Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   V1209_RS06765 Genome accession   NZ_CP143255
Coordinates   1509303..1509851 (-) Length   182 a.a.
NCBI ID   WP_011848557.1    Uniprot ID   A3N1S3
Organism   Actinobacillus pleuropneumoniae strain ZJNH2023     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1504303..1514851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1209_RS06750 (V1209_06730) - 1504785..1505306 (+) 522 WP_193327846.1 SprT family zinc-dependent metalloprotease -
  V1209_RS06755 (V1209_06735) - 1505411..1506697 (+) 1287 WP_005615681.1 NAD(P)/FAD-dependent oxidoreductase -
  V1209_RS06760 (V1209_06740) - 1506889..1509228 (-) 2340 WP_011848556.1 Tex family protein -
  V1209_RS06765 (V1209_06745) pilF 1509303..1509851 (-) 549 WP_011848557.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  V1209_RS06770 (V1209_06750) - 1509905..1511086 (-) 1182 WP_009875290.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  V1209_RS06795 (V1209_06775) gltX 1511990..1513429 (+) 1440 WP_354731912.1 glutamate--tRNA ligase -
  V1209_RS06805 (V1209_06785) yefM 1513767..1514018 (+) 252 WP_005605062.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  V1209_RS06810 (V1209_06790) - 1514015..1514275 (+) 261 WP_009875288.1 Txe/YoeB family addiction module toxin -
  V1209_RS06815 (V1209_06795) - 1514432..1514599 (-) 168 WP_009875287.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20671.35 Da        Isoelectric Point: 6.9479

>NTDB_id=928287 V1209_RS06765 WP_011848557.1 1509303..1509851(-) (pilF) [Actinobacillus pleuropneumoniae strain ZJNH2023]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=928287 V1209_RS06765 WP_011848557.1 1509303..1509851(-) (pilF) [Actinobacillus pleuropneumoniae strain ZJNH2023]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCCGTGTTATTCGGTTGTTCTTCTCAGCTTCC
AACCTCCCAAACTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGTATTAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTATTTGCCTCACTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCATGCGGAACAGGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3N1S3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

67.021

100

0.692
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44