Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   SNE23_RS05660 Genome accession   NZ_CP139483
Coordinates   1089350..1092865 (+) Length   1171 a.a.
NCBI ID   WP_157409459.1    Uniprot ID   A0AAU0QXX8
Organism   Bacillus sp. RA(2023)     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1084350..1097865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SNE23_RS05625 (SNE23_05625) cspA 1085202..1085405 (+) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  SNE23_RS05630 (SNE23_05630) - 1085508..1085783 (+) 276 WP_071758315.1 hypothetical protein -
  SNE23_RS05635 (SNE23_05635) - 1085809..1085994 (-) 186 WP_157409458.1 hypothetical protein -
  SNE23_RS05640 (SNE23_05640) - 1086279..1086860 (+) 582 WP_048526644.1 competence protein ComK -
  SNE23_RS05645 (SNE23_05645) - 1087102..1087698 (+) 597 WP_071758313.1 DUF4306 domain-containing protein -
  SNE23_RS05650 (SNE23_05650) - 1088020..1088613 (+) 594 WP_048526648.1 TVP38/TMEM64 family protein -
  SNE23_RS05655 (SNE23_05655) lepB 1088670..1089233 (+) 564 WP_048526650.1 signal peptidase I -
  SNE23_RS05660 (SNE23_05660) addB 1089350..1092865 (+) 3516 WP_157409459.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  SNE23_RS05665 (SNE23_05665) addA 1092862..1096587 (+) 3726 WP_157409460.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  SNE23_RS05670 (SNE23_05670) - 1096600..1096887 (+) 288 WP_000255723.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  SNE23_RS05675 (SNE23_05675) - 1096981..1097358 (-) 378 WP_048526657.1 DUF1433 domain-containing protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134383.50 Da        Isoelectric Point: 5.1777

>NTDB_id=909982 SNE23_RS05660 WP_157409459.1 1089350..1092865(+) (addB) [Bacillus sp. RA(2023)]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGEIILYLVPEQMTFQTQQALISSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTLDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSQSLAHLETHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTDGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TYLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHKFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=909982 SNE23_RS05660 WP_157409459.1 1089350..1092865(+) (addB) [Bacillus sp. RA(2023)]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGTCCAAGAGGGGAAATAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCATTAATTAGTA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCAGGCGTACATATGTTACTTCGTAAAATTGTAGAATCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGTAGTATGATTGCGGAGTTTAAACGTTACA
ACGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAGCAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTACTATATGATGATTTTGAGCGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
CTTGCAATTGCTAGTCGAAAAGCTTCCGCAGTCTGAATATGTAAATGGGGCTGAAATTTATATAGATGGATTTCATTCAT
TCTCACCGCAAGAGCTAGAAATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTGACAATCACGTTAACACTAGATGAA
AAAACGTTAGCACAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
ACGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCGCTTATGGAGCAACCGCGTTTTCATTCGCAATCATTAGCGCATT
TAGAAACGCATTACGAAGCTCGTCCAAATGAAAAGTTTCACGGAGAAGCGAGTGTAACGATTAGTACAGCAGCGAATTTA
CGAGCTGAAGTAGAAGGCGTTGCGCGTGAAATTCGTAGACTTGTGGCAGATGAAGACTATCGTTACCGAGATATCGCGGT
ACTTCTTCGTAACGGGGAAAGTTATTATGATGTAATGCGAACGCTATTTACAGATTATAATATCCCGCACTTCATTGATG
AAAAGCGCCCGATGTCACATCATCCGCTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTGTTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCTTATGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACGGACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACGCCTGTTATTCGTATGCAAAAAAGACTGAAGCGTGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTTGACGTTCCGAAAAAGCTAGAAGCATTACGTATTCGTGCAGAAGAGAGCGGAGATTTCTTAT
TCGCGACAGATCACGAGCAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACGTTCGTAGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCCAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCGAATATTGATCGTTCAAGATTATCAAATGTGAAAGCAACATTTGTCATTGGCGTAAATGAAGGAG
TTATTCCAGCAGCACCGATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGACGTTCTTAGTGCTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
CATTTCTTGTCCGCTTGCAGATGAGGAAGGAAAGACGTTACTTGCTTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAATGACGTAAATGATTTATCCCGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTATCTTACGTTATGCAGCAACTTCAAACGTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAACTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGCTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGTTGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAACCGTTGCGCATATGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTCAAACTGGATGC
GCCAGATATCGGCGAATTATTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAATCGTACTTGGGCAG
ACTTATCAATAAAAGAATGCGAGCATCTTTCTGTTTTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGCCATTTCTATTTAAAACAAAAATTGCAGCAAATCATTTTCCGTACGTCAATCATTCTACGTGAACA
TGCGAAATCAAGTGGTTTCGTACCAGTAGATTTAGAAGTACCGTTCGGTATGGGCGGTACAGGATCGCTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGG
ACATACCTCCGTATTATTGATTATAAATCGAGCTCAAAAGCGTTAGATTTAACGGAAGTGTACTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAAGGCGGCACGGCTTCACCGGCTGGTG
TACTGTACTTCCATATTCATAACCCAATCGTTGAGATGAAAGGTGACGCATCTGAAGCGGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTCGTGCTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTGCAAAAATATGTACACCATAAGTTTGAAAATATCGGAAAAGATATTACAGAAGGCGTGATCGATATTGCCCCT
TATAAAAAAGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCTGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAATTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(302-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.063

100

0.492