Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   VFH86_RS01300 Genome accession   NZ_CP142014
Coordinates   279149..279733 (+) Length   194 a.a.
NCBI ID   WP_000902745.1    Uniprot ID   A0A0H6BVQ2
Organism   Vibrio cholerae strain LK-18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 274149..284733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VFH86_RS01285 (VFH86_01285) oxyR 274555..275448 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  VFH86_RS01290 (VFH86_01290) - 275523..278000 (-) 2478 WP_032474776.1 penicillin-binding protein 1A -
  VFH86_RS01295 (VFH86_01295) pilM 278143..279159 (+) 1017 WP_345658103.1 type IV pilus assembly protein PilM Machinery gene
  VFH86_RS01300 (VFH86_01300) pilN 279149..279733 (+) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  VFH86_RS01305 (VFH86_01305) pilO 279726..280316 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  VFH86_RS01310 (VFH86_01310) pilP 280306..280824 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  VFH86_RS01315 (VFH86_01315) pilQ 280850..282565 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ family protein Machinery gene
  VFH86_RS01320 (VFH86_01320) aroK 282752..283276 (+) 525 WP_000818616.1 shikimate kinase AroK -
  VFH86_RS01325 (VFH86_01325) aroB 283304..284389 (+) 1086 WP_000439805.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 21964.42 Da        Isoelectric Point: 9.9503

>NTDB_id=920921 VFH86_RS01300 WP_000902745.1 279149..279733(+) (pilN) [Vibrio cholerae strain LK-18]
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEITGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQILTPASNPQAGGAHNG

Nucleotide


Download         Length: 585 bp        

>NTDB_id=920921 VFH86_RS01300 WP_000902745.1 279149..279733(+) (pilN) [Vibrio cholerae strain LK-18]
ATGTTGCATAAGGTCAACTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTACTGGCGGTGTTGATGCAATTTGCCGCAGGCGAATACTTGGGCGGACAAATGGCTTTGCAGCAAGAGC
GGATTGGTTATTTGCAACAACATATTTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTGCAGCAAAAGCGCAATAAAACGACTGAGTTTATGAACCAAATGCC
CAACCTTATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATAACCGGGATTAGCGATT
CCACCGCTCGCTTGGCAACCATGCTCGATAACTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAAATTTTAACCCCAGCCTCTAACCCGCA
AGCGGGAGGTGCACACAATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H6BVQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

100

100

1
  pilN Vibrio campbellii strain DS40M4

60.452

91.237

0.552