Detailed information NTG0001075

Experimentally validated

Overview

• Name: xcpV
• Type: Machinery gene
• Locus tag: ABD1_RS08070
• Genome accession: NC_020547
• Coordinates: 1680858..1681238 (+)
• Length: 126 a.a.
• NCBI ID: WP_000836911.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii D1279779
• Function: pseudopilus assembly DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.

Genomic Context

Location: 1675858..1686238

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABD1_RS08040 (ABD1_15660)	kdsB	1676761..1677522 (+)	762	WP_000680700.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
ABD1_RS08045 (ABD1_15670)	-	1677534..1678514 (+)	981	WP_043960937.1	DNA polymerase III subunit delta'	-
ABD1_RS08050 (ABD1_15680)	-	1678526..1678867 (+)	342	WP_001183024.1	PilZ domain-containing protein	-
ABD1_RS08055 (ABD1_15690)	-	1678885..1679658 (+)	774	WP_000497298.1	TatD family hydrolase	-
ABD1_RS08060 (ABD1_15700)	-	1679696..1680304 (+)	609	WP_000375838.1	TetR/AcrR family transcriptional regulator	-
ABD1_RS08065 (ABD1_15710)	-	1680314..1680868 (+)	555	WP_004841361.1	type II secretion system protein	-
ABD1_RS08070 (ABD1_15720)	xcpV	1680858..1681238 (+)	381	WP_000836911.1	type II secretion system minor pseudopilin GspI	Machinery gene
ABD1_RS08075 (ABD1_15730)	xcpW	1681238..1681939 (+)	702	WP_000594585.1	type II secretion system minor pseudopilin GspJ	Machinery gene
ABD1_RS08080 (ABD1_15740)	gspK	1681939..1682898 (+)	960	WP_000301468.1	type II secretion system minor pseudopilin GspK	-
ABD1_RS08085 (ABD1_15750)	-	1683128..1683724 (+)	597	WP_000908453.1	6-pyruvoyl trahydropterin synthase family protein	-
ABD1_RS08090 (ABD1_15760)	ung	1683775..1684488 (+)	714	WP_001177528.1	uracil-DNA glycosylase	-
ABD1_RS08095 (ABD1_15770)	-	1684485..1685609 (+)	1125	WP_001983908.1	enoyl-CoA hydratase/isomerase family protein	-
ABD1_RS08100 (ABD1_15780)	tadA	1685616..1686119 (+)	504	WP_001997771.1	tRNA adenosine(34) deaminase TadA	-

Sequence

Protein

• Length: 126 a.a.
• Molecular weight: 13975.20 Da
• Isoelectric Point: 9.8004

>NTDB_id=1075 ABD1_RS08070 WP_000836911.1 1680858..1681238(+) (xcpV) [Acinetobacter baumannii D1279779]
MKSKGFTLLEVMVALAIFAVAAVALTKVAMQYTQSTSNAILRTKAQFVAMNEVAMMEINQEWLQGTQSKQVTSQGETWQI
DKSAQSTISPNVQKIDLQVSLYDPDKGKVQNGITHMVFFNYPVKAK

Nucleotide

• Length: 381 bp

>NTDB_id=1075 ABD1_RS08070 WP_000836911.1 1680858..1681238(+) (xcpV) [Acinetobacter baumannii D1279779]
ATGAAATCTAAAGGCTTTACCCTCTTAGAAGTTATGGTTGCTTTGGCAATCTTTGCAGTCGCAGCTGTCGCTTTAACTAA
AGTGGCAATGCAGTACACGCAGTCCACTTCAAATGCAATTTTGCGAACCAAAGCTCAGTTTGTGGCAATGAATGAAGTTG
CTATGATGGAGATTAATCAAGAATGGTTGCAAGGAACTCAAAGTAAGCAGGTAACTTCCCAAGGTGAAACTTGGCAAATT
GATAAGTCAGCTCAATCTACAATTAGCCCAAATGTTCAAAAAATTGATTTACAAGTGAGTTTGTATGATCCGGATAAGGG
AAAAGTACAAAATGGCATTACTCATATGGTTTTTTTTAATTATCCAGTGAAAGCAAAATAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein I (T2SSI) (42-101)

• Prokaryotic N-terminal methylation motif (N_methyl) (2-25)

References

• [1]: Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB₂. Frontiers in Microbiology 12:738034. [PMID: 35126321]