Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   U0N78_RS00780 Genome accession   NZ_CP140109
Coordinates   128805..129239 (+) Length   144 a.a.
NCBI ID   WP_043026797.1    Uniprot ID   A0A0Z8IB52
Organism   Streptococcus suis strain 2022WUSS148     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 123805..134239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0N78_RS00745 (U0N78_00745) - 124444..124803 (+) 360 WP_043026811.1 DUF1033 family protein -
  U0N78_RS00750 (U0N78_00750) - 124948..125898 (-) 951 WP_043026809.1 S66 family peptidase -
  U0N78_RS00755 (U0N78_00755) comYA 125984..126934 (+) 951 WP_043026808.1 competence type IV pilus ATPase ComGA Machinery gene
  U0N78_RS00760 (U0N78_00760) comYB 126846..127883 (+) 1038 WP_043026806.1 competence type IV pilus assembly protein ComGB Machinery gene
  U0N78_RS00765 (U0N78_00765) comYC 127885..128166 (+) 282 WP_043026803.1 competence type IV pilus major pilin ComGC Machinery gene
  U0N78_RS00770 (U0N78_00770) comGD 128147..128554 (+) 408 WP_043026801.1 competence type IV pilus minor pilin ComGD -
  U0N78_RS00775 (U0N78_00775) comGE 128598..128735 (+) 138 Protein_118 competence system putative prepilin ComGE -
  U0N78_RS00780 (U0N78_00780) comGF/cglF 128805..129239 (+) 435 WP_043026797.1 competence type IV pilus minor pilin ComGF Machinery gene
  U0N78_RS00785 (U0N78_00785) comGG 129217..129744 (+) 528 WP_043026795.1 competence type IV pilus minor pilin ComGG -
  U0N78_RS00790 (U0N78_00790) comYH 129801..130754 (+) 954 WP_043026794.1 class I SAM-dependent methyltransferase Machinery gene
  U0N78_RS00795 (U0N78_00795) - 130805..131992 (+) 1188 WP_043026792.1 acetate kinase -
  U0N78_RS00800 (U0N78_00800) - 132307..132858 (+) 552 WP_043026790.1 folate family ECF transporter S component -
  U0N78_RS00805 (U0N78_00805) - 132914..134170 (+) 1257 WP_256769122.1 bifunctional folylpolyglutamate synthase/dihydrofolate synthase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16462.85 Da        Isoelectric Point: 8.8126

>NTDB_id=912563 U0N78_RS00780 WP_043026797.1 128805..129239(+) (comGF/cglF) [Streptococcus suis strain 2022WUSS148]
MLKSKLPAFTLLECLVALIILSGSLLVFESLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGE
QALAFGKSRSDDFRKTNDRGQGYQPMIYQVDSAAISQKNQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=912563 U0N78_RS00780 WP_043026797.1 128805..129239(+) (comGF/cglF) [Streptococcus suis strain 2022WUSS148]
TTGTTAAAAAGTAAACTTCCTGCTTTTACTCTCTTGGAATGCTTGGTAGCTTTAATCATCCTATCAGGAAGTCTTCTGGT
CTTTGAAAGCTTGTCAAAACTGCTGGTCCAAGAAGCGCATTACCAAGGTCAGACTGTTCAAAAGGAATGGTTGGTCTTCT
CTAGTCAATTGCGGTCGGAGTGGGACCAGGCTGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGGCGAA
CAGGCTTTGGCCTTTGGCAAGTCACGGTCGGATGATTTTCGGAAAACAAATGACAGGGGACAGGGCTATCAGCCCATGAT
TTATCAAGTAGACAGCGCAGCTATTTCCCAAAAAAACCAGCTGGTGCGCATCGACTTTAGCTTTAAAAACGGAGAGGAGC
GGACGTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(37-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Z8IB52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae D39

53.901

97.917

0.528
  comGF/cglF Streptococcus pneumoniae R6

53.901

97.917

0.528
  comGF/cglF Streptococcus pneumoniae TIGR4

53.901

97.917

0.528
  comGF/cglF Streptococcus pneumoniae Rx1

53.901

97.917

0.528
  comYF Streptococcus mutans UA140

49.306

100

0.493
  comGF/cglF Streptococcus mitis NCTC 12261

52.985

93.056

0.493
  comYF Streptococcus mutans UA159

48.611

100

0.486
  comGF/cglF Streptococcus mitis SK321

51.493

93.056

0.479
  comGF Lactococcus lactis subsp. cremoris KW2

43.972

97.917

0.431