Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   L6411_RS02045 Genome accession   NZ_AP025333
Coordinates   402179..403471 (+) Length   430 a.a.
NCBI ID   WP_237374311.1    Uniprot ID   -
Organism   Streptococcus ruminantium strain GUT-189     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 397179..408471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6411_RS02010 (GUT189_03600) - 397303..397674 (+) 372 WP_024532771.1 S1 RNA-binding domain-containing protein -
  L6411_RS02015 (GUT189_03610) - 397867..399123 (-) 1257 WP_237374021.1 ISL3 family transposase -
  L6411_RS02020 (GUT189_03620) - 399259..399489 (+) 231 WP_237374308.1 transposase -
  L6411_RS02025 (GUT189_03630) - 399486..400136 (+) 651 WP_237374310.1 IS3 family transposase -
  L6411_RS02030 - 400149..400370 (+) 222 WP_237374489.1 IS3 family transposase -
  L6411_RS02035 (GUT189_03640) cysK 400471..401397 (-) 927 WP_156018190.1 cysteine synthase A -
  L6411_RS02040 (GUT189_03650) - 401490..402122 (-) 633 WP_156018189.1 YigZ family protein -
  L6411_RS02045 (GUT189_03660) comFA/cflA 402179..403471 (+) 1293 WP_237374311.1 DEAD/DEAH box helicase Machinery gene
  L6411_RS02050 (GUT189_03670) - 403464..404129 (+) 666 WP_155974060.1 ComF family protein -
  L6411_RS02055 (GUT189_03680) hpf 404206..404748 (+) 543 WP_024533091.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 49022.61 Da        Isoelectric Point: 9.5866

>NTDB_id=91231 L6411_RS02045 WP_237374311.1 402179..403471(+) (comFA/cflA) [Streptococcus ruminantium strain GUT-189]
MNELQDYYGRLFTRNQLTAVERERAQTIPSITEKKTCFRCGTPFEEEYQLPNGAFYCRACLLLGRVRSDEKLYYFPQRDF
PKNTCLRWKGRLTNWQQTISDALCANVEKRQATLVHAVTGAGKTEMIYHTVASVIDSGGAVCLASPRIDVCIELYKRLQQ
DFSVPISLLHGESEAYFRTPMVIATTHQLLKFYRAFDLLLIDEVDAFPYADNPVLYRAVDNAVKPDGVQVFLTATSTDQL
EKKIKAGKLERLSLPRRFHGNPLVVPQKIWFSQFEQYLKKGRLTPKLAKVIQHQRKTGYPLLIFVPEIGKGMEFTKVVKK
AFPNENIGFVSSQTENRLEIVEGFRNKNITILISTTILERGVTFPCVDVFVIQANHYLYSSSSLVQIAGRVGRSQERPTG
LLHFFHKGSTRAIEKSIAEIKQMNKEAGYV

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=91231 L6411_RS02045 WP_237374311.1 402179..403471(+) (comFA/cflA) [Streptococcus ruminantium strain GUT-189]
ATGAATGAATTACAAGATTACTATGGAAGACTATTTACCAGGAATCAACTAACAGCAGTTGAGAGGGAAAGAGCGCAGAC
AATTCCAAGTATTACAGAGAAAAAAACTTGTTTCAGATGCGGAACTCCCTTTGAAGAAGAATACCAGTTGCCAAATGGAG
CTTTTTATTGCCGAGCCTGTTTGCTTTTAGGAAGGGTACGCTCGGACGAAAAACTTTACTATTTTCCACAGAGAGATTTT
CCTAAAAATACCTGCTTACGGTGGAAAGGACGACTGACCAATTGGCAACAAACCATTTCTGATGCTCTTTGCGCCAATGT
TGAGAAAAGGCAAGCTACGCTGGTTCATGCAGTAACAGGAGCTGGAAAAACAGAGATGATATATCATACAGTAGCTTCTG
TTATTGATAGTGGTGGAGCAGTTTGTTTAGCTAGTCCACGAATTGATGTTTGTATCGAACTCTATAAACGCTTGCAGCAG
GATTTTTCAGTTCCAATTAGTCTCTTGCACGGAGAATCAGAGGCCTACTTTAGAACCCCCATGGTCATTGCTACAACTCA
TCAGCTCTTAAAATTTTATCGTGCTTTTGATTTGCTTTTGATTGATGAAGTAGATGCCTTTCCATACGCAGATAATCCTG
TTCTTTATCGGGCAGTAGACAATGCAGTAAAACCAGATGGTGTACAGGTATTTTTGACAGCTACTTCAACAGATCAACTT
GAGAAAAAGATAAAAGCAGGAAAACTAGAACGCCTTAGTTTGCCCAGACGATTTCATGGCAATCCACTAGTAGTACCACA
AAAAATTTGGTTCAGCCAATTTGAACAGTATCTAAAGAAAGGTAGATTGACCCCTAAGCTGGCAAAGGTTATCCAGCATC
AGCGAAAGACAGGCTATCCCTTGCTAATATTTGTTCCAGAAATCGGAAAAGGGATGGAATTTACAAAGGTAGTCAAAAAA
GCATTTCCTAATGAAAACATTGGTTTTGTTTCCAGTCAGACAGAAAATCGCCTGGAGATAGTAGAAGGATTTCGCAATAA
AAACATCACCATTTTAATCTCAACTACCATTCTTGAACGCGGTGTAACCTTCCCTTGTGTTGATGTCTTTGTTATTCAGG
CCAATCATTATCTCTATAGTTCATCTAGTTTAGTTCAAATAGCAGGTCGAGTTGGTCGCAGCCAAGAACGACCAACAGGG
CTTCTACATTTTTTTCACAAAGGAAGCACGCGGGCGATTGAAAAGTCCATCGCTGAAATCAAACAAATGAATAAGGAGGC
AGGTTATGTCTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(291-394)

Type III restriction enzyme, res subunit (ResIII)

(92-235)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

64.554

99.07

0.64
  comFA/cflA Streptococcus pneumoniae D39

64.554

99.07

0.64
  comFA/cflA Streptococcus pneumoniae R6

64.554

99.07

0.64
  comFA/cflA Streptococcus pneumoniae TIGR4

64.554

99.07

0.64
  comFA/cflA Streptococcus mitis NCTC 12261

63.85

99.07

0.633
  comFA/cflA Streptococcus mitis SK321

63.146

99.07

0.626
  comFA Lactococcus lactis subsp. cremoris KW2

53.904

92.326

0.498
  comFA Latilactobacillus sakei subsp. sakei 23K

38.462

99.767

0.384

Multiple sequence alignment