLKKGKTGQDLCEILYLYLEELDIPAKLERLRLEAENRGDIISAREHDQAWNAVMDLLDQFVEILGDEEVTMKK
FASIIDAGIESMRFSLVPPAVDQVMIANLEQSRLSDLKAAFVIGVNDGVLPAKITEDGIFSDEDREKLGASGLTIAPGSK
TRLLDEEFIAYKAFTTPSERLYVSYPIANEEGKALLPSPYIKRLSELCPQSEISLLVNDPSELPKNEQLSYVCHPNVTIS
YLTSGLQAKKRGYHLEDFWWDAYNYYVSDSVWKGRARHILSSLFYQNKGKKLTEATSQELYGEEILASVSRMELFHGCPF
SHFANHGLKLRERDIYRLEAPDIGDMFHAALKWISEEIDKHGLSWKQLTKTQCLELAREAVLSLAPKLQHQILLSSNRHH
YIKKKLQDVIGRASLILSEHAKASGFAPVGLELGFGPKAELPPFAFTLKNGTKMALQGRIDRVDKAESDEGVYLRIIDYK
SSARDLDLTEVYYGLSLQMLTYLDIVLTHSRKLIGAEADPAGVFYFHVHNPMVNSKKILTLEEIEEEIFKSFKMKGLVLG
ETDVVRMMDQSLESGSSNIISAGLKKDGTLSSKTKSADRTQFDVMRSHVRNLYKKSGNDIMSGRTDIAPYKLKDRTPCQY
CAFKPVCQFDTSLEENEYRLFTEDKPADLMEKMKGERDAHES

Nucleotide

• Length: 3489 bp

>NTDB_id=929545 V1502_RS05585 WP_409253386.1 1130107..1133595(+) (addB) [Bacillus sp. SCS-153A]
ATGTCGGTCAGATTTATTTTGGGACGAGCCGGAACAGGCAAAACCAATATGATTTTGAATGAAATCAAAGAAAAGCTGAG
ACAGGAGCCAAATGGCAGTCCTGTCATCTATCTTGTTCCAGACCAGATGACATTTCTTTCTGAATACAATTTGGTGAACG
AAGAAGGGATAAACGGGATGATCCGGGCGCAGGTTTACAGTTTTACAAGGCTTGCATGGAGAATTCTGCAGGAAACAGGA
GGTCTTAATCGCTATCACTTATCAAACATCGGTCTTAACATGCTGATCCGTAAAATAATCGATGATAAAAAAGATCAGTT
GAAATTATTTTCAAAGGCCTCGGATAAAGCAGGCTTTATTGAGCATGTAGAAGTGATGCTTACCGAGTTTAAGCGATACT
GTATCTCACCGGAAGAACTGTCAGAGAAACAGGAAGGCTGGGGAGAGCGCGGGCAGGAATCCAAGGCATTGGCAGACAAG
CTTCATGACCTGCAGCTCATCTATGAAAGCTTTGAAGAAAACCTGTCAGGAAAGTACATCGATTCAGAAGATTATTTTAC
TCTATTGGAAGAATCAATCGGGAAGGCCGATTTCCTAAAAGATGCTGAAATTTTTATAGATGGCTTCCACAGTTTTACAC
CGCAGGAATATGCGGTGGTTGGAAAACTGATGGATCATTGTCCAAGGGTGACGATTGCCCTTACGGCAGAGCCTGACTTC
GGGAGCGAACAAAACGATTTGTTCAGGATGACTTCTGAAACCTATGAGAACCTGCTGCAGCTTGCGATGGCCTCCAGAGG
AAAGGTTGAAGACAAGGTTGTACTCGGGGAAACGAGCCGCTTCCAGAACAAAGGGCTTCAACATATCGAGGCGAATTTTG
AAAAGCGGCCTGTATTGCCGTTTGAGGATGAATCCGGAATGAACATTATAGAGGCCTCCAACCGCAGGGCTGAAATTGAA
GGGGTGGCAAGGGAGATTCGGCGCCTTGCCCGAACAGAGAATTATCGTTATAAGGATATGGCTGTCCTTGTGCGAAATGG
TCAGGAGTATCAAGATATCATTGAAACGGTATTTTATGATTATGACATTCCCTACTTTATTGATCAGAAACGAAAAATGC
TTAATCATCCGCTGATTGAACTGATTCGTTCAACACTTGAAATCATGAATAGCAATTGGCGCTATGAACCTGTTTTCCGG
GCAGTCAAAACGGATCTTCTGTTTCCGTATGACCGTAATCCCCAGCAGCTGAGGGAAAAGATGGACAAGCTCGAAAATTA
TGTCCTTGCTTATGGAATACAGGGAGATAAATGGACAAAAAAGGATCGCTGGATTTATCGGAGATTCCGCGGCCTGGAAC
AGGCTGATATGCCGCAGACAGACAAAGAAAGAGAAATGGAGCGGGAGATCAATGAGCTTCGCTTGTTTATCTCTGCACCG
ATCCTGCGATTCGGCAGGCGTTTGAAAAAGGGGAAGACCGGACAAGACCTGTGTGAAATTCTTTACCTTTATCTAGAGGA
ACTAGATATCCCTGCAAAACTCGAACGGCTTCGTCTTGAAGCGGAGAACCGCGGTGATATCATCTCTGCAAGAGAACATG
ATCAGGCCTGGAATGCCGTGATGGATCTGCTGGACCAATTTGTCGAGATCTTGGGTGATGAGGAAGTCACGATGAAAAAG
TTTGCTTCCATCATTGATGCCGGAATTGAATCAATGAGGTTCTCTTTGGTCCCTCCTGCTGTGGATCAGGTCATGATTGC
CAATCTTGAACAATCAAGGCTCTCAGACCTCAAGGCGGCTTTTGTAATTGGTGTAAATGATGGCGTCCTTCCGGCAAAAA
TCACTGAGGATGGAATTTTTTCAGATGAGGACAGGGAAAAGCTTGGGGCATCAGGATTAACTATTGCACCCGGCAGCAAG
ACCCGCCTGCTTGATGAAGAGTTCATTGCCTATAAGGCATTCACGACACCATCCGAAAGGCTTTATGTTTCTTATCCAAT
TGCGAATGAAGAGGGAAAAGCCCTTCTGCCGTCTCCCTATATTAAGAGACTGTCTGAACTGTGCCCGCAGTCGGAAATCA
GTCTTCTGGTCAATGATCCGTCTGAATTGCCGAAAAATGAACAGTTATCCTATGTCTGTCATCCAAATGTAACGATTTCC
TATCTGACTTCTGGGCTTCAGGCGAAAAAGCGAGGCTATCATCTGGAAGATTTTTGGTGGGATGCGTATAACTATTATGT
CTCGGATTCGGTGTGGAAAGGACGCGCCAGACATATACTTTCAAGCTTGTTTTATCAAAATAAAGGTAAAAAACTTACAG
AAGCAACAAGCCAGGAGTTGTATGGGGAAGAAATATTGGCAAGTGTCTCAAGGATGGAACTGTTCCACGGGTGTCCGTTC
TCTCATTTTGCCAACCATGGATTAAAACTTCGCGAACGGGATATTTATCGCCTGGAAGCACCGGATATCGGGGATATGTT
CCATGCAGCCCTTAAATGGATCTCCGAGGAAATTGATAAACATGGACTATCATGGAAACAGCTGACCAAAACACAGTGCC
TAGAATTGGCCAGGGAAGCCGTTTTATCATTGGCGCCCAAGCTTCAGCACCAGATTTTGCTCAGCTCGAACCGCCATCAT
TATATAAAGAAGAAACTGCAGGATGTCATCGGCAGAGCTTCCCTGATTTTGAGCGAACATGCCAAAGCAAGCGGCTTTGC
TCCTGTGGGCCTTGAATTGGGGTTTGGCCCGAAAGCGGAACTGCCGCCGTTTGCCTTTACATTGAAAAATGGCACAAAGA
TGGCCCTCCAAGGCAGGATTGACAGGGTGGATAAAGCGGAGAGCGATGAAGGTGTCTATCTGCGAATCATCGACTATAAG
TCAAGTGCGAGAGACCTTGATCTTACTGAAGTGTACTATGGGTTGTCTCTGCAAATGCTGACATACCTGGATATCGTTTT
GACGCATTCACGGAAGTTGATTGGGGCAGAAGCCGATCCGGCAGGGGTATTTTACTTCCATGTCCATAACCCGATGGTCA
ATAGCAAAAAGATTCTCACACTCGAAGAAATCGAAGAGGAGATTTTCAAAAGCTTTAAAATGAAGGGACTGGTATTGGGA
GAAACCGATGTCGTCAGGATGATGGACCAGTCATTAGAGTCAGGAAGTTCCAATATCATTTCTGCAGGATTGAAAAAAGA
CGGAACGTTGTCATCTAAGACCAAATCGGCAGACCGGACCCAGTTTGATGTGATGCGCTCGCATGTAAGGAACCTGTACA
AGAAATCCGGGAATGACATTATGTCCGGAAGAACGGATATCGCTCCATATAAATTAAAAGACAGAACGCCTTGTCAATAT
TGTGCGTTCAAACCGGTTTGCCAGTTTGATACTTCATTGGAAGAAAACGAATATCGTCTTTTTACAGAGGACAAACCGGC
AGATTTAATGGAAAAGATGAAAGGAGAACGAGATGCCCATGAATCATAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (284-665)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (787-1128)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	51.973	100	0.522