Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   SNE23_RS26050 Genome accession   NZ_CP139483
Coordinates   4977605..4978954 (-) Length   449 a.a.
NCBI ID   WP_128805256.1    Uniprot ID   -
Organism   Bacillus sp. RA(2023)     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4972605..4983954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SNE23_RS26015 (SNE23_26015) - 4972642..4973337 (-) 696 WP_048528145.1 hypothetical protein -
  SNE23_RS26020 (SNE23_26020) - 4973324..4974112 (-) 789 WP_048528144.1 hypothetical protein -
  SNE23_RS26025 (SNE23_26025) - 4974144..4974713 (-) 570 WP_048528143.1 hypothetical protein -
  SNE23_RS26030 (SNE23_26030) - 4974831..4975313 (-) 483 WP_088313534.1 hypothetical protein -
  SNE23_RS26035 (SNE23_26035) hpf 4975714..4976256 (-) 543 WP_048528141.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SNE23_RS26040 (SNE23_26040) cspC 4976577..4976774 (-) 198 WP_001990088.1 cold shock protein CspC -
  SNE23_RS26045 (SNE23_26045) - 4976901..4977605 (-) 705 WP_048528140.1 ComF family protein -
  SNE23_RS26050 (SNE23_26050) comFA 4977605..4978954 (-) 1350 WP_128805256.1 ATP-dependent helicase ComFA Machinery gene
  SNE23_RS26055 (SNE23_26055) - 4979080..4980520 (-) 1441 Protein_5065 NlpC/P60 family protein -
  SNE23_RS26060 (SNE23_26060) - 4980660..4980974 (-) 315 WP_000400857.1 winged helix-turn-helix transcriptional regulator -
  SNE23_RS26065 (SNE23_26065) - 4981147..4981989 (-) 843 WP_048528137.1 DegV family protein -
  SNE23_RS26070 (SNE23_26070) - 4982230..4982865 (+) 636 WP_048528136.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51266.82 Da        Isoelectric Point: 9.6635

>NTDB_id=910016 SNE23_RS26050 WP_128805256.1 4977605..4978954(-) (comFA) [Bacillus sp. RA(2023)]
MLAGKQLLLEELSSDLRKELSDLKKKGEVACVQGITKNASKYICQRCGNIEQRLFASFLCKRCSKRCTYCRKCITMGRVS
ECTVLVRGIQERKEERELNQLQWKGVLSTGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIAEALQKGERVCIAT
PRTDVVLELAPRLQEVFPNINIAALYGGSTDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKRNFRKGNQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLQHKKIPRVLLQWLKIYVNKKHPIFL
FVPHVRYIEEISLLLKELDNRIDGVHAEDSMRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVMYFHYGKTESMVRAKKHILSMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=910016 SNE23_RS26050 WP_128805256.1 4977605..4978954(-) (comFA) [Bacillus sp. RA(2023)]
ATGCTTGCTGGAAAACAGTTGCTATTAGAAGAACTCTCTTCAGATTTACGGAAAGAATTAAGTGACTTGAAAAAGAAGGG
AGAAGTCGCATGTGTACAAGGCATAACAAAGAATGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGAT
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAAGATGCACGTATTGCCGGAAGTGCATCACGATGGGGAGAGTTAGT
GAATGTACTGTACTCGTTCGTGGGATTCAGGAAAGAAAGGAAGAAAGAGAGTTAAATCAGTTGCAGTGGAAAGGGGTTTT
GTCTACCGGTCAGGAGTTGGCGGCGCAAGGTGTTATAGAAGCTATTAAGCAGAAAGAATCCTTCTTTATTTGGGCTGTAT
GCGGTGCTGGAAAAACAGAAATGTTATTTTACGGTATTGCAGAGGCACTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCAAGAACAGACGTTGTACTGGAACTAGCACCGAGATTACAAGAAGTGTTTCCTAATATAAATATAGCTGCTTTATACGG
AGGGAGTACAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACGCATCAATTGTTACGTTATTATAGAGCGTTTC
ATGTCATGATTGTAGATGAAATTGATGCTTTCCCGTATCACGCAGATCAAATGTTACAGTATGCGGTGCAACAAGCGATG
AAAGAGAAAGCGGCGCGTATTTATTTAACAGCAACTCCTGATGAAAAGTGGAAGCGTAATTTCAGAAAGGGGAATCAAAA
AGGTATCATTGTCTCAGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGCTGGTGCGGAAATTGGAAGAAAA
GCCTTCAGCATAAAAAAATTCCTCGCGTGTTACTACAATGGTTAAAAATATACGTAAACAAAAAACATCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATAAGTCTGTTATTGAAAGAATTGGATAATAGAATCGATGGTGTACATGC
AGAAGATTCGATGAGAAAAGAAAAAGTAGCAGCGTTCAGAAAGGGAGAAATTCCGTTATTAGTTACGACAACAATTTTAG
AAAGGGGAGTAACTGTGAAGAATTTACAAGTGGCGGTGCTAGGGGCAGAAGAAGAAATTTTTTCAGAGAGTGCGCTCGTA
CAAATTGCAGGCCGGGCGGGTCGTAGTTTTGAAGAGCCATATGGCGAGGTTATGTATTTTCATTACGGTAAGACAGAGTC
GATGGTACGCGCGAAAAAACACATTTTAAGTATGAACAAAAATGCGAAGGAACAAGGGTTAATCGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(308-408)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.357

98.441

0.506
  comFA/cflA Streptococcus mitis SK321

37.844

97.105

0.367
  comFA Latilactobacillus sakei subsp. sakei 23K

40.494

90.2

0.365