Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   L6419_RS02180 Genome accession   NZ_AP025332
Coordinates   428119..429411 (+) Length   430 a.a.
NCBI ID   WP_155964074.1    Uniprot ID   -
Organism   Streptococcus ruminantium strain GUT-184     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 423119..434411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6419_RS02145 (GUT184_03870) - 423273..424673 (+) 1401 WP_155964758.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  L6419_RS02150 (GUT184_03880) - 424675..425046 (+) 372 WP_024532771.1 S1 RNA-binding domain-containing protein -
  L6419_RS02155 (GUT184_03890) - 425121..425441 (+) 321 WP_120171198.1 transposase -
  L6419_RS02160 (GUT184_03900) - 425438..425989 (+) 552 WP_237375601.1 IS3 family transposase -
  L6419_RS02165 - 425973..426314 (+) 342 WP_237375602.1 IS3 family transposase -
  L6419_RS02170 (GUT184_03910) cysK 426411..427337 (-) 927 WP_024533095.1 cysteine synthase A -
  L6419_RS02175 (GUT184_03920) - 427430..428062 (-) 633 WP_156020852.1 YigZ family protein -
  L6419_RS02180 (GUT184_03930) comFA/cflA 428119..429411 (+) 1293 WP_155964074.1 DEAD/DEAH box helicase Machinery gene
  L6419_RS02185 (GUT184_03940) - 429404..430069 (+) 666 WP_156020851.1 ComF family protein -
  L6419_RS02190 (GUT184_03950) hpf 430146..430688 (+) 543 WP_024533091.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48995.50 Da        Isoelectric Point: 9.3065

>NTDB_id=91178 L6419_RS02180 WP_155964074.1 428119..429411(+) (comFA/cflA) [Streptococcus ruminantium strain GUT-184]
MNELQDYYGRLFTRNQLTAVERERAQTIPSITEKKTCFRCGTPFEEEYQLPNGAFYCRACLLLGRVRSDEKLYYFPQRDF
PKNTCLQWKGRLTNWQQTISDALCANVEKRQATLVHAVTGAGKTEMIYHTVASVIDSGGAVCLASPRIDVCIELYKRLQQ
DFSVPISLLHGESEAYFRTPMVIATTHQLLKFYRAFDLLLIDEVDAFPYADNPVLYRAVDNAVKPDGVQVFLTATSTDQL
EKKIKAGKLERLSLPRRFHGNPLVVPQKIWFSQFEQYLKKGRLTPKLAKVIQHQRKTGYPLLIFVPEIGKGMEFTKVVKK
AFPNENIGFVSSQTENRLEIVEGFRNKNITILISTTILERGVTFPCVDVFVIQANHYLYSSSSLVQIAGRVGRSQERPTG
LLHFFHEGSTRAIEKSIAEIKQMNKEAGYV

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=91178 L6419_RS02180 WP_155964074.1 428119..429411(+) (comFA/cflA) [Streptococcus ruminantium strain GUT-184]
ATGAATGAATTACAAGATTACTATGGAAGACTATTTACCAGGAATCAACTAACAGCAGTTGAGAGGGAAAGAGCGCAGAC
AATTCCAAGTATTACAGAGAAAAAAACTTGTTTCAGATGCGGAACTCCCTTTGAAGAAGAATACCAGTTGCCAAATGGAG
CTTTTTATTGTCGAGCCTGTTTGCTTTTAGGAAGGGTACGCTCGGACGAAAAACTTTACTATTTTCCACAGAGAGATTTT
CCTAAAAATACCTGCTTACAGTGGAAAGGACGACTGACCAATTGGCAACAAACCATTTCTGATGCTCTTTGCGCCAATGT
TGAGAAAAGGCAAGCTACGCTGGTTCATGCAGTAACAGGAGCTGGAAAAACAGAGATGATATATCATACAGTAGCTTCCG
TTATTGATAGTGGTGGAGCAGTTTGTTTAGCCAGTCCACGAATTGATGTTTGTATCGAACTCTATAAACGCTTGCAGCAG
GATTTTTCAGTTCCAATTAGTCTCTTGCACGGAGAATCAGAGGCCTACTTTAGAACCCCCATGGTCATTGCTACAACTCA
TCAGCTCTTAAAATTTTATCGTGCTTTTGATTTGCTTTTGATTGATGAAGTAGATGCCTTTCCATACGCAGATAATCCTG
TTCTTTATCGGGCAGTAGACAATGCAGTAAAACCAGATGGTGTACAGGTATTTTTGACAGCTACTTCAACAGATCAACTT
GAGAAAAAGATAAAAGCAGGAAAACTAGAACGCCTTAGTTTGCCCAGACGATTTCATGGTAATCCACTAGTAGTACCACA
AAAAATTTGGTTCAGCCAATTTGAACAGTATCTAAAGAAAGGTAGATTGACCCCTAAGCTGGCAAAGGTTATCCAGCATC
AGCGAAAGACAGGCTATCCCTTGCTAATATTTGTTCCAGAAATCGGAAAAGGGATGGAATTTACAAAGGTAGTCAAAAAA
GCATTTCCTAATGAAAACATTGGTTTTGTTTCCAGTCAGACAGAAAATCGCCTGGAGATAGTAGAAGGATTTCGCAATAA
AAACATCACCATTTTAATCTCAACCACCATTCTTGAACGCGGTGTAACCTTCCCTTGTGTTGATGTCTTTGTTATTCAGG
CCAATCATTATCTCTATAGTTCATCTAGTTTAGTTCAAATAGCAGGTCGAGTTGGTCGCAGCCAAGAACGACCAACAGGG
CTTCTACATTTTTTTCACGAAGGAAGCACGCGGGCGATTGAAAAGTCCATCGCTGAAATCAAACAAATGAATAAGGAGGC
AGGTTATGTCTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(92-235)

Helicase conserved C-terminal domain (Helicase_C)

(291-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

64.554

99.07

0.64
  comFA/cflA Streptococcus pneumoniae D39

64.554

99.07

0.64
  comFA/cflA Streptococcus pneumoniae R6

64.554

99.07

0.64
  comFA/cflA Streptococcus pneumoniae TIGR4

64.554

99.07

0.64
  comFA/cflA Streptococcus mitis NCTC 12261

63.85

99.07

0.633
  comFA/cflA Streptococcus mitis SK321

63.146

99.07

0.626
  comFA Lactococcus lactis subsp. cremoris KW2

53.652

92.326

0.495
  comFA Latilactobacillus sakei subsp. sakei 23K

38.462

99.767

0.384

Multiple sequence alignment