Detailed information NTG0092619

Bioinformatically predicted

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: QUE39_RS07040
• Genome accession: NZ_AP025540
• Coordinates: 1321310..1323583 (+)
• Length: 757 a.a.
• NCBI ID: WP_011228639.1
• Uniprot ID: Q5SII6
• Organism: Thermus thermophilus strain HB8_001
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1316310..1328583

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QUE39_RS07010 (TthHB8_13610)	-	1316432..1316923 (+)	492	WP_011173430.1	DUF5317 domain-containing protein	-
QUE39_RS07015 (TthHB8_13620)	-	1316978..1317982 (+)	1005	WP_011173431.1	homoisocitrate dehydrogenase	-
QUE39_RS07020 (TthHB8_13630)	pilM	1318118..1319251 (+)	1134	WP_011228636.1	type IV pilus assembly protein PilM	Machinery gene
QUE39_RS07025 (TthHB8_13640)	pilN	1319244..1319867 (+)	624	WP_011228637.1	competence protein	Machinery gene
QUE39_RS07030 (TthHB8_13650)	pilO	1319857..1320438 (+)	582	WP_011173434.1	type 4a pilus biogenesis protein PilO	Machinery gene
QUE39_RS07035 (TthHB8_13660)	pilW	1320435..1321313 (+)	879	WP_011228638.1	competence protein	Machinery gene
QUE39_RS07040 (TthHB8_13670)	pilQ	1321310..1323583 (+)	2274	WP_011228639.1	secretin N-terminal domain-containing protein	Machinery gene
QUE39_RS07045 (TthHB8_13680)	aroC	1323642..1324793 (+)	1152	WP_011228640.1	chorismate synthase	-
QUE39_RS07050 (TthHB8_13690)	-	1324818..1325372 (+)	555	WP_008632876.1	shikimate kinase	-
QUE39_RS07055 (TthHB8_13700)	-	1325350..1326396 (+)	1047	WP_011228641.1	3-dehydroquinate synthase family protein	-
QUE39_RS07060 (TthHB8_13710)	rsmF	1326442..1327812 (-)	1371	WP_011228642.1	16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF	-
QUE39_RS07065 (TthHB8_13720)	-	1327865..1328242 (+)	378	WP_011173441.1	4'-phosphopantetheinyl transferase superfamily protein	-
QUE39_RS07070 (TthHB8_13730)	-	1328220..1328573 (-)	354	WP_011228643.1	DUF4870 domain-containing protein	-

Sequence

Protein

• Length: 757 a.a.
• Molecular weight: 82690.81 Da
• Isoelectric Point: 5.2152

>NTDB_id=92619 QUE39_RS07040 WP_011228639.1 1321310..1323583(+) (pilQ) [Thermus thermophilus strain HB8_001]
MKSAWIRAAVIALAGLGAFALAGSFPEEPRFQAPVNLKVSESQVKAGQTLPLDVVLEALARSVGLQPLIYRAYDPSGDPA
KAQPPLPNIKLDFQGKPFREVWDLLFATYGNQYSLDYLFLPPDVVVVAPTQVITALVDAPSRTGAMERRPYIVGIPEIAY
KRTETDAQGQPRTVVNIEGAKAWVQNDLLPFLSREAAGLNVNWIVVEEGGRLKAVLSVLATPEQHARFSDILQRAGIDFR
PLPALAQPKPRVEKTYTLTYATFPELLAFLQSRLPEAQISVVPTNPQRAIVLATEEDHARLSELLKTADVPKTVRRVYAL
QNLTFAEAQERLKPLLEKDLKGARLESLPGNPKALLLEAPEAEHALFAEILKALDVPPQAPQAPQEATLRRLYPLRYANA
EQVAPFLAREVPGIVVQTVPGQPVLSVRGTEKQLAEVESLLAQIDRAPEQGPPVFQRAYQLSNAKAVELAQVLQEALKAR
QAQNQGQQNQALPTREATVVADPRTNTLIVTGTQEDLALVEGLIPKLDQPVPQVSLRVRIQEVQSNLTRSLGLKWNSIAG
GNVAASILDSGLSLIFDSTRSLAALNIMATLDALQQQGLSRALRDVNQTVLNNQTARLQSGETFFIRRVVNDQVERVPFD
VGLIVEVTPQITADGQILLNIKAEISGNVQRNPVDGDVDRFTKQVVTTTLRVKDGETVVLGGLTSQESNQSQQGVPLLMD
IPLIGELFKQRTNESTDKELLVVITADILKETASANP

Nucleotide

• Length: 2274 bp

>NTDB_id=92619 QUE39_RS07040 WP_011228639.1 1321310..1323583(+) (pilQ) [Thermus thermophilus strain HB8_001]
ATGAAGAGCGCGTGGATCCGTGCGGCCGTGATCGCCCTCGCAGGGCTAGGGGCTTTTGCCCTTGCGGGGAGCTTTCCCGA
GGAGCCCCGCTTCCAGGCCCCGGTGAACCTCAAGGTCTCGGAATCCCAGGTCAAGGCGGGCCAGACCCTGCCCCTGGACG
TGGTCCTCGAGGCCCTGGCAAGGAGCGTGGGGCTTCAGCCCCTCATCTACCGGGCCTACGACCCAAGCGGGGACCCCGCC
AAGGCCCAGCCCCCCCTGCCCAACATCAAGCTGGACTTCCAGGGCAAGCCCTTCCGGGAGGTCTGGGACCTCCTCTTCGC
CACCTACGGCAACCAGTACAGCCTGGACTACCTCTTCCTGCCCCCCGACGTGGTGGTGGTGGCCCCCACCCAGGTGATCA
CCGCCCTGGTGGACGCCCCGAGCCGCACGGGGGCCATGGAGCGTAGGCCCTACATCGTGGGCATCCCCGAGATCGCCTAC
AAGCGCACGGAGACGGACGCCCAGGGCCAGCCCCGCACGGTCGTCAACATTGAGGGCGCCAAGGCCTGGGTCCAGAACGA
CCTCCTCCCCTTCCTCTCCCGGGAGGCCGCGGGGCTCAACGTGAACTGGATCGTGGTGGAGGAGGGCGGGAGGCTCAAGG
CCGTTCTCTCCGTTCTCGCCACCCCCGAGCAGCACGCCCGCTTCTCCGACATTCTGCAGCGGGCCGGGATTGACTTCCGC
CCCCTCCCCGCCCTGGCCCAGCCCAAGCCCCGGGTGGAGAAGACCTACACCCTCACCTACGCCACCTTCCCCGAACTCCT
CGCCTTCCTCCAGTCCCGCCTCCCCGAGGCCCAGATCAGCGTGGTCCCCACCAACCCCCAAAGGGCCATCGTCCTCGCCA
CCGAGGAGGACCACGCCCGCTTGAGCGAGCTTCTAAAGACGGCCGACGTCCCCAAGACCGTCCGCCGGGTCTACGCCCTG
CAGAACCTCACCTTCGCTGAGGCCCAGGAGCGCCTAAAGCCCCTCCTGGAGAAGGACCTCAAGGGGGCCCGCCTGGAAAG
CCTCCCCGGCAACCCCAAGGCCCTCCTCCTCGAGGCCCCCGAGGCGGAGCACGCCCTCTTCGCCGAGATCCTGAAGGCCC
TGGACGTCCCCCCCCAGGCCCCCCAAGCCCCCCAGGAGGCCACCTTGCGGCGCCTCTACCCCCTGCGCTACGCCAACGCC
GAGCAGGTGGCCCCCTTCCTCGCCCGGGAGGTGCCGGGGATCGTGGTCCAGACCGTTCCCGGCCAGCCCGTCCTCTCGGT
GCGGGGCACGGAGAAGCAGCTCGCCGAGGTGGAAAGCCTCCTCGCCCAGATTGACCGGGCCCCCGAGCAAGGCCCTCCCG
TGTTCCAGCGGGCCTACCAGCTCTCCAACGCCAAGGCGGTGGAGCTCGCCCAGGTGCTCCAGGAGGCGCTCAAGGCCCGG
CAGGCCCAAAACCAGGGCCAGCAGAACCAGGCCCTCCCCACCCGGGAGGCCACGGTGGTGGCCGACCCCAGGACCAACAC
CCTCATCGTCACCGGCACCCAGGAGGACCTCGCCCTGGTGGAGGGCCTCATCCCCAAGCTGGACCAGCCCGTGCCCCAGG
TTTCCTTGCGGGTGCGCATCCAGGAGGTCCAGTCCAACCTGACCCGTAGCCTCGGCCTCAAGTGGAACAGCATCGCCGGC
GGCAACGTGGCGGCCAGCATCCTGGACTCGGGCCTTTCCCTCATCTTTGACTCCACGCGAAGCCTCGCCGCCCTCAACAT
CATGGCCACCCTGGACGCCCTGCAGCAACAGGGCCTCTCCCGGGCCCTCCGGGACGTGAACCAGACCGTCCTCAACAACC
AGACCGCCCGCCTGCAGTCCGGCGAGACCTTCTTCATCCGGCGGGTGGTGAACGACCAGGTGGAACGGGTCCCCTTTGAC
GTGGGGCTCATCGTGGAGGTCACGCCCCAGATCACCGCCGACGGCCAGATCCTCCTCAACATCAAGGCCGAGATCTCGGG
GAACGTCCAGCGCAACCCCGTGGACGGGGACGTGGACCGCTTCACCAAGCAGGTGGTGACCACCACCCTCAGGGTCAAAG
ACGGGGAGACCGTGGTCCTCGGGGGCCTCACCTCCCAGGAGTCCAACCAGAGCCAGCAGGGGGTGCCCCTCCTCATGGAC
ATCCCCCTGATCGGCGAACTCTTCAAGCAGCGGACGAACGAGAGCACCGACAAGGAGCTTTTGGTGGTGATCACCGCCGA
CATCCTCAAGGAGACGGCCTCGGCGAACCCCTAA

Domains

Predicted by InterproScan.

• Bacterial type II/III secretion system short domain (Secretin_N) (457-533)

• Bacterial type II/III secretion system short domain (Secretin_N) (391-450)

• Bacterial type II and III secretion system protein (Secretin) (593-750)

3D structure

• AlphaFold DB: Q5SII6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Thermus thermophilus HB27	98.018	100	0.98