Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   VDS57_RS11660 Genome accession   NZ_CP141839
Coordinates   2189005..2189454 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain PN1236     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2184005..2194454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VDS57_RS11620 (VDS57_11620) - 2184330..2184527 (+) 198 WP_121509395.1 hypothetical protein -
  VDS57_RS11625 (VDS57_11625) yckC 2184547..2185002 (+) 456 WP_014478793.1 RDD family protein -
  VDS57_RS11630 (VDS57_11630) yckD 2185084..2185416 (+) 333 WP_014478794.1 YckD family protein -
  VDS57_RS11635 (VDS57_11635) - 2185544..2185966 (+) 423 WP_014478795.1 MarR family winged helix-turn-helix transcriptional regulator -
  VDS57_RS11640 (VDS57_11640) - 2185954..2186274 (+) 321 WP_014478796.1 DUF3147 family protein -
  VDS57_RS11645 (VDS57_11645) - 2186293..2187037 (+) 745 Protein_2245 AAA family ATPase -
  VDS57_RS11650 (VDS57_11650) bglC 2187110..2188543 (+) 1434 WP_014478799.1 6-phospho-beta-glucosidase -
  VDS57_RS11655 (VDS57_11655) nin/comJ 2188580..2188978 (-) 399 WP_014478800.1 DNA-entry nuclease inhibitor Regulator
  VDS57_RS11660 (VDS57_11660) nucA/comI 2189005..2189454 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  VDS57_RS11665 (VDS57_11665) tlpC 2189622..2191343 (-) 1722 Protein_2249 methyl-accepting chemotaxis protein TlpC -
  VDS57_RS11670 (VDS57_11670) hxlB 2191454..2192011 (-) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  VDS57_RS11675 (VDS57_11675) hxlA 2192017..2192649 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  VDS57_RS11680 (VDS57_11680) hxlR 2192878..2193240 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=920156 VDS57_RS11660 WP_014475770.1 2189005..2189454(-) (nucA/comI) [Bacillus subtilis strain PN1236]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=920156 VDS57_RS11660 WP_014475770.1 2189005..2189454(-) (nucA/comI) [Bacillus subtilis strain PN1236]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987