Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VFH86_RS01295 Genome accession   NZ_CP142014
Coordinates   278143..279159 (+) Length   338 a.a.
NCBI ID   WP_345658103.1    Uniprot ID   -
Organism   Vibrio cholerae strain LK-18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 273143..284159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VFH86_RS01280 (VFH86_01280) - 273684..274415 (-) 732 WP_176398377.1 glutathione peroxidase -
  VFH86_RS01285 (VFH86_01285) oxyR 274555..275448 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  VFH86_RS01290 (VFH86_01290) - 275523..278000 (-) 2478 WP_032474776.1 penicillin-binding protein 1A -
  VFH86_RS01295 (VFH86_01295) pilM 278143..279159 (+) 1017 WP_345658103.1 type IV pilus assembly protein PilM Machinery gene
  VFH86_RS01300 (VFH86_01300) pilN 279149..279733 (+) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  VFH86_RS01305 (VFH86_01305) pilO 279726..280316 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  VFH86_RS01310 (VFH86_01310) pilP 280306..280824 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  VFH86_RS01315 (VFH86_01315) pilQ 280850..282565 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ family protein Machinery gene
  VFH86_RS01320 (VFH86_01320) aroK 282752..283276 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37808.54 Da        Isoelectric Point: 7.3328

>NTDB_id=920920 VFH86_RS01295 WP_345658103.1 278143..279159(+) (pilM) [Vibrio cholerae strain LK-18]
MGKSLVTGIDIGHHSIKAVVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRWHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=920920 VFH86_RS01295 WP_345658103.1 278143..279159(+) (pilM) [Vibrio cholerae strain LK-18]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGTGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTGGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCAGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCTGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.408

100

0.994
  pilM Vibrio campbellii strain DS40M4

48.817

100

0.488