Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   VKI21_RS13205 Genome accession   NZ_CP141255
Coordinates   3017674..3019695 (-) Length   673 a.a.
NCBI ID   WP_324283120.1    Uniprot ID   -
Organism   Cyanobacterium aponinum UTEX 3222     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3012674..3024695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VKI21_RS13180 (VKI21_13180) - 3012693..3013199 (+) 507 WP_015218765.1 cupin domain-containing protein -
  VKI21_RS13185 (VKI21_13185) - 3013766..3014542 (+) 777 WP_155084024.1 DUF1868 domain-containing protein -
  VKI21_RS13190 (VKI21_13190) - 3014551..3015159 (+) 609 WP_099436714.1 precorrin-8X methylmutase -
  VKI21_RS13195 (VKI21_13195) rsmD 3015182..3015730 (+) 549 WP_015218768.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  VKI21_RS13200 (VKI21_13200) - 3016111..3017256 (-) 1146 WP_342596799.1 SpoIIE family protein phosphatase -
  VKI21_RS13205 (VKI21_13205) pilB/pilB1 3017674..3019695 (-) 2022 WP_324283120.1 GspE/PulE family protein Machinery gene
  VKI21_RS13210 (VKI21_13210) - 3019822..3019962 (+) 141 WP_342596800.1 recombinase family protein -
  VKI21_RS13215 (VKI21_13215) - 3020045..3020317 (+) 273 WP_015218771.1 hypothetical protein -
  VKI21_RS13220 (VKI21_13220) - 3020600..3021361 (+) 762 WP_155084028.1 hypothetical protein -
  VKI21_RS13225 (VKI21_13225) - 3021339..3022382 (+) 1044 WP_342596801.1 aromatic ring-hydroxylating dioxygenase subunit alpha -
  VKI21_RS13230 (VKI21_13230) - 3022400..3023314 (+) 915 WP_342596802.1 ferritin-like domain-containing protein -

Sequence

Protein


Download         Length: 673 a.a.        Molecular weight: 75162.10 Da        Isoelectric Point: 4.9555

>NTDB_id=916434 VKI21_RS13205 WP_324283120.1 3017674..3019695(-) (pilB/pilB1) [Cyanobacterium aponinum UTEX 3222]
MSSASKEKLKTALVSPDYFNPFGNKLIQAGYIDKEDLKRALVQVRNTKQPLVKILENITHKALSPELVRQYKQHQLFELK
ILYGVDCVDPESEEIAADQMTDLIESIMGVQVCRRHKVLPLRRIEGEPPVIVVAMVNPDDLQALDDVKKILRAKGLDLQR
IVITEEDFDKLINRFYEEDKQRQKREEELKEKQKLDQLGDVSDIVDDLDQNLDEADEENPDGDLTSNDANQAPIIKLVNK
VLIKALQEGASDIHVEPQETRMRVRLRKDGVLREYFTLPKHIATAVVARFKIMAELDIAERRLPQDGKIRRVFQGRNVDF
RVNCLPSRYGEKVCLRILDNSATQLGLDFLISDEETLAKVRDIASRPFGLILVTGPTGSGKSTTLYSVLAERNDPGVNIS
TAEDPIEYALEGITQVQVIREKGMDFASILRAFLRQDPDIILVGETRDAETAKTAIEAALTGHLVLTTLHTNDAAGAIAR
LDEMGVEPFMISGALLGVLAQRLMRRVCSECKIAYVPTKEELARFGLSVSGEESVTFYKANTLSGEALQQAQANGTVCQK
CGGGGYKGRVGVYEFMVMSEELQKLINRGATTDMIKEKAVEEGMVTILAYSLNLVRQGHTTLEEVERVTFTDSGLEAELK
AKRKSSLTCSGCGAELQQEWLDCPYCMTPRFSE

Nucleotide


Download         Length: 2022 bp        

>NTDB_id=916434 VKI21_RS13205 WP_324283120.1 3017674..3019695(-) (pilB/pilB1) [Cyanobacterium aponinum UTEX 3222]
ATTTCTTCTGCTTCTAAAGAAAAACTAAAAACAGCCTTAGTCAGTCCAGATTATTTCAATCCCTTTGGAAATAAACTAAT
TCAAGCAGGTTACATTGATAAGGAAGATTTAAAAAGGGCGTTGGTACAAGTGAGAAATACTAAACAGCCTTTAGTAAAAA
TCTTAGAAAATATCACCCACAAGGCTTTGTCTCCTGAGTTAGTGCGACAGTACAAACAACATCAACTTTTTGAATTAAAA
ATTCTTTATGGGGTTGATTGTGTTGACCCAGAGTCAGAAGAAATAGCAGCGGATCAAATGACCGATTTAATCGAGTCTAT
TATGGGAGTTCAGGTTTGTCGTCGTCATAAAGTTTTACCCTTGCGCCGCATTGAGGGTGAACCGCCTGTAATTGTTGTTG
CTATGGTTAATCCTGATGATTTACAGGCGTTGGATGATGTCAAAAAAATATTACGAGCCAAAGGCCTTGACTTACAAAGA
ATTGTTATTACTGAAGAAGATTTTGATAAGTTAATTAACAGGTTTTATGAAGAAGATAAGCAAAGACAAAAAAGAGAAGA
GGAGTTAAAAGAAAAGCAAAAATTAGATCAGTTAGGGGATGTTAGTGATATTGTTGATGATTTGGATCAGAATTTAGATG
AAGCTGATGAAGAAAATCCAGATGGTGATTTGACTTCTAATGATGCTAATCAAGCCCCTATTATCAAGTTAGTTAATAAA
GTTCTCATCAAGGCTTTACAAGAAGGGGCATCAGACATTCATGTTGAACCCCAAGAAACTAGAATGAGGGTGCGTTTACG
TAAAGATGGGGTTTTAAGGGAATATTTTACTCTACCTAAACATATTGCGACTGCGGTGGTTGCTCGTTTCAAAATCATGG
CTGAGTTGGATATTGCAGAACGCCGTTTACCTCAAGATGGAAAAATTCGTCGGGTGTTTCAGGGAAGAAATGTGGATTTT
CGAGTTAACTGTTTGCCTAGTCGTTACGGTGAAAAGGTCTGTTTAAGGATATTAGATAATTCGGCTACTCAATTAGGTTT
AGACTTTTTAATCTCGGACGAGGAAACATTGGCAAAAGTTAGAGATATTGCCAGTCGTCCTTTTGGTTTAATTTTGGTTA
CAGGTCCTACAGGTTCGGGTAAGTCCACAACTCTCTATTCTGTCCTAGCGGAGAGAAATGATCCGGGAGTGAACATAAGT
ACGGCGGAAGACCCTATTGAGTATGCTTTAGAAGGAATCACTCAGGTGCAAGTAATCCGAGAAAAAGGTATGGATTTCGC
TTCAATTCTTCGGGCTTTTCTCAGACAAGACCCTGATATTATTCTGGTGGGTGAAACACGGGACGCTGAAACCGCAAAAA
CTGCGATCGAAGCCGCTTTGACTGGACACTTGGTTTTAACCACTCTACATACTAATGATGCGGCAGGTGCGATCGCACGT
TTGGACGAAATGGGGGTTGAACCCTTTATGATTTCTGGTGCATTGTTAGGGGTTTTGGCACAAAGATTAATGCGAAGAGT
TTGTAGTGAGTGTAAAATTGCTTATGTTCCTACTAAAGAAGAATTAGCCCGTTTTGGTTTATCCGTATCTGGAGAAGAAT
CTGTTACCTTTTATAAAGCGAATACTTTGTCAGGAGAAGCCCTACAACAAGCTCAAGCGAATGGCACGGTTTGCCAAAAA
TGTGGTGGCGGTGGTTATAAAGGGCGTGTGGGTGTTTATGAGTTTATGGTTATGAGTGAAGAGTTGCAAAAACTCATTAA
CCGAGGAGCAACCACTGACATGATTAAAGAAAAGGCTGTTGAAGAAGGAATGGTAACGATTTTAGCCTATAGTTTAAATC
TAGTTCGTCAAGGTCATACAACCTTAGAAGAAGTTGAAAGGGTAACATTTACTGATTCTGGTTTGGAAGCAGAATTGAAG
GCTAAACGAAAATCATCTTTAACCTGTAGTGGCTGTGGAGCAGAATTACAACAGGAATGGTTAGATTGTCCTTATTGTAT
GACTCCTCGTTTCAGTGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(240-506)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

68.95

97.623

0.673
  pilF Thermus thermophilus HB27

41.374

93.016

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.968

93.314

0.373