Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   U2H22_RS01770 Genome accession   NZ_CP141265
Coordinates   329438..329875 (-) Length   145 a.a.
NCBI ID   WP_218791482.1    Uniprot ID   -
Organism   Bacillus velezensis strain L2D39     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 324438..334875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U2H22_RS01745 (U2H22_01745) - 325457..326593 (+) 1137 WP_025284207.1 zinc-dependent alcohol dehydrogenase -
  U2H22_RS01750 (U2H22_01750) - 326608..327042 (+) 435 WP_059366555.1 RDD family protein -
  U2H22_RS01755 (U2H22_01755) - 327115..327438 (+) 324 WP_007409357.1 YckD family protein -
  U2H22_RS01760 (U2H22_01760) - 327542..328978 (+) 1437 WP_072588782.1 family 1 glycosylhydrolase -
  U2H22_RS01765 (U2H22_01765) nin/comJ 329019..329417 (-) 399 WP_218791481.1 competence protein ComJ Regulator
  U2H22_RS01770 (U2H22_01770) nucA/comI 329438..329875 (-) 438 WP_218791482.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  U2H22_RS01775 (U2H22_01775) hxlB 330232..330789 (-) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  U2H22_RS01780 (U2H22_01780) hxlA 330786..331421 (-) 636 WP_012116769.1 3-hexulose-6-phosphate synthase -
  U2H22_RS01785 (U2H22_01785) - 331653..332015 (+) 363 WP_012116770.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16042.33 Da        Isoelectric Point: 8.4850

>NTDB_id=916632 U2H22_RS01770 WP_218791482.1 329438..329875(-) (nucA/comI) [Bacillus velezensis strain L2D39]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQAVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=916632 U2H22_RS01770 WP_218791482.1 329438..329875(-) (nucA/comI) [Bacillus velezensis strain L2D39]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCCGATAACGAGCAGGCCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.016

86.897

0.634