Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OCV24_RS01265 Genome accession   NZ_AP025497
Coordinates   284006..285031 (+) Length   341 a.a.
NCBI ID   WP_150878778.1    Uniprot ID   -
Organism   Vibrio kanaloae strain LMG 20539     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 279006..290031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV24_RS01250 - 279356..280084 (-) 729 WP_017055839.1 glutathione peroxidase -
  OCV24_RS01255 oxyR 280304..281191 (+) 888 WP_137008133.1 DNA-binding transcriptional regulator OxyR -
  OCV24_RS01260 - 281293..283854 (-) 2562 WP_167514273.1 penicillin-binding protein 1A -
  OCV24_RS01265 pilM 284006..285031 (+) 1026 WP_150878778.1 type IV pilus assembly protein PilM Machinery gene
  OCV24_RS01270 pilN 285015..285614 (+) 600 WP_136997910.1 PilN domain-containing protein Machinery gene
  OCV24_RS01275 pilO 285607..286218 (+) 612 WP_060468742.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV24_RS01280 pilP 286205..286723 (+) 519 WP_146450308.1 pilus assembly protein PilP Machinery gene
  OCV24_RS01285 pilQ 286783..288474 (+) 1692 WP_208806551.1 type IV pilus secretin PilQ Machinery gene
  OCV24_RS01290 aroK 288651..289169 (+) 519 WP_017061125.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38066.71 Da        Isoelectric Point: 7.2623

>NTDB_id=92128 OCV24_RS01265 WP_150878778.1 284006..285031(+) (pilM) [Vibrio kanaloae strain LMG 20539]
MGSSLITGIDINHHSIKAVVLKPVGELYAIVGYKELPISDDIFTANHTLEYQKTVKKLKELKKGLPFGSRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRANALSKAGFKP
IVVDTQAHGLLNIWQLASHLYPEKKNWLLVDVGIDQTSLGIIPQNSAPFYKDIAFGIQELLGAGNPSDMEGVFGTTQDTH
RFIVNLIEKLKRQLQLYSSVNALQPISGIWLMGEGASMPMVVEELERHFQMSCESLNPMSLFENKVAQKRRSSLNCQHFG
IAAGMAMRGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=92128 OCV24_RS01265 WP_150878778.1 284006..285031(+) (pilM) [Vibrio kanaloae strain LMG 20539]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGTATCAAAGCTGTGGTACTAAAACCCGTTGGGGAGTT
GTATGCCATCGTGGGATACAAAGAGCTGCCTATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAAGAAAGGACTGCCTTTTGGAAGTCGTAACGTCGCTATTTCAGTACCAGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGATAGAGAGAAAGAGTTTTCTATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAAGAGCTGAGTTTAGATTTTGTAAAGCTCGAAGACAAACGGTTTGGTAAAGGATCGACAA
GTAGTTATCAGGTGTACGCGACTCGCAAGGAAGTTGTGGATAGTCGAGCCAATGCATTAAGCAAAGCCGGGTTTAAACCT
ATCGTCGTTGATACCCAAGCACATGGGCTTCTCAATATTTGGCAATTGGCTTCGCATTTGTACCCAGAAAAGAAGAACTG
GCTGCTGGTGGACGTTGGAATCGACCAAACCTCGTTAGGGATTATCCCGCAGAATTCTGCGCCTTTTTATAAAGACATCG
CCTTTGGTATTCAAGAGCTGTTAGGCGCAGGCAATCCAAGTGATATGGAAGGCGTGTTCGGCACCACTCAGGATACACAT
CGGTTTATTGTTAATCTTATTGAAAAGCTCAAGCGTCAACTGCAGCTCTATTCGTCGGTCAATGCGCTTCAACCTATCTC
AGGGATTTGGTTAATGGGTGAAGGAGCGAGCATGCCAATGGTCGTTGAGGAGTTAGAACGTCATTTTCAGATGAGTTGTG
AGTCACTGAACCCTATGTCTTTGTTTGAAAATAAGGTGGCCCAGAAACGCCGATCGTCACTGAATTGTCAGCACTTTGGT
ATTGCGGCGGGGATGGCAATGCGTGGTCTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-219)

Type IV pilus assembly protein PilM; (PilM_2)

(242-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

53.372

100

0.534
  pilM Vibrio campbellii strain DS40M4

46.491

100

0.466

Multiple sequence alignment