Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   VN992_RS06675 Genome accession   NZ_CP141949
Coordinates   1464425..1464973 (-) Length   182 a.a.
NCBI ID   WP_370427031.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain AH2022     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1459425..1469973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VN992_RS06660 (VN992_06650) - 1459919..1460440 (+) 522 WP_005598290.1 SprT family zinc-dependent metalloprotease -
  VN992_RS06665 (VN992_06655) - 1460545..1461831 (+) 1287 WP_005601799.1 NAD(P)/FAD-dependent oxidoreductase -
  VN992_RS06670 (VN992_06660) - 1462023..1464350 (-) 2328 WP_039709241.1 Tex family protein -
  VN992_RS06675 (VN992_06665) pilF 1464425..1464973 (-) 549 WP_370427031.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  VN992_RS06680 (VN992_06670) - 1465027..1466208 (-) 1182 WP_005605050.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  VN992_RS06705 (VN992_06695) gltX 1467113..1468552 (+) 1440 WP_005608501.1 glutamate--tRNA ligase -
  VN992_RS06715 (VN992_06705) yefM 1468890..1469141 (+) 252 WP_005598318.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  VN992_RS06720 (VN992_06710) - 1469138..1469398 (+) 261 WP_370427032.1 Txe/YoeB family addiction module toxin -
  VN992_RS06725 (VN992_06715) - 1469557..1469724 (-) 168 WP_005598320.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20603.19 Da        Isoelectric Point: 6.9479

>NTDB_id=920575 VN992_RS06675 WP_370427031.1 1464425..1464973(-) (pilF) [Actinobacillus pleuropneumoniae strain AH2022]
MNLAKFLRNLSACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKSSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKANEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=920575 VN992_RS06675 WP_370427031.1 1464425..1464973(-) (pilF) [Actinobacillus pleuropneumoniae strain AH2022]
ATGAATCTTGCAAAATTTTTGCGAAATTTGAGCGCTTGTATGCTGTTATCCGTGTTGTTCGGTTGTTCTTCTCAGCTTCC
AACCTCCCAAACTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGTATTAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTATTTGCCTCACTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCATGCGGAACAGGCTTATCAGCAAGCATTGAAATCAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGAACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGCAAATGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

65.957

100

0.681
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44