Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   UM396_RS12350 Genome accession   NZ_CP139723
Coordinates   2411427..2412497 (-) Length   356 a.a.
NCBI ID   WP_063165283.1    Uniprot ID   -
Organism   Geobacillus subterraneus strain OXR-76     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2406427..2417497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UM396_RS12310 (UM396_12310) - 2407263..2408057 (+) 795 WP_322310279.1 YqhG family protein -
  UM396_RS12315 (UM396_12315) - 2408133..2408312 (-) 180 WP_081209484.1 YqzE family protein -
  UM396_RS12320 (UM396_12320) comGG 2408358..2408747 (-) 390 WP_168368781.1 competence type IV pilus minor pilin ComGG -
  UM396_RS12325 (UM396_12325) comGF 2408878..2409321 (-) 444 WP_168368782.1 competence type IV pilus minor pilin ComGF -
  UM396_RS12330 (UM396_12330) - 2409300..2409626 (-) 327 WP_063165285.1 hypothetical protein -
  UM396_RS12335 (UM396_12335) comGD 2409610..2410053 (-) 444 WP_225995614.1 competence type IV pilus minor pilin ComGD -
  UM396_RS12340 (UM396_12340) comGC 2410037..2410333 (-) 297 WP_082816525.1 competence type IV pilus major pilin ComGC Machinery gene
  UM396_RS12345 (UM396_12345) comGB 2410402..2411430 (-) 1029 WP_168368783.1 competence type IV pilus assembly protein ComGB -
  UM396_RS12350 (UM396_12350) comGA 2411427..2412497 (-) 1071 WP_063165283.1 competence type IV pilus ATPase ComGA Machinery gene
  UM396_RS12355 (UM396_12355) - 2412698..2413396 (+) 699 WP_168368784.1 metalloregulator ArsR/SmtB family transcription factor -
  UM396_RS12360 (UM396_12360) - 2413558..2413800 (+) 243 WP_008879854.1 DUF2626 family protein -
  UM396_RS12365 (UM396_12365) - 2413831..2414958 (-) 1128 WP_063165281.1 class I SAM-dependent methyltransferase -
  UM396_RS12370 (UM396_12370) - 2415187..2415819 (-) 633 WP_063165280.1 MBL fold metallo-hydrolase -
  UM396_RS12375 (UM396_12375) - 2415999..2416175 (+) 177 WP_081209504.1 DUF2759 domain-containing protein -
  UM396_RS12380 (UM396_12380) - 2416211..2417374 (-) 1164 WP_322310280.1 M14 family metallopeptidase -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39500.69 Da        Isoelectric Point: 8.9679

>NTDB_id=910726 UM396_RS12350 WP_063165283.1 2411427..2412497(-) (comGA) [Geobacillus subterraneus strain OXR-76]
MDDIEQVANRLLAEAVQRRASDLHLVPRRHDAAIRLRLDGTLTDVGALPKETAERVIVHFKFLAGMDIGERRRPQSGAME
VNESGETVYLRLSTLPTLYDESLVIRLLPQRFSLPLRELSLFPQSTARLFSFMQQPQGLVLLTGPTGSGKTTTLYTLLDV
CQAERQRNIITLEDPIEKRNDRFLQVQINEKAGITYAASLKAALRHDPDVLMVGEIRDHDTAAIAVRSALTGHLVVSTMH
AADAVGAVYRLHEFGVPPGDLAETLLAVSAQRLVELCCPLCGDDCHPACRQLRRRRRAAVHELLYGPALLDAIRSLSDGD
GRRLRRHMTLARLIRKGIALGYLPVRALRLVGGEGR

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=910726 UM396_RS12350 WP_063165283.1 2411427..2412497(-) (comGA) [Geobacillus subterraneus strain OXR-76]
CTGGATGATATCGAACAAGTAGCGAACCGTCTCCTCGCTGAAGCGGTGCAGCGCCGCGCCTCTGATCTTCACCTCGTCCC
GCGCCGCCATGATGCGGCCATTCGCCTCCGTCTTGACGGCACGCTCACCGATGTCGGCGCGCTCCCGAAAGAAACCGCTG
AACGCGTCATCGTCCATTTCAAGTTTCTAGCCGGCATGGATATCGGCGAACGCCGCCGACCGCAAAGCGGCGCGATGGAA
GTGAATGAGTCCGGGGAAACGGTGTATTTGCGCTTATCGACGCTGCCGACGCTTTACGATGAAAGCCTCGTCATCCGCCT
TCTGCCGCAGCGCTTTTCGCTGCCGCTTCGCGAACTATCCTTATTTCCTCAATCCACCGCACGGTTGTTTTCCTTTATGC
AGCAGCCGCAAGGGCTCGTACTGTTGACCGGACCGACCGGGTCGGGAAAGACGACGACATTGTATACGCTTCTTGACGTT
TGCCAAGCGGAAAGACAACGCAACATCATCACGCTGGAAGATCCGATCGAAAAACGGAATGACCGGTTTTTGCAAGTGCA
GATCAATGAAAAAGCGGGCATCACGTATGCGGCGAGCTTGAAAGCTGCTTTGCGCCATGACCCGGACGTGTTAATGGTCG
GCGAGATCCGCGACCATGATACGGCGGCGATCGCCGTGCGATCGGCGTTAACAGGACATTTAGTCGTGTCGACCATGCAT
GCTGCCGATGCGGTCGGCGCGGTGTACCGGCTGCATGAGTTCGGGGTGCCGCCCGGCGATTTAGCGGAGACGTTGCTCGC
CGTTTCGGCGCAGCGTCTCGTTGAACTGTGCTGTCCGCTATGCGGCGACGACTGCCATCCGGCGTGTCGCCAGCTTCGAC
GCAGACGGCGCGCTGCCGTCCATGAACTGCTTTACGGCCCGGCGCTTCTTGATGCCATCCGTTCACTGTCGGACGGAGAC
GGGCGACGGCTCCGCCGCCATATGACATTAGCCCGCCTCATCCGCAAAGGAATCGCGCTCGGTTATTTGCCGGTTCGCGC
GCTGAGACTTGTCGGAGGGGAAGGACGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

51.884

96.91

0.503