Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABWI18_RS10650 Genome accession   NZ_CP159455
Coordinates   2170996..2171436 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain S6aCHROMc     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2165996..2176436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABWI18_RS10630 (ABWI18_10645) aroP 2166994..2168367 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  ABWI18_RS10635 (ABWI18_10650) ampE 2168410..2169264 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  ABWI18_RS10640 (ABWI18_10655) ampD 2169261..2169812 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ABWI18_RS10645 (ABWI18_10660) nadC 2169900..2170793 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ABWI18_RS10650 (ABWI18_10665) pilA 2170996..2171436 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  ABWI18_RS10655 (ABWI18_10670) pilB 2171446..2172831 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  ABWI18_RS10660 (ABWI18_10675) hofC 2172821..2174023 (+) 1203 WP_000157236.1 protein transport protein HofC -
  ABWI18_RS10665 (ABWI18_10680) guaC 2174058..2175101 (-) 1044 WP_001217338.1 GMP reductase -
  ABWI18_RS10670 (ABWI18_10685) - 2175257..2175301 (-) 45 WP_120795372.1 protein YacM -
  ABWI18_RS10675 (ABWI18_10690) coaE 2175326..2175946 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=911462 ABWI18_RS10650 WP_000360895.1 2170996..2171436(+) (pilA) [Escherichia coli strain S6aCHROMc]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=911462 ABWI18_RS10650 WP_000360895.1 2170996..2171436(+) (pilA) [Escherichia coli strain S6aCHROMc]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363