Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   VQZ30_RS20665 Genome accession   NZ_CP142375
Coordinates   4339331..4341346 (+) Length   671 a.a.
NCBI ID   WP_045358352.1    Uniprot ID   -
Organism   Microcystis aeruginosa 1339     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4334331..4346346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VQZ30_RS20645 (VQZ30_20645) - 4334371..4335213 (-) 843 WP_190380524.1 methyltransferase domain-containing protein -
  VQZ30_RS20650 (VQZ30_20650) - 4335421..4336626 (+) 1206 WP_190380525.1 FAD-dependent hydroxylase -
  VQZ30_RS20660 (VQZ30_20660) - 4336923..4338845 (+) 1923 WP_045358351.1 S9 family peptidase -
  VQZ30_RS20665 (VQZ30_20665) pilB/pilB1 4339331..4341346 (+) 2016 WP_045358352.1 GspE/PulE family protein Machinery gene
  VQZ30_RS20670 (VQZ30_20670) pilT 4341520..4342623 (+) 1104 WP_045358353.1 type IV pilus twitching motility protein PilT Machinery gene
  VQZ30_RS20675 (VQZ30_20675) - 4342811..4344025 (+) 1215 WP_045358354.1 type II secretion system F family protein -
  VQZ30_RS20680 (VQZ30_20680) - 4344188..4344809 (+) 622 Protein_4092 ISKra4 family transposase -
  VQZ30_RS20685 (VQZ30_20685) - 4345180..4346121 (+) 942 Protein_4093 ISKra4 family transposase -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 74900.82 Da        Isoelectric Point: 5.4405

>NTDB_id=925540 VQZ30_RS20665 WP_045358352.1 4339331..4341346(+) (pilB/pilB1) [Microcystis aeruginosa 1339]
MTFSSKKTRAVALPNYLSPFGNKLVQSGYIGAEQMQQALVETRKSGRSLVEILKQLTGRPLPPDLQRQHKKNQLFELKIL
YGVESIDAELSDVDGLEIARLIDSLIPIDLCRRYRLIPLGQIKGEATTILIAMVDPDNLEASDDINRILRPRELGIQRLV
ITSEDYERLLEKFHQAQSQLEQEKARLEKEKELEKLSDITDIVGTIDVLSNVPNDEVADDLGVGDANQAPVINLVNKILA
KALQEGTSDIHIEPQEEFLKIRFRRDGVLYQAFEPLPKKITPSVTARFKIMADLDIAERRLPQDGKIRRMYQGRKVDFRV
NTLPSRYGEKVCLRILDNSATQLGLDKLITDQNTLAIVRELASRPFGLLLVTGPTGSGKSTSLYSVLAERNHPGVNISTA
EDPIEYSLPGITQVQVIREKGMDFASILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLD
EMGVEPFMISGSLLGVLAQRLMRRVCTECRVAYHPSQEELARFGLAAANEREVTFYKANTLTIEEIQQARDQGNLCTKCG
GSGYKGRVGVYEVMQNSERLQQLINQGATTDRIKEAAVDEGMVTLLAYSLNLVREGYTTLEEVERVTFTDSGLEAELKAK
RKSGLTCRGCRAELQPEWLDCPYCMTPRFSD

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=925540 VQZ30_RS20665 WP_045358352.1 4339331..4341346(+) (pilB/pilB1) [Microcystis aeruginosa 1339]
ATGACATTTTCTTCCAAGAAAACTCGTGCCGTTGCCCTGCCCAATTACCTTTCCCCCTTTGGTAATAAGTTGGTGCAGTC
GGGCTATATCGGTGCCGAGCAAATGCAACAGGCCCTAGTGGAAACACGGAAATCGGGACGATCGCTAGTAGAAATATTAA
AACAACTAACCGGGCGCCCTTTGCCCCCGGATTTGCAGCGACAGCATAAGAAAAACCAGTTGTTTGAATTAAAAATTCTC
TACGGAGTTGAATCGATCGACGCCGAGTTGAGCGATGTGGATGGGCTGGAAATAGCCCGGTTGATCGATTCTTTGATTCC
CATCGATCTTTGTCGTCGCTATCGTCTCATCCCCCTGGGACAGATCAAAGGAGAGGCAACTACGATTTTGATAGCCATGG
TCGATCCTGACAACCTAGAGGCTAGCGACGATATCAATCGTATTCTCCGCCCCAGGGAGTTAGGTATACAGCGTTTAGTG
ATTACCAGCGAGGACTATGAAAGATTACTGGAAAAATTCCACCAGGCCCAATCGCAACTAGAACAGGAAAAAGCCCGACT
CGAAAAAGAAAAAGAACTGGAAAAACTCTCGGATATCACCGACATTGTCGGCACTATCGATGTGCTCAGCAATGTCCCTA
ACGATGAAGTGGCCGATGATCTCGGGGTGGGGGACGCTAATCAAGCCCCCGTCATCAACCTGGTCAATAAAATTCTAGCT
AAGGCCCTGCAGGAAGGCACTTCTGATATTCACATCGAACCCCAAGAAGAATTCCTGAAAATTCGCTTTCGCAGAGATGG
AGTTCTTTATCAAGCTTTTGAGCCGCTGCCGAAAAAAATTACCCCGTCGGTCACTGCCCGTTTTAAAATCATGGCCGATC
TCGATATCGCCGAACGTCGTCTTCCCCAAGATGGTAAAATTCGGCGGATGTATCAAGGGCGGAAAGTGGATTTTCGGGTT
AATACCCTGCCCAGTCGCTATGGCGAAAAAGTCTGTTTGCGGATTCTCGATAACTCAGCTACCCAGTTGGGTTTAGATAA
ACTGATCACCGATCAAAATACCCTGGCAATTGTCCGGGAATTGGCCAGTCGTCCCTTTGGTCTGTTGCTGGTTACAGGTC
CGACCGGTTCGGGGAAATCCACCAGTTTATACTCGGTTTTAGCCGAAAGAAATCACCCCGGAGTTAATATTAGTACAGCC
GAAGACCCGATCGAATATTCTCTACCAGGCATCACCCAGGTACAGGTAATTCGCGAAAAAGGCATGGATTTCGCCTCGAT
TCTCCGGGCCTTCATGCGACAAGACCCGGATGTGATTCTGGTGGGAGAAACTAGGGACAAGGAGACGGCCAAAACAGCGA
TCGAGGCGGCCTTAACTGGTCACTTGGTCTTAACTACCCTACACACTAACGATGCCGCAGGTGCGATCGCTCGCCTCGAT
GAAATGGGGGTTGAACCGTTTATGATTTCCGGTTCTCTCTTGGGAGTCTTGGCCCAGCGTCTGATGCGGCGCGTCTGTAC
CGAGTGTCGAGTCGCCTATCACCCCAGCCAAGAGGAATTAGCCCGTTTTGGTCTTGCCGCTGCCAATGAACGGGAAGTGA
CCTTTTATAAGGCTAATACCCTAACCATAGAGGAAATTCAACAGGCGCGAGATCAAGGTAATCTTTGTACCAAATGTGGT
GGCAGCGGTTATAAAGGTCGCGTCGGGGTCTATGAGGTAATGCAAAATAGTGAACGACTGCAGCAGTTAATTAACCAAGG
AGCAACCACCGATCGCATTAAGGAGGCGGCCGTCGATGAGGGCATGGTGACACTCCTAGCTTACAGTTTAAATCTGGTGC
GGGAGGGTTACACCACCCTCGAAGAAGTGGAACGGGTGACATTTACCGATTCTGGTCTGGAAGCGGAATTAAAAGCCAAA
CGGAAAAGCGGTTTAACCTGTCGTGGTTGTCGCGCCGAACTACAACCAGAATGGCTCGATTGTCCCTATTGTATGACACC
ACGATTTAGTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-178)

Type II/IV secretion system protein (T2SSE)

(237-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

73.263

98.659

0.723