Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   VKI22_RS00985 Genome accession   NZ_CP141254
Coordinates   225938..227974 (+) Length   678 a.a.
NCBI ID   WP_410765596.1    Uniprot ID   -
Organism   Cyanobacterium aponinum UTEX 3221     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 220938..232974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VKI22_RS00960 (VKI22_00960) - 221531..222160 (-) 630 WP_324282405.1 hypothetical protein -
  VKI22_RS00965 (VKI22_00965) - 222328..223242 (-) 915 WP_324282406.1 ferritin-like domain-containing protein -
  VKI22_RS00970 (VKI22_00970) - 223260..224303 (-) 1044 WP_324282407.1 aromatic ring-hydroxylating dioxygenase subunit alpha -
  VKI22_RS00975 (VKI22_00975) - 224281..225042 (-) 762 WP_324282408.1 hypothetical protein -
  VKI22_RS00980 (VKI22_00980) - 225331..225603 (-) 273 WP_324282409.1 hypothetical protein -
  VKI22_RS00985 (VKI22_00985) pilB/pilB1 225938..227974 (+) 2037 WP_410765596.1 GspE/PulE family protein Machinery gene
  VKI22_RS00990 (VKI22_00990) - 228399..229544 (+) 1146 WP_324282410.1 PP2C family protein-serine/threonine phosphatase -
  VKI22_RS00995 (VKI22_00995) rsmD 229917..230465 (-) 549 WP_324282411.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  VKI22_RS01000 (VKI22_01000) - 230488..231096 (-) 609 WP_099436714.1 precorrin-8X methylmutase -
  VKI22_RS01005 (VKI22_01005) - 231105..231881 (-) 777 WP_155084024.1 DUF1868 domain-containing protein -

Sequence

Protein


Download         Length: 678 a.a.        Molecular weight: 75702.72 Da        Isoelectric Point: 4.9555

>NTDB_id=916400 VKI22_RS00985 WP_410765596.1 225938..227974(+) (pilB/pilB1) [Cyanobacterium aponinum UTEX 3221]
MTQTPISSASKEKLKTALVSPDYFNPFGNKLIQAGYIDKEDLKRALVQVRNTKQPLVKILENITHKALSPELVRQYKQHQ
LFELKILYGVDCVDPESEEIAADQMTDLIESIMGVQVCRRHKVLPLRRIEGEPPVIVVAMVNPDDLQALDDVKKILRAKG
LDLQRIVITEEDFDKLINRFYEEDKQRQKREEELKEKQKLDQLGDVSDIVDDLDQNLDEADEENPDGDLTSNDANQAPII
KLVNKVLIKALQEGASDIHVEPQETRMRVRLRKDGVLREYFTLPKHIATAVVARFKIMAELDIAERRLPQDGKIRRVFQG
RNVDFRVNCLPSRYGEKVCLRILDNSATQLGLDFLISDEETLAKVRDIASRPFGLILVTGPTGSGKSTTLYSVLAERNDP
GVNISTAEDPIEYALEGITQVQVIREKGMDFASILRAFLRQDPDIILVGETRDAETAKTAIEAALTGHLVLTTLHTNDAA
GAIARLDEMGVEPFMISGALLGVLAQRLMRRVCSECKIAYVPTKEELARFGLSVSGEESVTFYKANTLSGEALQQAQANG
TVCQKCGGGGYKGRVGVYEFMVMSEELQKLINRGATTDMIKEKAVEEGMVTILAYSLNLVRQGHTTLEEVERVTFTDSGL
EAELKAKRKSSLTCSGCGAELQQEWLDCPYCMTPRFSE

Nucleotide


Download         Length: 2037 bp        

>NTDB_id=916400 VKI22_RS00985 WP_410765596.1 225938..227974(+) (pilB/pilB1) [Cyanobacterium aponinum UTEX 3221]
ATGACTCAAACTCCTATTTCTTCTGCTTCTAAAGAAAAACTAAAAACAGCCTTAGTCAGTCCAGATTATTTCAATCCCTT
TGGAAATAAACTAATTCAAGCAGGTTACATTGATAAGGAAGATTTAAAAAGGGCGTTGGTACAAGTGAGAAATACTAAAC
AGCCTTTAGTAAAAATCTTAGAAAATATCACCCACAAGGCTTTGTCTCCTGAGTTAGTGCGACAGTACAAACAACATCAA
CTTTTTGAATTAAAAATTCTTTATGGGGTTGATTGTGTTGACCCAGAGTCAGAAGAAATAGCAGCGGATCAAATGACCGA
TTTAATCGAGTCTATTATGGGAGTTCAGGTTTGTCGTCGTCATAAAGTTTTACCCTTGCGCCGCATTGAGGGTGAACCGC
CTGTAATTGTTGTTGCTATGGTTAATCCTGATGATTTACAGGCGTTGGATGATGTCAAAAAAATATTACGAGCCAAAGGC
CTTGACTTACAAAGAATTGTTATTACTGAAGAAGATTTTGATAAGTTAATTAATAGGTTTTATGAAGAAGATAAGCAAAG
ACAAAAAAGAGAAGAAGAGTTAAAAGAAAAGCAAAAATTAGATCAGTTAGGGGATGTTAGTGATATTGTTGATGATTTGG
ATCAGAATTTAGATGAAGCTGATGAAGAAAATCCAGATGGTGATTTGACTTCTAATGATGCTAATCAAGCCCCTATTATC
AAGTTAGTTAATAAAGTTCTCATCAAGGCTTTACAAGAAGGGGCATCAGACATTCATGTTGAACCCCAAGAAACTAGAAT
GAGGGTGCGTTTACGTAAAGATGGGGTTTTAAGGGAATATTTTACTCTACCTAAACATATTGCGACTGCGGTGGTTGCTC
GTTTCAAAATCATGGCTGAGTTGGATATTGCAGAACGCCGTTTACCTCAAGATGGAAAAATTCGTCGGGTGTTTCAGGGA
AGAAATGTGGATTTTCGAGTTAACTGTTTGCCTAGTCGTTACGGTGAAAAGGTCTGTTTAAGGATATTAGATAATTCGGC
TACTCAATTAGGTTTAGACTTTTTAATCTCGGACGAGGAAACATTGGCAAAAGTTAGAGATATTGCCAGTCGTCCTTTTG
GTTTAATTTTGGTTACAGGTCCTACAGGTTCGGGTAAGTCCACAACTCTCTATTCTGTCCTAGCGGAGAGAAATGATCCG
GGAGTGAACATAAGTACGGCGGAAGACCCTATTGAGTATGCTTTAGAAGGAATCACTCAGGTGCAAGTAATCCGAGAAAA
AGGTATGGATTTCGCTTCAATTCTTCGGGCTTTTCTCAGACAAGACCCTGATATTATTCTGGTGGGGGAAACACGGGACG
CTGAAACCGCAAAAACTGCGATCGAAGCCGCTTTGACTGGACACTTGGTTTTAACCACTCTACATACTAATGATGCGGCA
GGTGCGATCGCACGTTTGGACGAAATGGGGGTTGAACCCTTTATGATTTCTGGTGCATTGTTAGGGGTTTTGGCACAAAG
ATTAATGCGAAGAGTTTGTAGTGAGTGTAAAATTGCTTATGTTCCTACTAAAGAAGAATTAGCCCGTTTTGGTTTATCCG
TATCTGGAGAAGAATCTGTTACCTTTTATAAAGCGAATACTTTGTCAGGAGAAGCCCTACAACAAGCCCAAGCGAATGGC
ACGGTTTGCCAAAAATGTGGTGGCGGTGGTTATAAAGGGCGTGTGGGTGTTTATGAGTTTATGGTTATGAGTGAAGAGTT
GCAAAAACTCATTAACCGAGGAGCAACCACTGACATGATTAAAGAAAAGGCTGTTGAAGAAGGAATGGTAACGATTTTAG
CCTATAGTTTAAATCTAGTTCGTCAAGGTCATACAACCTTAGAAGAAGTTGAAAGGGTAACATTTACTGATTCTGGTTTG
GAAGCAGAATTGAAGGCTAAACGAAAATCATCTTTAACCTGTAGTGGCTGTGGAGCAGAATTACAACAGGAATGGTTAGA
TTGTCCTTATTGTATGACTCCTCGTTTCAGTGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(245-511)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(31-185)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

68.95

96.903

0.668
  pilF Thermus thermophilus HB27

41.374

92.33

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.968

92.625

0.37