Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   U3P88_RS14925 Genome accession   NZ_CP140297
Coordinates   3094035..3094472 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain UMAF6639     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3089035..3099472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U3P88_RS14900 (U3P88_14900) - 3090056..3091192 (+) 1137 WP_061861140.1 zinc-dependent alcohol dehydrogenase -
  U3P88_RS14905 (U3P88_14905) - 3091207..3091641 (+) 435 WP_007409358.1 RDD family protein -
  U3P88_RS14910 (U3P88_14910) - 3091713..3092036 (+) 324 WP_060675310.1 YckD family protein -
  U3P88_RS14915 (U3P88_14915) - 3092140..3093575 (+) 1436 Protein_2899 family 1 glycosylhydrolase -
  U3P88_RS14920 (U3P88_14920) nin/comJ 3093616..3094014 (-) 399 WP_039252825.1 competence protein ComJ Regulator
  U3P88_RS14925 (U3P88_14925) nucA/comI 3094035..3094472 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  U3P88_RS14930 (U3P88_14930) hxlB 3094830..3095387 (-) 558 WP_039252823.1 6-phospho-3-hexuloisomerase -
  U3P88_RS14935 (U3P88_14935) hxlA 3095384..3096019 (-) 636 WP_031378686.1 3-hexulose-6-phosphate synthase -
  U3P88_RS14940 (U3P88_14940) - 3096251..3096613 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=913561 U3P88_RS14925 WP_003156588.1 3094035..3094472(-) (nucA/comI) [Bacillus velezensis strain UMAF6639]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=913561 U3P88_RS14925 WP_003156588.1 3094035..3094472(-) (nucA/comI) [Bacillus velezensis strain UMAF6639]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACATCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641