Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   V0I11_RS06515 Genome accession   NZ_CP143490
Coordinates   1397008..1397553 (-) Length   181 a.a.
NCBI ID   WP_330453285.1    Uniprot ID   -
Organism   Pasteurella multocida strain FCf147     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1392008..1402553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V0I11_RS06490 (V0I11_06490) - 1392222..1392659 (-) 438 WP_010907449.1 methyltransferase family protein -
  V0I11_RS06495 (V0I11_06495) - 1392868..1393479 (-) 612 WP_330453282.1 YfgM family protein -
  V0I11_RS06500 (V0I11_06500) hisS 1393498..1394769 (-) 1272 WP_010907448.1 histidine--tRNA ligase -
  V0I11_RS06505 (V0I11_06505) ispG 1394799..1395902 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  V0I11_RS06510 (V0I11_06510) - 1395929..1396885 (-) 957 WP_169323501.1 helix-turn-helix domain-containing protein -
  V0I11_RS06515 (V0I11_06515) pilF/pilF2 1397008..1397553 (-) 546 WP_330453285.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  V0I11_RS06520 (V0I11_06520) - 1397593..1398777 (-) 1185 WP_061406244.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  V0I11_RS06525 (V0I11_06525) - 1398958..1400211 (-) 1254 WP_330453289.1 AI-2E family transporter -
  V0I11_RS06530 (V0I11_06530) glnB 1400212..1400550 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  V0I11_RS06535 (V0I11_06535) mog 1400595..1401173 (-) 579 WP_330453291.1 molybdopterin adenylyltransferase -
  V0I11_RS06540 (V0I11_06540) - 1401199..1402128 (-) 930 WP_005725108.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20873.38 Da        Isoelectric Point: 8.2666

>NTDB_id=929176 V0I11_RS06515 WP_330453285.1 1397008..1397553(-) (pilF/pilF2) [Pasteurella multocida strain FCf147]
MNRTFRFGIASIFSLFLSACVSQEGNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=929176 V0I11_RS06515 WP_330453285.1 1397008..1397553(-) (pilF/pilF2) [Pasteurella multocida strain FCf147]
ATGAACCGCACTTTTCGATTTGGGATCGCAAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
AACGTTTGATTCACATGAGGCAGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

45.405

100

0.464