Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VCJ09_RS21350 Genome accession   NZ_CP141799
Coordinates   4642463..4643527 (-) Length   354 a.a.
NCBI ID   WP_079203425.1    Uniprot ID   -
Organism   Pseudomonas paeninsulae strain IT1137     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4637463..4648527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCJ09_RS21325 aroK 4638090..4638608 (-) 519 WP_324732031.1 shikimate kinase AroK -
  VCJ09_RS21330 pilQ 4638614..4640689 (-) 2076 WP_324734710.1 type IV pilus secretin PilQ Machinery gene
  VCJ09_RS21335 pilP 4640744..4641271 (-) 528 WP_324732032.1 type 4a pilus biogenesis lipoprotein PilP -
  VCJ09_RS21340 pilO 4641268..4641891 (-) 624 WP_324732033.1 type 4a pilus biogenesis protein PilO -
  VCJ09_RS21345 pilN 4641888..4642463 (-) 576 WP_324732034.1 type 4a pilus biogenesis protein PilN -
  VCJ09_RS21350 pilM 4642463..4643527 (-) 1065 WP_079203425.1 pilus assembly protein PilM Machinery gene
  VCJ09_RS21355 - 4643709..4646138 (+) 2430 WP_324732035.1 penicillin-binding protein 1A -
  VCJ09_RS21360 - 4646424..4647692 (-) 1269 WP_079203427.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37874.20 Da        Isoelectric Point: 4.4526

>NTDB_id=919563 VCJ09_RS21350 WP_079203425.1 4642463..4643527(-) (pilM) [Pseudomonas paeninsulae strain IT1137]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALARVLAKAKTGVKSVAV
AVAGSAVITKTIEMEAGLSDDDLENQLKVEADQYIPYPLEEVAIDFEVQGSSPRNPDRVEVLLAACRKENVEVREAALAL
AGLVAKVVDVEAYALERAYGLLTEQLGSGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSRVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=919563 VCJ09_RS21350 WP_079203425.1 4642463..4643527(-) (pilM) [Pseudomonas paeninsulae strain IT1137]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATACGCTGTTGGGGATCGATATCAGTTCGACCTCCGTCAAGCTCCTCGAATT
GAGTCGCTCAGGAAGCCGCTACAAGGTAGAGGCTTACGCTGTAGAGCCGCTCCCACCAAATGCGGTGGTCGAGAAAAATA
TTGCCGAACTTGAGGGGGTCGGGCAGGCGCTTGCGCGTGTGCTGGCCAAAGCCAAGACCGGTGTAAAATCCGTAGCGGTC
GCTGTAGCTGGCTCCGCGGTGATTACCAAGACCATCGAGATGGAAGCCGGGCTTTCTGATGATGATCTCGAGAATCAACT
GAAGGTCGAGGCCGATCAGTACATTCCATATCCGCTCGAAGAAGTGGCTATTGATTTCGAGGTGCAAGGCTCTTCGCCGC
GGAATCCTGATCGGGTCGAGGTGCTTCTGGCAGCCTGCCGCAAGGAAAACGTAGAGGTTCGCGAGGCCGCCCTGGCCTTG
GCCGGGTTGGTCGCCAAAGTGGTCGATGTCGAAGCCTACGCCCTGGAGCGTGCTTATGGCTTGCTCACGGAGCAGCTTGG
TAGCGGGCATGACGAGCTGACGGTGGCCGTGGTCGATATTGGTGCAACCATGACCACGCTCAGCGTGTTGCACAATGGCC
GCACTATTTATACGCGTGAGCAGCTATTTGGCGGCAAGCAGCTCACCGAGGAAATCCAGCGGCGTTACGGCCTATCCGTG
GAGGAAGCCGGTCTTGCGAAAAAACAGGGCGGCCTTCCCGATGACTACGACAGCGAAGTATTACAGCCGTTCAAGGACGC
GGTCGTACAACAAGTTTCGCGCTCCCTGCAATTCTTCTTTGCTGCAGGTCAGTTCAATGATGTCGATTACATTCTTCTGG
CTGGCGGCACGGCTTCCATTCCCGACCTCGATCGTCTGATTCAGCAAAAGATCGGCACGCAAACCCTGGTTGCCAATCCT
TTCGCGGACATGGCACTGAGCAGCAGGGTCAATGCTGGAGCGTTGGCCAGTGATGCCCCAGCATTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTCGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

52.825

100

0.528
  pilM Legionella pneumophila strain ERS1305867

46.197

100

0.463