Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   SNE23_RS05665 Genome accession   NZ_CP139483
Coordinates   1092862..1096587 (+) Length   1241 a.a.
NCBI ID   WP_157409460.1    Uniprot ID   -
Organism   Bacillus sp. RA(2023)     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1087862..1101587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SNE23_RS05650 (SNE23_05650) - 1088020..1088613 (+) 594 WP_048526648.1 TVP38/TMEM64 family protein -
  SNE23_RS05655 (SNE23_05655) lepB 1088670..1089233 (+) 564 WP_048526650.1 signal peptidase I -
  SNE23_RS05660 (SNE23_05660) addB 1089350..1092865 (+) 3516 WP_157409459.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  SNE23_RS05665 (SNE23_05665) addA 1092862..1096587 (+) 3726 WP_157409460.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  SNE23_RS05670 (SNE23_05670) - 1096600..1096887 (+) 288 WP_000255723.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  SNE23_RS05675 (SNE23_05675) - 1096981..1097358 (-) 378 WP_048526657.1 DUF1433 domain-containing protein -
  SNE23_RS05680 (SNE23_05680) - 1097417..1098586 (+) 1170 WP_071758310.1 hypothetical protein -
  SNE23_RS05685 (SNE23_05685) - 1098597..1098977 (+) 381 WP_098344763.1 hypothetical protein -
  SNE23_RS05690 (SNE23_05690) gerPF 1099256..1099471 (-) 216 WP_001141566.1 spore germination protein GerPF -
  SNE23_RS05695 (SNE23_05695) - 1099514..1099900 (-) 387 WP_157409462.1 spore germination protein GerPE -
  SNE23_RS05700 (SNE23_05700) gerPD 1099916..1100110 (-) 195 WP_001102342.1 spore germination protein GerPD -
  SNE23_RS05705 (SNE23_05705) gerPC 1100117..1100731 (-) 615 WP_048526666.1 spore germination protein GerPC -
  SNE23_RS05710 (SNE23_05710) gerPB 1100800..1101006 (-) 207 WP_001012508.1 spore germination protein GerPB -
  SNE23_RS05715 (SNE23_05715) gerPA 1101021..1101242 (-) 222 WP_001111188.1 spore germination protein GerPA -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142677.78 Da        Isoelectric Point: 4.8477

>NTDB_id=909983 SNE23_RS05665 WP_157409460.1 1092862..1096587(+) (addA) [Bacillus sp. RA(2023)]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVRFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEHSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVPDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKHFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPAEIYGYDTSWK
VEVVDGSTLLAPEPAQEEKQELLEALREKKAVPLQSERKDEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIQTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQINGMVNKELLTFEQAEEIAIEKIISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=909983 SNE23_RS05665 WP_157409460.1 1092862..1096587(+) (addA) [Bacillus sp. RA(2023)]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCTGGTTCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAACCAGGCTCTCAGCACATTAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCTATTTCGACGATCCATTCCTTTTGTTTACAAGTAATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACGGAAAATGAATTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGGATTGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGATCGTAGCGATGATGACTTACAGCGTATGATTTTAGC
ACTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAGTGGCTCGATAAATTAGTAGAAGCATATGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTATTTATTAGAAGATGTGAGATTCCAGCTTGAAACAGCGGAACAGCATATT
CGGAAAGCGACGGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACACTGCAAGCAGATTTAGCTTTACT
TGGAACGTTATCATCAGCCGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATATTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGTCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACATAGTGAAAACGGTGAAATGAATCCGTCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCTATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTAGGTGACGTAAAACAGTCAATTTATCGTTTCCGACTAGCAG
AACCAGGTTTATTCCTAGGAAAGTATAAACGCTTTACACAAGAAGGATTGGGCGGCGGAATGAAGATAGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGCTAGCAGGTACGAACTTTATCTTCAAACAAATTATGGGTGAAGAAGTCGGAGAAAT
CGACTACGATGCAGACGCTGAATTAAAGTTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGTA
TTCAGCAAACGGAAGAAGAAGTGCCAGACGGTGAAGAAGGTGCCGAAGTAGAAAAAGCACAGCTTGAAGCTCGACTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGACCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGACTTCGTTATTTTGCTTCGCTCTATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAGTTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATAATGATGAACGTATTCCGCGTGATCGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTGCTTCGTTCACCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCCCACGGAAAGAAAGGTTCGTTTTATGAAGTGATGAGCTCATTCTTAAAAGGAGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTACTACAAGGATGGCGTGAATTCGCACGTCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTTGTCGGTGGTTTACCAGCCGGAAAGCAAAGACAGGCAAACTT
GCGCGTATTATATGACCGCGCAAGACAATACGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGACGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGACGTCGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTTGAGTTCCCAGTCGTATTCGTAGCGGGGCTTGGTCGCCGTTTTAACACACAAGATTTAATGAA
ACATTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGTATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATACGTAGCGTTAACGCGT
GCAAAAGAAAAGTTAATTTTAATTGGAACAGTGAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGAGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGTAGTATTCCGGCTGAAATTTATGGATACGACACTAGCTGGAAA
GTAGAAGTTGTTGACGGTAGCACGTTACTTGCGCCAGAACCAGCTCAAGAAGAGAAACAAGAATTACTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCCCTGCAAAGTGAACGAAAAGATGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCATCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTGCGTGCGCCAATTCAAACACGCCCTCGTTTTATGGAGAAAAAAGGATTAACGTACGCTGAAAGAGG
TACGGCAGTTCACGCTGTTATGCAGCATGTTGATTTGAAGAAGCCAATTACAGAAGAAGTGATTCGGGAACAAATTAATG
GCATGGTCAATAAAGAGCTATTAACATTCGAACAAGCAGAAGAAATAGCAATCGAAAAAATCATTTCATTCTTTGACAGT
GATCTAGGTAAAAGAGTATTAGCGGCGAAAAGCGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGAAAGAGCGGGGAATCAATTCTTGTTCAAGGGGTTATTGACTGTATGATTGAAGAAGAAGACG
GTATTACGTTAATCGATTTTAAAACGGATACGATTGAAGGGAAGTTCCCAGGCGGATTTGATCAAGCGAAACCAATTTTA
GAAGATCGATATAAAGTGCAACTTTCTTTATATGCAAAGGCACTTGAAAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATATTTCTTTGATGGGAATCACGTTGTAAATATTGAAGAATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.828

100

0.538