Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   OCU36_RS12550 Genome accession   NZ_AP025458
Coordinates   2759007..2759606 (-) Length   199 a.a.
NCBI ID   WP_261838262.1    Uniprot ID   -
Organism   Vibrio artabrorum strain CECT 7226     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2754007..2764606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU36_RS12525 aroB 2754346..2755434 (-) 1089 WP_261838259.1 3-dehydroquinate synthase -
  OCU36_RS12530 aroK 2755460..2755978 (-) 519 WP_017061125.1 shikimate kinase AroK -
  OCU36_RS12535 pilQ 2756155..2757837 (-) 1683 WP_261838260.1 type IV pilus secretin PilQ Machinery gene
  OCU36_RS12540 pilP 2757898..2758416 (-) 519 WP_261839738.1 pilus assembly protein PilP Machinery gene
  OCU36_RS12545 pilO 2758403..2759014 (-) 612 WP_261838261.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCU36_RS12550 pilN 2759007..2759606 (-) 600 WP_261838262.1 PilN domain-containing protein Machinery gene
  OCU36_RS12555 pilM 2759590..2760615 (-) 1026 WP_261838263.1 type IV pilus assembly protein PilM Machinery gene
  OCU36_RS12560 - 2760767..2763328 (+) 2562 WP_261838264.1 penicillin-binding protein 1A -
  OCU36_RS12565 oxyR 2763466..2764359 (-) 894 WP_261838265.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 23175.68 Da        Isoelectric Point: 9.3674

>NTDB_id=91619 OCU36_RS12550 WP_261838262.1 2759007..2759606(-) (pilN) [Vibrio artabrorum strain CECT 7226]
MLHQINLLPWRDEIRVQHKRRFIHLLILGAIIAFVGQWAVGRYFHHQQVQQQHRLSYLSQYIADLERQIQLLKVAEQEHK
TILTRLDVVESLQRGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMAGISDSTARLATMLDNLERSDSLKGVEMHSIV
HNRKRFNKEFQTFKVSFVFSPMSLENTAANLEEKGANHG

Nucleotide


Download         Length: 600 bp        

>NTDB_id=91619 OCU36_RS12550 WP_261838262.1 2759007..2759606(-) (pilN) [Vibrio artabrorum strain CECT 7226]
ATGTTGCATCAAATTAACCTATTACCTTGGCGCGATGAGATTCGTGTTCAGCATAAAAGACGTTTTATTCATTTGCTGAT
TCTTGGGGCGATCATCGCTTTTGTTGGGCAATGGGCGGTGGGACGCTATTTTCATCACCAGCAAGTACAGCAGCAACATC
GCCTTAGTTATCTCAGTCAATACATTGCGGATCTCGAACGACAAATTCAGTTGTTAAAAGTCGCGGAGCAAGAACATAAA
ACCATTCTGACTCGACTCGATGTGGTGGAATCTTTACAGCGCGGCCGTAATAAAACCACCGATTTTATGAACTTGATGCC
AGAGCTGATCCCTGAAGGCGTTTATGTCGACAAGATCAAAATGAATGGTCAGGAGATTGAGATGGCGGGGATCAGTGACA
GTACCGCTCGTCTTGCCACTATGTTAGATAACTTAGAGCGTTCCGATTCCCTGAAAGGGGTTGAGATGCATTCTATTGTT
CACAATCGTAAACGTTTCAACAAGGAGTTCCAAACCTTCAAGGTCTCTTTTGTATTTAGCCCCATGTCTTTAGAAAATAC
CGCCGCAAACCTAGAAGAGAAGGGGGCTAATCATGGTTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

60.5

100

0.608
  pilN Vibrio campbellii strain DS40M4

63.441

93.467

0.593

Multiple sequence alignment