Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AB3N43_RS17780 Genome accession   NZ_CP162163
Coordinates   3627779..3629212 (-) Length   477 a.a.
NCBI ID   WP_406811564.1    Uniprot ID   -
Organism   Escherichia coli strain STS63-1-E     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3622779..3634212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3N43_RS17745 (AB3N43_17735) elbB 3623470..3624123 (+) 654 WP_038976814.1 isoprenoid biosynthesis glyoxalase ElbB -
  AB3N43_RS17750 (AB3N43_17740) mtgA 3624120..3624848 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  AB3N43_RS17755 (AB3N43_17745) yrbL 3624845..3625477 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  AB3N43_RS17760 (AB3N43_17750) npr 3625691..3625963 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  AB3N43_RS17765 (AB3N43_17755) rapZ 3625960..3626814 (-) 855 WP_000243741.1 RNase adapter RapZ -
  AB3N43_RS17770 (AB3N43_17760) ptsN 3626860..3627351 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AB3N43_RS17775 (AB3N43_17765) hpf 3627469..3627756 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  AB3N43_RS17780 (AB3N43_17770) rpoN/rpoN1 3627779..3629212 (-) 1434 WP_406811564.1 RNA polymerase factor sigma-54 Machinery gene
  AB3N43_RS17785 (AB3N43_17775) lptB 3629260..3629985 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  AB3N43_RS17790 (AB3N43_17780) lptA 3629992..3630549 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  AB3N43_RS17795 (AB3N43_17785) lptC 3630518..3631093 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  AB3N43_RS17800 (AB3N43_17790) kdsC 3631090..3631656 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AB3N43_RS17805 (AB3N43_17795) kdsD 3631677..3632663 (-) 987 WP_276384439.1 arabinose-5-phosphate isomerase KdsD -
  AB3N43_RS17810 (AB3N43_17800) yrbG 3632677..3633654 (-) 978 WP_000922901.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53992.81 Da        Isoelectric Point: 4.3837

>NTDB_id=920437 AB3N43_RS17780 WP_406811564.1 3627779..3629212(-) (rpoN/rpoN1) [Escherichia coli strain STS63-1-E]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVNDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=920437 AB3N43_RS17780 WP_406811564.1 3627779..3629212(-) (rpoN/rpoN1) [Escherichia coli strain STS63-1-E]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTAATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTACTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.024

100

0.463