Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   VK748_RS05355 Genome accession   NZ_CP141948
Coordinates   1111924..1113513 (+) Length   529 a.a.
NCBI ID   WP_043273654.1    Uniprot ID   A0A1A5D6Q3
Organism   Pseudomonas citronellolis strain G5.41a     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1106924..1118513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VK748_RS05335 (VK748_05335) pgeF 1108718..1109446 (-) 729 WP_324710587.1 peptidoglycan editing factor PgeF -
  VK748_RS05340 (VK748_05340) rluD 1109443..1110402 (-) 960 WP_324710588.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  VK748_RS05345 (VK748_05345) - 1110549..1111562 (+) 1014 WP_009617585.1 outer membrane protein assembly factor BamD -
  VK748_RS05350 (VK748_05350) - 1111701..1111931 (+) 231 WP_009617584.1 PP0621 family protein -
  VK748_RS05355 (VK748_05355) pilS 1111924..1113513 (+) 1590 WP_043273654.1 sensor histidine kinase Regulator
  VK748_RS05360 (VK748_05360) pilR 1113525..1114862 (+) 1338 WP_061560713.1 sigma-54-dependent transcriptional regulator Regulator
  VK748_RS05365 (VK748_05365) thiO 1114907..1116016 (-) 1110 WP_061560712.1 glycine oxidase ThiO -
  VK748_RS05370 (VK748_05370) - 1116195..1116707 (+) 513 WP_324710589.1 GspH/FimT family protein -
  VK748_RS05375 (VK748_05375) - 1116857..1117336 (+) 480 WP_061560711.1 GspH/FimT family pseudopilin -
  VK748_RS05380 (VK748_05380) pilV 1117333..1117887 (+) 555 WP_061560710.1 type IV pilus modification protein PilV -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58463.49 Da        Isoelectric Point: 7.2738

>NTDB_id=920538 VK748_RS05355 WP_043273654.1 1111924..1113513(+) (pilS) [Pseudomonas citronellolis strain G5.41a]
MAKAIPISGQQGQRILRLYHLYRLSIGLLLVLLISSDLDDRVLNVGQSALFHASCWSYLIVNILIAVLMPTPRQLLPIFL
LALFDILALCGLFYAAGGVPSGIGNLLVVAVGIANILLRGRIGLLIAAVAAIGMIYLTFYLSLSQPTAINHYVQAGSLGA
LSFAAALLLQGLARRQQLTEHLAEERATTVANLEELNALILQRMRTGILVLDSRHRVLLANQSALGLLAQPELAGKLATE
HCPELLDSLQQWERNPSLRPPSLQAPEGGPMLQPSFIPLRRGEEQHTLVFLEDISQIAQQAQQLKLAALGRLTAGIAHEI
RNPLGAISHAAQLLQESETLDAPDRRLTQIIQDQSRRMNLVIENVLQLSRRRQAEPQQLDLRQWLQRFTDEQREGLRGDQ
QIHLQMGGGEIQTRMDPSQLTQVLGNLVQNGLRYSARQNGSGQVWLNLFRDRDSDLPVLEVLDDGPGIAPEQLGKLFEPF
FTTESKGTGLGLYISRELCESNQARIDFKNRDEGGGSFRITFAHPRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=920538 VK748_RS05355 WP_043273654.1 1111924..1113513(+) (pilS) [Pseudomonas citronellolis strain G5.41a]
ATGGCTAAGGCCATCCCCATCAGCGGCCAGCAGGGGCAGCGCATCCTCCGCCTGTACCACCTGTACCGCCTGAGCATCGG
CCTGCTGCTGGTGCTGCTGATTTCCAGCGACCTCGACGACCGCGTGCTCAACGTCGGCCAGTCGGCGCTGTTCCATGCCA
GCTGCTGGAGCTACCTGATCGTCAACATCCTGATCGCCGTGCTGATGCCGACGCCGCGCCAGTTGCTGCCGATCTTCCTC
CTGGCGCTGTTCGACATCCTCGCGCTATGCGGCCTGTTCTACGCCGCCGGCGGCGTGCCCAGCGGCATCGGCAACCTGCT
GGTGGTGGCGGTGGGCATCGCCAACATCCTGCTGCGCGGACGCATCGGCCTGCTGATCGCCGCCGTCGCCGCGATCGGCA
TGATCTACCTGACCTTCTACCTCAGCCTCAGCCAGCCCACGGCGATCAACCACTACGTCCAGGCCGGCAGCCTCGGCGCC
CTGAGCTTCGCCGCCGCGCTGCTGCTGCAGGGCCTGGCGCGGCGCCAGCAACTCACCGAGCACCTGGCCGAGGAACGCGC
CACCACCGTGGCCAACCTCGAAGAACTCAACGCCCTCATCCTGCAGCGCATGCGCACCGGCATCCTGGTGCTGGACAGCC
GGCACCGAGTGCTGCTGGCCAACCAGAGCGCCCTGGGCCTGCTGGCCCAGCCGGAACTGGCCGGCAAGCTCGCCACCGAG
CATTGCCCGGAACTGCTCGACAGCCTGCAGCAGTGGGAACGCAACCCCTCGCTGCGCCCGCCCAGCCTGCAGGCGCCCGA
AGGCGGCCCCATGCTGCAGCCGAGCTTCATCCCGCTGCGCCGCGGCGAGGAACAGCACACCCTGGTGTTCCTCGAAGACA
TCTCGCAGATCGCCCAGCAGGCCCAGCAGCTCAAGCTGGCGGCCCTGGGCCGGCTGACCGCCGGCATCGCCCACGAGATC
CGCAACCCCCTGGGCGCCATCAGCCACGCCGCGCAGCTGCTGCAGGAGTCCGAAACGCTCGACGCCCCCGACCGGCGCCT
GACGCAGATCATCCAGGACCAGTCGCGACGCATGAACCTGGTGATCGAGAACGTCCTGCAACTGTCGCGGCGACGCCAGG
CCGAACCGCAACAGCTGGACCTCAGGCAGTGGCTGCAACGCTTCACCGACGAGCAGCGCGAAGGCCTGCGCGGCGACCAG
CAGATCCACCTGCAAATGGGCGGCGGCGAGATCCAGACGCGCATGGACCCCAGCCAACTCACCCAGGTGCTGGGTAACCT
GGTGCAGAATGGGTTACGGTACAGTGCCCGGCAGAACGGCAGCGGCCAGGTGTGGTTGAACCTGTTCCGCGACCGGGACA
GCGACCTGCCGGTGCTGGAGGTGCTCGACGACGGCCCCGGCATCGCCCCGGAGCAGCTGGGCAAGCTGTTCGAACCCTTC
TTCACCACCGAGAGCAAGGGCACCGGGCTGGGCCTGTACATTTCCCGCGAGCTGTGCGAAAGCAACCAGGCCCGCATCGA
CTTCAAGAACCGCGACGAAGGCGGTGGCAGCTTCCGCATCACCTTCGCCCACCCGCGCAAGCAGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(308-372)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(416-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1A5D6Q3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

73.295

99.811

0.732